The Development of Dentin Microstructure Is Controlled by the
Type of Adjacent Epithelium

J 2022

The Development of Dentin Microstructure Is Controlled by the Type of Adjacent Epithelium

LAVICKÝ, Josef, Magdaléna KOLOUŠKOVÁ, David PROCHÁZKA, Vladislav RAKULTSEV, Marcos GONZÁLEZ LÓPEZ et. al.

Basic information

Original name

The Development of Dentin Microstructure Is Controlled by the Type of Adjacent Epithelium

Authors

LAVICKÝ, Josef (203 Czech Republic, belonging to the institution), Magdaléna KOLOUŠKOVÁ (203 Czech Republic, belonging to the institution), David PROCHÁZKA (203 Czech Republic), Vladislav RAKULTSEV (112 Belarus, belonging to the institution), Marcos GONZÁLEZ LÓPEZ (724 Spain, belonging to the institution), Klara STEKLIKOVA (203 Czech Republic), Martin BARTOŠ (203 Czech Republic), Anushree VIJAYKUMAR, Jozef KAISER (203 Czech Republic), Pavel POŘÍZKA (203 Czech Republic), Marie HOVORAKOVA (203 Czech Republic), Mina MINA and Jan KŘIVÁNEK (203 Czech Republic, guarantor, belonging to the institution)

Edition

Journal of Bone and Mineral Research, HOBOKEN, WILEY, 2022, 0884-0431

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30202 Endocrinology and metabolism

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 6.200

RIV identification code

RIV/00216224:14110/22:00119639

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1002/jbmr.4471

UT WoS

000729274700001

Keywords in English

TEETH; DENTIN; MICROSTRUCTURE; ODONTOBLAST; PROCESSES; ODONTOGENESIS; DENTINOGENESIS;; INCISOR; MOLAR; WNT SIGNALING; LIBS

Abstract

V originále

Considerable amount of research has been focused on dentin mineralization, odontoblast differentiation, and their application in dental tissue engineering. However, very little is known about the differential role of functionally and spatially distinct types of dental epithelium during odontoblast development. Here we show morphological and functional differences in dentin located in the crown and roots of mouse molar and analogous parts of continuously growing incisors. Using a reporter (DSPP-cerulean/DMP1-cherry) mouse strain and mice with ectopic enamel (Spry2+/−;Spry4−/−), we show that the different microstructure of dentin is initiated in the very beginning of dentin matrix production and is maintained throughout the whole duration of dentin growth. This phenomenon is regulated by the different inductive role of the adjacent epithelium. Thus, based on the type of interacting epithelium, we introduce more generalized terms for two distinct types of dentins: cementum versus enamel-facing dentin. In the odontoblasts, which produce enamel-facing dentin, we identified uniquely expressed genes (Dkk1, Wisp1, and Sall1) that were either absent or downregulated in odontoblasts, which form cementum-facing dentin. This suggests the potential role of Wnt signalling on the dentin structure patterning. Finally, we show the distribution of calcium and magnesium composition in the two developmentally different types of dentins by utilizing spatial element composition analysis (LIBS). Therefore, variations in dentin inner structure and element composition are the outcome of different developmental history initiated from the very beginning of tooth development. Taken together, our results elucidate the different effects of dental epithelium, during crown and root formation on adjacent odontoblasts and the possible role of Wnt signalling which together results in formation of dentin of different quality. © 2021 The Authors. Journal of Bone and Mineral Research published by Wiley Periodicals LLC on behalf of American Society for Bone and Mineral Research (ASBMR).

Links

GA21-04178S, research and development project

Name: Propojení funkce Sprouty s FGF a primárními ciliemi ve vývoji

Investor: Czech Science Foundation

LM2018129, research and development project

Name: Národní infrastruktura pro biologické a medicínské zobrazování Czech-BioImaging

Investor: Ministry of Education, Youth and Sports of the CR

MUNI/H/1615/2018, interní kód MU

Name: Understanding of tooth developmental trajectories as a novel approach for dental regeneration

Investor: Masaryk University, Individual High risk/high gain projects

MUNI/IGA/1532/2020, interní kód MU

Name: Investigation of the role of FoxD1+ stem cells during craniofacial development (Acronym: FoxD1 stem cells in craniofacial development)

Investor: Masaryk University

ROZV/23/LF17/2019, interní kód MU

Name: Vývoj a vnitřní uspořádání zubu

Investor: Ministry of Education, Youth and Sports of the CR, Internal development projects

Citovat

LAVICKÝ, Josef, Magdaléna KOLOUŠKOVÁ, David PROCHÁZKA, Vladislav RAKULTSEV, Marcos GONZÁLEZ LÓPEZ, Klara STEKLIKOVA, Martin BARTOŠ, Anushree VIJAYKUMAR, Jozef KAISER, Pavel POŘÍZKA, Marie HOVORAKOVA, Mina MINA and Jan KŘIVÁNEK. The Development of Dentin Microstructure Is Controlled by the Type of Adjacent Epithelium. Journal of Bone and Mineral Research. HOBOKEN: WILEY, 2022, vol. 37, No 2, p. 323-339. ISSN 0884-0431. Available from: https://dx.doi.org/10.1002/jbmr.4471.

