Detailed Information on Publication Record

PAZOUREK, Jiří, Lucie NYTROVÁ and Klára ODEHNALOVÁ. Extension of the Internal Standard Method for Determination of Thermodynamic Acidity Constants of Compounds Sparingly Soluble in Water by Capillary Zone Electrophoresis. ACS Omega. WASHINGTON: AMER CHEMICAL SOC, 2021, vol. 7, No 1, p. 1477-1482. ISSN 2470-1343. Available from: https://dx.doi.org/10.1021/acsomega.1c06224.

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Basic information
Original name	Extension of the Internal Standard Method for Determination of Thermodynamic Acidity Constants of Compounds Sparingly Soluble in Water by Capillary Zone Electrophoresis
Authors	PAZOUREK, Jiří (203 Czech Republic, guarantor, belonging to the institution), Lucie NYTROVÁ (203 Czech Republic, belonging to the institution) and Klára ODEHNALOVÁ (203 Czech Republic, belonging to the institution).
Edition	ACS Omega, WASHINGTON, AMER CHEMICAL SOC, 2021, 2470-1343.

Other information
Original language	English
Type of outcome	Article in a journal
Field of Study	10406 Analytical chemistry
Country of publisher	United States of America
Confidentiality degree	is not subject to a state or trade secret
WWW	URL
Impact factor	Impact factor: 4.132
RIV identification code	RIV/00216224:14160/21:00124011
Organization unit	Faculty of Pharmacy
Doi	http://dx.doi.org/10.1021/acsomega.1c06224
UT WoS	000739325200001
Keywords in English	HIGH-THROUGHPUT METHOD;DISSOCIATION-CONSTANTS;PK(A) DETERMINATION;MOBILITIESVALUES;DRUGS
Tags	rivok, ÚChL
Changed by	Changed by: JUDr. Sabina Krejčiříková, učo 383857. Changed: 14/4/2022 12:53.

Abstract
The paper extends applicability of the internal standard method published in 2009 (Fuguet E. et al., J. Chromatogr. A 2009, 1216(17), 3646). Although the original capillary zone electrophoresis method was suggested to determine thermodynamic acidity constants of compounds sparingly soluble in aqueous solutions by carrying out only runs at two different pH values (i.e., without the need to perform many experiments over the appropriate pH range including the form of a low-ionized analyte), we proved that the approach also virtually overcomes any interactions of the analyte in mixed solvents, so that the experiments can be carried out in a methanol-water buffer where the solubility is much better. Applicability of the extended method is illustrated on six selected beta-blockers.

Abstract

The paper extends applicability of the internal standard method published in 2009 (Fuguet E. et al., J. Chromatogr. A 2009, 1216(17), 3646). Although the original capillary zone electrophoresis method was suggested to determine thermodynamic acidity constants of compounds sparingly soluble in aqueous solutions by carrying out only runs at two different pH values (i.e., without the need to perform many experiments over the appropriate pH range including the form of a low-ionized analyte), we proved that the approach also virtually overcomes any interactions of the analyte in mixed solvents, so that the experiments can be carried out in a methanol-water buffer where the solubility is much better. Applicability of the extended method is illustrated on six selected beta-blockers.