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@article{1832630,
author = {Vojtková, Eva and Ehlich, Jiří and Stříteský, Stanislav and Vala, Martin and Weiter, Martin and Pacherník, Jiří and Kubala, Lukáš and Víteček, Jan},
article_number = {3},
doi = {http://dx.doi.org/10.3390/ijms23031107},
keywords = {conductive polymer; PEDOT:PSS; screen print; embryonic stem cells; electrostimulation},
language = {eng},
issn = {1422-0067},
journal = {International Journal of Molecular Sciences},
title = {Conductive Polymer PEDOT:PSS-Based Platform for Embryonic Stem-Cell Differentiation},
url = {https://www.mdpi.com/1422-0067/23/3/1107},
volume = {23},
year = {2022}
}
TY - JOUR
ID - 1832630
AU - Vojtková, Eva - Ehlich, Jiří - Stříteský, Stanislav - Vala, Martin - Weiter, Martin - Pacherník, Jiří - Kubala, Lukáš - Víteček, Jan
PY - 2022
TI - Conductive Polymer PEDOT:PSS-Based Platform for Embryonic Stem-Cell Differentiation
JF - International Journal of Molecular Sciences
VL - 23
IS - 3
SP - 1-16
EP - 1-16
PB - MDPI
SN - 14220067
KW - conductive polymer
KW - PEDOT:PSS
KW - screen print
KW - embryonic stem cells
KW - electrostimulation
UR - https://www.mdpi.com/1422-0067/23/3/1107
N2 - Organic semiconductors are constantly gaining interest in regenerative medicine. Their tunable physico-chemical properties, including electrical conductivity, are very promising for the control of stem-cell differentiation. However, their use for combined material-based and electrical stimulation remains largely underexplored. Therefore, we carried out a study on whether a platform based on the conductive polymer poly(3,4-ethylenedioxythiophene):polystyrene sulfonate (PEDOT:PSS) can be beneficial to the differentiation of mouse embryonic stem cells (mESCs). The platform was prepared using the layout of a standard 24-well cell-culture plate. Polyethylene naphthalate foil served as the substrate for the preparation of interdigitated gold electrodes by physical vapor deposition. The PEDOT:PSS pattern was fabricated by precise screen printing over the gold electrodes. The PEDOT:PSS platform was able to produce higher electrical current with the pulsed-direct-current (DC) electrostimulation mode (1 Hz, 200 mV/mm, 100 ms pulse duration) compared to plain gold electrodes. There was a dominant capacitive component. In proof-of-concept experiments, mESCs were able to respond to such electrostimulation by membrane depolarization and elevation of cytosolic calcium. Further, the PEDOT:PSS platform was able to upregulate cardiomyogenesis and potentially inhibit early neurogenesis per se with minor contribution of electrostimulation. Hence, the present work highlights the large potential of PEDOT:PSS in regenerative medicine.
ER -
VOJTKOVÁ, Eva, Jiří EHLICH, Stanislav STŘÍTESKÝ, Martin VALA, Martin WEITER, Jiří PACHERNÍK, Lukáš KUBALA and Jan VÍTEČEK. Conductive Polymer PEDOT:PSS-Based Platform for Embryonic Stem-Cell Differentiation. \textit{International Journal of Molecular Sciences}. MDPI, 2022, vol.~23, No~3, p.~1-16. ISSN~1422-0067. Available from: https://dx.doi.org/10.3390/ijms23031107.
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