@article{1832354,
   author = {Lavický, Josef and Koloušková, Magdaléna and Procházka, David and Rakultsev, Vladislav and González López, Marcos and Steklikova, Klara and Bartoš, Martin and Vijaykumar, Anushree and Kaiser, Jozef and Pořízka, Pavel and Hovorakova, Marie and Mina, Mina and Křivánek, Jan},
   article_location = {HOBOKEN},
   article_number = {2},
   doi = {http://dx.doi.org/10.1002/jbmr.4471},
   keywords = {TEETH; DENTIN; MICROSTRUCTURE; ODONTOBLAST; PROCESSES; ODONTOGENESIS; DENTINOGENESIS;; INCISOR; MOLAR; WNT SIGNALING; LIBS},
   language = {eng},
   issn = {0884-0431},
   journal = {Journal of Bone and Mineral Research},
   title = {The Development of Dentin Microstructure Is Controlled by the Type of Adjacent Epithelium},
   url = {https://asbmr.onlinelibrary.wiley.com/doi/10.1002/jbmr.4471},
   volume = {37},
   year = {2022}
}

TY  - JOUR
ID  - 1832354
AU  - Lavický, Josef - Koloušková, Magdaléna - Procházka, David - Rakultsev, Vladislav - González López, Marcos - Steklikova, Klara - Bartoš, Martin - Vijaykumar, Anushree - Kaiser, Jozef - Pořízka, Pavel - Hovorakova, Marie - Mina, Mina - Křivánek, Jan
PY  - 2022
TI  - The Development of Dentin Microstructure Is Controlled by the Type of Adjacent Epithelium
JF  - Journal of Bone and Mineral Research
VL  - 37
IS  - 2
SP  - 323-339
EP  - 323-339
PB  - WILEY
SN  - 08840431
KW  - TEETH
KW  - DENTIN
KW  - MICROSTRUCTURE
KW  - ODONTOBLAST
KW  - PROCESSES
KW  - ODONTOGENESIS
KW  - DENTINOGENESIS;
KW  - INCISOR
KW  - MOLAR
KW  - WNT SIGNALING
KW  - LIBS
UR  - https://asbmr.onlinelibrary.wiley.com/doi/10.1002/jbmr.4471
N2  - Considerable amount of research has been focused on dentin mineralization, odontoblast differentiation, and their application in dental tissue engineering. However, very little is known about the differential role of functionally and spatially distinct types of dental epithelium during odontoblast development. Here we show morphological and functional differences in dentin located in the crown and roots of mouse molar and analogous parts of continuously growing incisors. Using a reporter (DSPP-cerulean/DMP1-cherry) mouse strain and mice with ectopic enamel (Spry2+/−;Spry4−/−), we show that the different microstructure of dentin is initiated in the very beginning of dentin matrix production and is maintained throughout the whole duration of dentin growth. This phenomenon is regulated by the different inductive role of the adjacent epithelium. Thus, based on the type of interacting epithelium, we introduce more generalized terms for two distinct types of dentins: cementum versus enamel-facing dentin. In the odontoblasts, which produce enamel-facing dentin, we identified uniquely expressed genes (Dkk1, Wisp1, and Sall1) that were either absent or downregulated in odontoblasts, which form cementum-facing dentin. This suggests the potential role of Wnt signalling on the dentin structure patterning. Finally, we show the distribution of calcium and magnesium composition in the two developmentally different types of dentins by utilizing spatial element composition analysis (LIBS). Therefore, variations in dentin inner structure and element composition are the outcome of different developmental history initiated from the very beginning of tooth development. Taken together, our results elucidate the different effects of dental epithelium, during crown and root formation on adjacent odontoblasts and the possible role of Wnt signalling which together results in formation of dentin of different quality. © 2021 The Authors. Journal of Bone and Mineral Research published by Wiley Periodicals LLC on behalf of American Society for Bone and Mineral Research (ASBMR).
ER  -

LAVICKÝ, Josef, Magdaléna KOLOUŠKOVÁ, David PROCHÁZKA, Vladislav RAKULTSEV, Marcos GONZÁLEZ LÓPEZ, Klara STEKLIKOVA, Martin BARTOŠ, Anushree VIJAYKUMAR, Jozef KAISER, Pavel POŘÍZKA, Marie HOVORAKOVA, Mina MINA and Jan KŘIVÁNEK. The Development of Dentin Microstructure Is Controlled by the Type of Adjacent Epithelium. \textit{Journal of Bone and Mineral Research}. HOBOKEN: WILEY, 2022, vol.~37, No~2, p.~323-339. ISSN~0884-0431. Available from: https://dx.doi.org/10.1002/jbmr.4471.

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