| GAJDOŠ, Lukáš, Matthew P. BLAKELEY, Atul KUMAR, Michaela WIMMEROVÁ, Michaela HAERTLEIN, Trevor V. FORSYTH, Anne IMBERTY and Juliette M. DEVOS. Visualization of hydrogen atoms in a perdeuterated lectin-fucose complex reveals key details of protein-carbohydrate interactions. Structure. CAMBRIDGE: CELL PRESS, 2021, vol. 29, No 9, p. "1003"-"1013.e4", 15 pp. ISSN 0969-2126. Available from: https://dx.doi.org/10.1016/j.str.2021.03.003. |
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@article{1830859,
author = {Gajdoš, Lukáš and Blakeley, Matthew P. and Kumar, Atul and Wimmerová, Michaela and Haertlein, Michaela and Forsyth, Trevor V. and Imberty, Anne and Devos, Juliette M.},
article_location = {CAMBRIDGE},
article_number = {9},
doi = {http://dx.doi.org/10.1016/j.str.2021.03.003},
keywords = {lectin; neutron diffraction; X-ray},
language = {eng},
issn = {0969-2126},
journal = {Structure},
title = {Visualization of hydrogen atoms in a perdeuterated lectin-fucose complex reveals key details of protein-carbohydrate interactions},
url = {https://www.sciencedirect.com/science/article/pii/S0969212621000769?via%3Dihub},
volume = {29},
year = {2021}
}
TY - JOUR
ID - 1830859
AU - Gajdoš, Lukáš - Blakeley, Matthew P. - Kumar, Atul - Wimmerová, Michaela - Haertlein, Michaela - Forsyth, Trevor V. - Imberty, Anne - Devos, Juliette M.
PY - 2021
TI - Visualization of hydrogen atoms in a perdeuterated lectin-fucose complex reveals key details of protein-carbohydrate interactions
JF - Structure
VL - 29
IS - 9
SP - "1003"-"1013.e4"
EP - "1003"-"1013.e4"
PB - CELL PRESS
SN - 09692126
KW - lectin
KW - neutron diffraction
KW - X-ray
UR - https://www.sciencedirect.com/science/article/pii/S0969212621000769?via%3Dihub
N2 - Carbohydrate-binding proteins from pathogenic bacteria and fungi have been shown to be implicated in various pathological processes, where they interact with glycans present on the surface of the host cells. These interactions are part of the initial processes of infection of the host and are very important to study at the atomic level. Here, we report the roomtemperature neutron structures of PLL lectin from Photorhabdus laumondii in its apo form and in complex with deuterated L-fucose, which is, to our knowledge, the first neutron structure of a carbohydrate-binding protein in complex with a fully deuterated carbohydrate ligand. A detailed structural analysis of the lectin-carbohydrate interactions provides information on the hydrogen bond network, the role of water molecules, and the extent of the CH-pi stacking interactions between fucose and the aromatic amino acids in the binding site.
ER -
GAJDOŠ, Lukáš, Matthew P. BLAKELEY, Atul KUMAR, Michaela WIMMEROVÁ, Michaela HAERTLEIN, Trevor V. FORSYTH, Anne IMBERTY and Juliette M. DEVOS. Visualization of hydrogen atoms in a perdeuterated lectin-fucose complex reveals key details of protein-carbohydrate interactions. \textit{Structure}. CAMBRIDGE: CELL PRESS, 2021, vol.~29, No~9, p.~''1003''-''1013.e4'', 15 pp. ISSN~0969-2126. Available from: https://dx.doi.org/10.1016/j.str.2021.03.003.
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