Visualization of hydrogen atoms in a perdeuterated
lectin-fucose complex reveals key details of
protein-carbohydrate interactions

J 2021

Visualization of hydrogen atoms in a perdeuterated lectin-fucose complex reveals key details of protein-carbohydrate interactions

GAJDOŠ, Lukáš, Matthew P. BLAKELEY, Atul KUMAR, Michaela WIMMEROVÁ, Michaela HAERTLEIN et. al.

Basic information

Original name

Visualization of hydrogen atoms in a perdeuterated lectin-fucose complex reveals key details of protein-carbohydrate interactions

Authors

GAJDOŠ, Lukáš (703 Slovakia), Matthew P. BLAKELEY (250 France), Atul KUMAR (356 India, belonging to the institution), Michaela WIMMEROVÁ (203 Czech Republic, guarantor, belonging to the institution), Michaela HAERTLEIN (250 France), Trevor V. FORSYTH (250 France), Anne IMBERTY (250 France) and Juliette M. DEVOS (250 France)

Edition

Structure, CAMBRIDGE, CELL PRESS, 2021, 0969-2126

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 5.871

RIV identification code

RIV/00216224:14310/21:00119632

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1016/j.str.2021.03.003

UT WoS

000692378500008

Keywords in English

lectin; neutron diffraction; X-ray

Abstract

V originále

Carbohydrate-binding proteins from pathogenic bacteria and fungi have been shown to be implicated in various pathological processes, where they interact with glycans present on the surface of the host cells. These interactions are part of the initial processes of infection of the host and are very important to study at the atomic level. Here, we report the roomtemperature neutron structures of PLL lectin from Photorhabdus laumondii in its apo form and in complex with deuterated L-fucose, which is, to our knowledge, the first neutron structure of a carbohydrate-binding protein in complex with a fully deuterated carbohydrate ligand. A detailed structural analysis of the lectin-carbohydrate interactions provides information on the hydrogen bond network, the role of water molecules, and the extent of the CH-pi stacking interactions between fucose and the aromatic amino acids in the binding site.

Links

GA18-18964S, research and development project

Name: Lektiny a jejich úloha v interakci patogen/hostitel a buněčném rozpoznávání

Investor: Czech Science Foundation

Citovat

GAJDOŠ, Lukáš, Matthew P. BLAKELEY, Atul KUMAR, Michaela WIMMEROVÁ, Michaela HAERTLEIN, Trevor V. FORSYTH, Anne IMBERTY and Juliette M. DEVOS. Visualization of hydrogen atoms in a perdeuterated lectin-fucose complex reveals key details of protein-carbohydrate interactions. Structure. CAMBRIDGE: CELL PRESS, 2021, vol. 29, No 9, p. "1003"-"1013.e4", 15 pp. ISSN 0969-2126. Available from: https://dx.doi.org/10.1016/j.str.2021.03.003.

@article{1830859,
   author = {Gajdoš, Lukáš and Blakeley, Matthew P. and Kumar, Atul and Wimmerová, Michaela and Haertlein, Michaela and Forsyth, Trevor V. and Imberty, Anne and Devos, Juliette M.},
   article_location = {CAMBRIDGE},
   article_number = {9},
   doi = {http://dx.doi.org/10.1016/j.str.2021.03.003},
   keywords = {lectin; neutron diffraction; X-ray},
   language = {eng},
   issn = {0969-2126},
   journal = {Structure},
   title = {Visualization of hydrogen atoms in a perdeuterated lectin-fucose complex reveals key details of protein-carbohydrate interactions},
   url = {https://www.sciencedirect.com/science/article/pii/S0969212621000769?via%3Dihub},
   volume = {29},
   year = {2021}
}

TY  - JOUR
ID  - 1830859
AU  - Gajdoš, Lukáš - Blakeley, Matthew P. - Kumar, Atul - Wimmerová, Michaela - Haertlein, Michaela - Forsyth, Trevor V. - Imberty, Anne - Devos, Juliette M.
PY  - 2021
TI  - Visualization of hydrogen atoms in a perdeuterated lectin-fucose complex reveals key details of protein-carbohydrate interactions
JF  - Structure
VL  - 29
IS  - 9
SP  - "1003"-"1013.e4"
EP  - "1003"-"1013.e4"
PB  - CELL PRESS
SN  - 09692126
KW  - lectin
KW  - neutron diffraction
KW  - X-ray
UR  - https://www.sciencedirect.com/science/article/pii/S0969212621000769?via%3Dihub
N2  - Carbohydrate-binding proteins from pathogenic bacteria and fungi have been shown to be implicated in various pathological processes, where they interact with glycans present on the surface of the host cells. These interactions are part of the initial processes of infection of the host and are very important to study at the atomic level. Here, we report the roomtemperature neutron structures of PLL lectin from Photorhabdus laumondii in its apo form and in complex with deuterated L-fucose, which is, to our knowledge, the first neutron structure of a carbohydrate-binding protein in complex with a fully deuterated carbohydrate ligand. A detailed structural analysis of the lectin-carbohydrate interactions provides information on the hydrogen bond network, the role of water molecules, and the extent of the CH-pi stacking interactions between fucose and the aromatic amino acids in the binding site.
ER  -

GAJDOŠ, Lukáš, Matthew P. BLAKELEY, Atul KUMAR, Michaela WIMMEROVÁ, Michaela HAERTLEIN, Trevor V. FORSYTH, Anne IMBERTY and Juliette M. DEVOS. Visualization of hydrogen atoms in a perdeuterated lectin-fucose complex reveals key details of protein-carbohydrate interactions. \textit{Structure}. CAMBRIDGE: CELL PRESS, 2021, vol.~29, No~9, p.~''1003''-''1013.e4'', 15 pp. ISSN~0969-2126. Available from: https://dx.doi.org/10.1016/j.str.2021.03.003.

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