| PAVLÍK, Vojtěch, Veronika MACHALOVÁ, Martin ČEPA, Romana ŠÍNOVÁ, Barbora ŠAFRÁNKOVÁ, Jaromír KULHÁNEK, Tomáš DRMOTA, Lukáš KUBALA, Gloria HUERTA-ÁNGELES, Vladimír VELEBNÝ and Kristina NEŠPOROVÁ. Retinoic Acid Grafted to Hyaluronic Acid Activates Retinoid Gene Expression and Removes Cholesterol from Cellular Membranes. Biomolecules. MDPI, 2022, vol. 12, No 2, p. 1-14. ISSN 2218-273X. Available from: https://dx.doi.org/10.3390/biom12020200. |
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@article{1831253,
author = {Pavlík, Vojtěch and Machalová, Veronika and Čepa, Martin and Šínová, Romana and Šafránková, Barbora and Kulhánek, Jaromír and Drmota, Tomáš and Kubala, Lukáš and HuertaandÁngeles, Gloria and Velebný, Vladimír and Nešporová, Kristina},
article_number = {2},
doi = {http://dx.doi.org/10.3390/biom12020200},
keywords = {retinoic acid; hyaluronic acid; amphiphilic hyaluronan; nanocarrier; cholesterol; HyRetin; Delcore},
language = {eng},
issn = {2218-273X},
journal = {Biomolecules},
title = {Retinoic Acid Grafted to Hyaluronic Acid Activates Retinoid Gene Expression and Removes Cholesterol from Cellular Membranes},
url = {https://www.mdpi.com/2218-273X/12/2/200},
volume = {12},
year = {2022}
}
TY - JOUR
ID - 1831253
AU - Pavlík, Vojtěch - Machalová, Veronika - Čepa, Martin - Šínová, Romana - Šafránková, Barbora - Kulhánek, Jaromír - Drmota, Tomáš - Kubala, Lukáš - Huerta-Ángeles, Gloria - Velebný, Vladimír - Nešporová, Kristina
PY - 2022
TI - Retinoic Acid Grafted to Hyaluronic Acid Activates Retinoid Gene Expression and Removes Cholesterol from Cellular Membranes
JF - Biomolecules
VL - 12
IS - 2
SP - 1-14
EP - 1-14
PB - MDPI
SN - 2218273X
KW - retinoic acid
KW - hyaluronic acid
KW - amphiphilic hyaluronan
KW - nanocarrier
KW - cholesterol
KW - HyRetin
KW - Delcore
UR - https://www.mdpi.com/2218-273X/12/2/200
N2 - All-trans-retinoic acid (atRA) is a potent ligand that regulates gene expression and is used to treat several skin disorders. Hyaluronic acid (HA) was previously conjugated with atRA (HA-atRA) to obtain a novel amphiphilic compound. HA-atRA forms micelles that incorporate hydrophobic molecules and facilitate their transport through the skin. The aim of this study was to determine the influence of HA-atRA on gene expression in skin cells and to compare it with that of unbound atRA. Gene expression was investigated using microarrays and a luciferase system with a canonical atRA promoter. HA-atRA upregulated gene expression similarly to atRA. However, HA-atRA activated the expression of cholesterol metabolism genes, unlike atRA. Further investigation using HPLC and filipin III staining suggested that the treated cells induced cholesterol synthesis to replenish the cholesterol removed from the cells by HA-atRA. HA modified with oleate (HA-C18:1) removed cholesterol from the cells similarly to HA-atRA, suggesting that the cholesterol removal stemmed from the amphiphilic nature of the two derivatives. HA-atRA induces retinoid signaling. Thus, HA-atRA could be used to treat skin diseases, such as acne and psoriasis, where the combined action of atRA signaling and anti-inflammatory cholesterol removal may be potentially beneficial.
ER -
PAVLÍK, Vojtěch, Veronika MACHALOVÁ, Martin ČEPA, Romana ŠÍNOVÁ, Barbora ŠAFRÁNKOVÁ, Jaromír KULHÁNEK, Tomáš DRMOTA, Lukáš KUBALA, Gloria HUERTA-ÁNGELES, Vladimír VELEBNÝ and Kristina NEŠPOROVÁ. Retinoic Acid Grafted to Hyaluronic Acid Activates Retinoid Gene Expression and Removes Cholesterol from Cellular Membranes. \textit{Biomolecules}. MDPI, 2022, vol.~12, No~2, p.~1-14. ISSN~2218-273X. Available from: https://dx.doi.org/10.3390/biom12020200.
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