Changes of peptidase profile during Fasciola hepatica
embryonation

a 2021

Changes of peptidase profile during Fasciola hepatica embryonation

ILGOVÁ, Jana, Lucie ŠKORPÍKOVÁ, Jiří VOREL, Pavel ROUDNICKÝ, Martin HORN et. al.

Basic information

Original name

Changes of peptidase profile during Fasciola hepatica embryonation

Name in Czech

Změny peptidázového profily v průběhu embryonace Fasciola hepatica

Authors

ILGOVÁ, Jana (703 Slovakia, guarantor, belonging to the institution), Lucie ŠKORPÍKOVÁ (203 Czech Republic, belonging to the institution), Jiří VOREL (203 Czech Republic, belonging to the institution), Pavel ROUDNICKÝ (203 Czech Republic), Martin HORN (203 Czech Republic) and Martin KAŠNÝ (203 Czech Republic, belonging to the institution)

Edition

XXVI. Helmintologické dny, 2021

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/21:00119646

Organization unit

Faculty of Science

ISBN

978-80-906644-9-4

Keywords in English

Fasciola hepatica; eggs;embryonation; peptidases; omics

Změněno: 14/2/2022 22:13, Mgr. et Mgr. Jana Ilgová, Ph.D.

Abstract

V originále

Fasciola hepatica is a widespread foodborne trematode that parasitizes a broad range of mammals, including humans. Adults of this cosmopolitan species lay their propagation stages - unsegmented operculated eggs, into the bile duct of their mammalian host. Crucial aspects of F. hepatica life cycle, such as migration, reproduction and immune evasion, are directly dependent on the activity of proteolytic enzymes, also known as peptidases. Expression of these molecules have been intensely studied in metacercariae, newly excysted juveniles and adults, however embryonic stages of F. hepatica, have not yielded much scientific attention in this context. We used combined transcriptomic/proteomic approach which led to the identification of expressed and translated peptidases in developing eggs of different age. Additionally, activity profiling with the substrate library and selective inhibitors identified presence of different peptidase classes in egg extracts. Our results indicate that F. hepatica egg transcriptome remains mostly constant, while the proteome is being considerably adjusted throughout the embryonation. Threonine catalytic subunits of proteasome are constantly produced, while the cysteine peptidases prevailing in freshly laid eggs are supplemented by aspartic peptidases and metallopeptidases in the later stages of egg development.

Links

GA19-17269S, research and development project

Name: Proteolýza ve vejcích parazitických červů: úloha v patologii a její regulace (Acronym: Proteolýza vajec červů)

Investor: Czech Science Foundation

Citovat

ILGOVÁ, Jana, Lucie ŠKORPÍKOVÁ, Jiří VOREL, Pavel ROUDNICKÝ, Martin HORN and Martin KAŠNÝ. Changes of peptidase profile during Fasciola hepatica embryonation. In XXVI. Helmintologické dny. 2021. ISBN 978-80-906644-9-4.

@proceedings{1834178,
   author = {Ilgová, Jana and Škorpíková, Lucie and Vorel, Jiří and Roudnický, Pavel and Horn, Martin and Kašný, Martin},
   booktitle = {XXVI. Helmintologické dny},
   keywords = {Fasciola hepatica; eggs;embryonation; peptidases; omics},
   language = {eng},
   isbn = {978-80-906644-9-4},
   title = {Changes of peptidase profile during Fasciola hepatica embryonation},
   year = {2021}
}

TY  - CONF
ID  - 1834178
AU  - Ilgová, Jana - Škorpíková, Lucie - Vorel, Jiří - Roudnický, Pavel - Horn, Martin - Kašný, Martin
PY  - 2021
TI  - Changes of peptidase profile during Fasciola hepatica embryonation
SN  - 9788090664494
KW  - Fasciola hepatica
KW  - eggs;embryonation
KW  - peptidases
KW  - omics
N2  - Fasciola hepatica is a widespread foodborne trematode that parasitizes a broad range of mammals, including humans. Adults of this cosmopolitan species lay their propagation stages - unsegmented operculated eggs, into the bile duct of their mammalian host. Crucial aspects of F. hepatica life cycle, such as migration, reproduction and immune evasion, are directly dependent on the activity of proteolytic enzymes, also known as peptidases. Expression of these molecules have been intensely studied in metacercariae, newly excysted juveniles and adults, however embryonic stages of F. hepatica, have not yielded much scientific attention in this context. We used combined transcriptomic/proteomic approach which led to the identification of expressed and translated peptidases in developing eggs of different age. Additionally, activity profiling with the substrate library and selective inhibitors identified presence of different peptidase classes in egg extracts. Our results indicate that F. hepatica egg transcriptome remains mostly constant, while the proteome is being considerably adjusted throughout the embryonation. Threonine catalytic subunits of proteasome are constantly produced, while the cysteine peptidases prevailing in freshly laid eggs are supplemented by aspartic peptidases and metallopeptidases in the later stages of egg development.
ER  -

ILGOVÁ, Jana, Lucie ŠKORPÍKOVÁ, Jiří VOREL, Pavel ROUDNICKÝ, Martin HORN and Martin KAŠNÝ. Changes of peptidase profile during Fasciola hepatica embryonation. In \textit{XXVI. Helmintologické dny}. 2021. ISBN~978-80-906644-9-4.

Detailed Information on Publication Record