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@article{1834297,
author = {Sucha, Rita and Kubickova, Martina and Cervenka, Jakub and HruskaandPlochan, Marian and Bohačiaková, Dáša and Kepkova Vodickova, Katerina and Novakova, Tereza and Budkova, Katerina and Susor, Andrej and Marsala, Martin and Motlik, Jan and Kovarova, Hana and Vodicka, Petr},
article_location = {CAMBRIDGE},
article_number = {8},
doi = {http://dx.doi.org/10.1242/bio.058727},
keywords = {Neural stem cell; Neural differentiation; Selected reaction monitoring; Mass spectrometry; Cell line characterization; Protein marker},
language = {eng},
issn = {2046-6390},
journal = {Biology Open},
title = {Targeted mass spectrometry for monitoring of neural differentiation},
url = {https://journals.biologists.com/bio/article/10/8/bio058727/271174/Targeted-mass-spectrometry-for-monitoring-of},
volume = {10},
year = {2021}
}
TY - JOUR
ID - 1834297
AU - Sucha, Rita - Kubickova, Martina - Cervenka, Jakub - Hruska-Plochan, Marian - Bohačiaková, Dáša - Kepkova Vodickova, Katerina - Novakova, Tereza - Budkova, Katerina - Susor, Andrej - Marsala, Martin - Motlik, Jan - Kovarova, Hana - Vodicka, Petr
PY - 2021
TI - Targeted mass spectrometry for monitoring of neural differentiation
JF - Biology Open
VL - 10
IS - 8
SP - 1-13
EP - 1-13
PB - COMPANY BIOLOGISTS LTD
SN - 20466390
KW - Neural stem cell
KW - Neural differentiation
KW - Selected reaction monitoring
KW - Mass spectrometry
KW - Cell line characterization
KW - Protein marker
UR - https://journals.biologists.com/bio/article/10/8/bio058727/271174/Targeted-mass-spectrometry-for-monitoring-of
N2 - Human multipotent neural stem cells could effectively be used for the treatment of a variety of neurological disorders. However, a defining signature of neural stem cell lines that would be expandable, non-tumorigenic, and differentiate into desirable neuronal/glial phenotype after in vivo grafting is not yet defined. Employing a mass spectrometry approach, based on selected reaction monitoring, we tested a panel of well-described culture conditions, and measured levels of protein markers routinely used to probe neural differentiation, i.e. POU5F1 (OCT4), SOX2, NES, DCX, TUBB3, MAP2, S100B, GFAP, GALC, and OLIG1. Our multiplexed assay enabled us to simultaneously identify the presence of pluripotent, multipotent, and lineage committed neural cells, thus representing a powerful tool to optimize novel and highly specific propagation and differentiation protocols. The multiplexing capacity of this method permits the addition of other newly identified cell type-specific markers to further increase the specificity and quantitative accuracy in detecting targeted cell populations. Such an expandable assay may gain the advantage over traditional antibody-based assays, and represents a method of choice for quality control of neural stem cell lines intended for clinical use.
ER -
SUCHA, Rita, Martina KUBICKOVA, Jakub CERVENKA, Marian HRUSKA-PLOCHAN, Dáša BOHAČIAKOVÁ, Katerina KEPKOVA VODICKOVA, Tereza NOVAKOVA, Katerina BUDKOVA, Andrej SUSOR, Martin MARSALA, Jan MOTLIK, Hana KOVAROVA and Petr VODICKA. Targeted mass spectrometry for monitoring of neural differentiation. \textit{Biology Open}. CAMBRIDGE: COMPANY BIOLOGISTS LTD, 2021, vol.~10, No~8, p.~1-13. ISSN~2046-6390. Available from: https://dx.doi.org/10.1242/bio.058727.
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