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@article{1832630, author = {Vojtková, Eva and Ehlich, Jiří and Stříteský, Stanislav and Vala, Martin and Weiter, Martin and Pacherník, Jiří and Kubala, Lukáš and Víteček, Jan}, article_number = {3}, doi = {http://dx.doi.org/10.3390/ijms23031107}, keywords = {conductive polymer; PEDOT:PSS; screen print; embryonic stem cells; electrostimulation}, language = {eng}, issn = {1422-0067}, journal = {International Journal of Molecular Sciences}, title = {Conductive Polymer PEDOT:PSS-Based Platform for Embryonic Stem-Cell Differentiation}, url = {https://www.mdpi.com/1422-0067/23/3/1107}, volume = {23}, year = {2022} }
TY - JOUR ID - 1832630 AU - Vojtková, Eva - Ehlich, Jiří - Stříteský, Stanislav - Vala, Martin - Weiter, Martin - Pacherník, Jiří - Kubala, Lukáš - Víteček, Jan PY - 2022 TI - Conductive Polymer PEDOT:PSS-Based Platform for Embryonic Stem-Cell Differentiation JF - International Journal of Molecular Sciences VL - 23 IS - 3 SP - 1-16 EP - 1-16 PB - MDPI SN - 14220067 KW - conductive polymer KW - PEDOT:PSS KW - screen print KW - embryonic stem cells KW - electrostimulation UR - https://www.mdpi.com/1422-0067/23/3/1107 N2 - Organic semiconductors are constantly gaining interest in regenerative medicine. Their tunable physico-chemical properties, including electrical conductivity, are very promising for the control of stem-cell differentiation. However, their use for combined material-based and electrical stimulation remains largely underexplored. Therefore, we carried out a study on whether a platform based on the conductive polymer poly(3,4-ethylenedioxythiophene):polystyrene sulfonate (PEDOT:PSS) can be beneficial to the differentiation of mouse embryonic stem cells (mESCs). The platform was prepared using the layout of a standard 24-well cell-culture plate. Polyethylene naphthalate foil served as the substrate for the preparation of interdigitated gold electrodes by physical vapor deposition. The PEDOT:PSS pattern was fabricated by precise screen printing over the gold electrodes. The PEDOT:PSS platform was able to produce higher electrical current with the pulsed-direct-current (DC) electrostimulation mode (1 Hz, 200 mV/mm, 100 ms pulse duration) compared to plain gold electrodes. There was a dominant capacitive component. In proof-of-concept experiments, mESCs were able to respond to such electrostimulation by membrane depolarization and elevation of cytosolic calcium. Further, the PEDOT:PSS platform was able to upregulate cardiomyogenesis and potentially inhibit early neurogenesis per se with minor contribution of electrostimulation. Hence, the present work highlights the large potential of PEDOT:PSS in regenerative medicine. ER -
VOJTKOVÁ, Eva, Jiří EHLICH, Stanislav STŘÍTESKÝ, Martin VALA, Martin WEITER, Jiří PACHERNÍK, Lukáš KUBALA and Jan VÍTEČEK. Conductive Polymer PEDOT:PSS-Based Platform for Embryonic Stem-Cell Differentiation. \textit{International Journal of Molecular Sciences}. MDPI, 2022, vol.~23, No~3, p.~1-16. ISSN~1422-0067. Available from: https://dx.doi.org/10.3390/ijms23031107.
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