Conductive Polymer PEDOT:PSS-Based Platform for Embryonic
Stem-Cell Differentiation

J 2022

Conductive Polymer PEDOT:PSS-Based Platform for Embryonic Stem-Cell Differentiation

VOJTKOVÁ, Eva, Jiří EHLICH, Stanislav STŘÍTESKÝ, Martin VALA, Martin WEITER et. al.

Basic information

Original name

Conductive Polymer PEDOT:PSS-Based Platform for Embryonic Stem-Cell Differentiation

Authors

VOJTKOVÁ, Eva (203 Czech Republic, belonging to the institution), Jiří EHLICH (203 Czech Republic), Stanislav STŘÍTESKÝ (203 Czech Republic), Martin VALA (203 Czech Republic), Martin WEITER (203 Czech Republic), Jiří PACHERNÍK (203 Czech Republic, belonging to the institution), Lukáš KUBALA (203 Czech Republic, belonging to the institution) and Jan VÍTEČEK (203 Czech Republic, guarantor)

Edition

International Journal of Molecular Sciences, MDPI, 2022, 1422-0067

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 5.600

RIV identification code

RIV/00216224:14310/22:00119641

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.3390/ijms23031107

UT WoS

000760579600001

Keywords in English

conductive polymer; PEDOT:PSS; screen print; embryonic stem cells; electrostimulation

Abstract

V originále

Organic semiconductors are constantly gaining interest in regenerative medicine. Their tunable physico-chemical properties, including electrical conductivity, are very promising for the control of stem-cell differentiation. However, their use for combined material-based and electrical stimulation remains largely underexplored. Therefore, we carried out a study on whether a platform based on the conductive polymer poly(3,4-ethylenedioxythiophene):polystyrene sulfonate (PEDOT:PSS) can be beneficial to the differentiation of mouse embryonic stem cells (mESCs). The platform was prepared using the layout of a standard 24-well cell-culture plate. Polyethylene naphthalate foil served as the substrate for the preparation of interdigitated gold electrodes by physical vapor deposition. The PEDOT:PSS pattern was fabricated by precise screen printing over the gold electrodes. The PEDOT:PSS platform was able to produce higher electrical current with the pulsed-direct-current (DC) electrostimulation mode (1 Hz, 200 mV/mm, 100 ms pulse duration) compared to plain gold electrodes. There was a dominant capacitive component. In proof-of-concept experiments, mESCs were able to respond to such electrostimulation by membrane depolarization and elevation of cytosolic calcium. Further, the PEDOT:PSS platform was able to upregulate cardiomyogenesis and potentially inhibit early neurogenesis per se with minor contribution of electrostimulation. Hence, the present work highlights the large potential of PEDOT:PSS in regenerative medicine.

Links

GA18-18235S, research and development project

Name: Nové možnosti v kardiomyogenní diferenciaci pluripotentních kmenových buněk (Acronym: KarPKB)

Investor: Czech Science Foundation

Citovat

VOJTKOVÁ, Eva, Jiří EHLICH, Stanislav STŘÍTESKÝ, Martin VALA, Martin WEITER, Jiří PACHERNÍK, Lukáš KUBALA and Jan VÍTEČEK. Conductive Polymer PEDOT:PSS-Based Platform for Embryonic Stem-Cell Differentiation. International Journal of Molecular Sciences. MDPI, 2022, vol. 23, No 3, p. 1-16. ISSN 1422-0067. Available from: https://dx.doi.org/10.3390/ijms23031107.

@article{1832630,
   author = {Vojtková, Eva and Ehlich, Jiří and Stříteský, Stanislav and Vala, Martin and Weiter, Martin and Pacherník, Jiří and Kubala, Lukáš and Víteček, Jan},
   article_number = {3},
   doi = {http://dx.doi.org/10.3390/ijms23031107},
   keywords = {conductive polymer; PEDOT:PSS; screen print; embryonic stem cells; electrostimulation},
   language = {eng},
   issn = {1422-0067},
   journal = {International Journal of Molecular Sciences},
   title = {Conductive Polymer PEDOT:PSS-Based Platform for Embryonic Stem-Cell Differentiation},
   url = {https://www.mdpi.com/1422-0067/23/3/1107},
   volume = {23},
   year = {2022}
}

TY  - JOUR
ID  - 1832630
AU  - Vojtková, Eva - Ehlich, Jiří - Stříteský, Stanislav - Vala, Martin - Weiter, Martin - Pacherník, Jiří - Kubala, Lukáš - Víteček, Jan
PY  - 2022
TI  - Conductive Polymer PEDOT:PSS-Based Platform for Embryonic Stem-Cell Differentiation
JF  - International Journal of Molecular Sciences
VL  - 23
IS  - 3
SP  - 1-16
EP  - 1-16
PB  - MDPI
SN  - 14220067
KW  - conductive polymer
KW  - PEDOT:PSS
KW  - screen print
KW  - embryonic stem cells
KW  - electrostimulation
UR  - https://www.mdpi.com/1422-0067/23/3/1107
N2  - Organic semiconductors are constantly gaining interest in regenerative medicine. Their tunable physico-chemical properties, including electrical conductivity, are very promising for the control of stem-cell differentiation. However, their use for combined material-based and electrical stimulation remains largely underexplored. Therefore, we carried out a study on whether a platform based on the conductive polymer poly(3,4-ethylenedioxythiophene):polystyrene sulfonate (PEDOT:PSS) can be beneficial to the differentiation of mouse embryonic stem cells (mESCs). The platform was prepared using the layout of a standard 24-well cell-culture plate. Polyethylene naphthalate foil served as the substrate for the preparation of interdigitated gold electrodes by physical vapor deposition. The PEDOT:PSS pattern was fabricated by precise screen printing over the gold electrodes. The PEDOT:PSS platform was able to produce higher electrical current with the pulsed-direct-current (DC) electrostimulation mode (1 Hz, 200 mV/mm, 100 ms pulse duration) compared to plain gold electrodes. There was a dominant capacitive component. In proof-of-concept experiments, mESCs were able to respond to such electrostimulation by membrane depolarization and elevation of cytosolic calcium. Further, the PEDOT:PSS platform was able to upregulate cardiomyogenesis and potentially inhibit early neurogenesis per se with minor contribution of electrostimulation. Hence, the present work highlights the large potential of PEDOT:PSS in regenerative medicine.
ER  -

VOJTKOVÁ, Eva, Jiří EHLICH, Stanislav STŘÍTESKÝ, Martin VALA, Martin WEITER, Jiří PACHERNÍK, Lukáš KUBALA and Jan VÍTEČEK. Conductive Polymer PEDOT:PSS-Based Platform for Embryonic Stem-Cell Differentiation. \textit{International Journal of Molecular Sciences}. MDPI, 2022, vol.~23, No~3, p.~1-16. ISSN~1422-0067. Available from: https://dx.doi.org/10.3390/ijms23031107.

Detailed Information on Publication Record