Genetic diversity of Leptospira isolates in Lao PDR and genome
analysis of an outbreak strain

J 2021

Genetic diversity of Leptospira isolates in Lao PDR and genome analysis of an outbreak strain

GRILLOVÁ, Linda, M. T. ROBINSON, A. CHANTHONGTHIP, A. T. VINCENT, C. NIEVES et. al.

Basic information

Original name

Genetic diversity of Leptospira isolates in Lao PDR and genome analysis of an outbreak strain

Authors

GRILLOVÁ, Linda (203 Czech Republic, belonging to the institution), M. T. ROBINSON, A. CHANTHONGTHIP, A. T. VINCENT, C. NIEVES, J. OPPELT, J. F. MARIET, C. LORIOUX, M. VONGSOUVATH, M. MAYXAY, O. PHONEMEEXAY, S. RATTANAVONG, K. PHOMMASONE, A. DOUANGNOUVONG, David ŠMAJS (203 Czech Republic, belonging to the institution), F. J. VEYRIER, P. N. NEWTON and M. PICARDEAU (guarantor)

Edition

PLoS neglected tropical diseases, San Francisco, Public Library of Science, 2021, 1935-2735

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30303 Infectious Diseases

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.781

RIV identification code

RIV/00216224:14110/21:00124154

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1371/journal.pntd.0010076

UT WoS

000739688900002

Keywords in English

Leptospira; Lao PDR; outbreak strain; genome analysis

Abstract

V originále

BackgroundAlthough Southeast Asia is one of the most leptospirosis afflicted regions, little is known about the diversity and molecular epidemiology of the causative agents of this widespread and emerging zoonotic disease. Methodology/Principal findingsWe used whole genome sequencing to examine genetic variation in 75 Leptospira strains isolated from patients in the Lao PDR (Laos) between 2006 and 2017.Eleven serogroups from 4 Leptospira species and 43 cgMLST-defined clonal groups (CGs) were identified. The most prevalent CG was CG272 (n = 18, 26.8%), composed of L. interrogans serogroup Autumnalis isolates. This genotype was recovered throughout the 12-year period and was associated with deaths, and with a large outbreak in neighbouring Thailand. Genome analysis reveals that the CG272 strains form a highly clonal group of strains that have, for yet unknown reasons, recently spread in Laos and Thailand. Additionally, accessory genes clearly discriminate CG272 strains from the other Leptospira strains. Conclusions/SignificanceThe present study reveals a high diversity of Leptospira genotypes in Laos, thus extending our current knowledge of the pan- and core-genomes of these life-threatening pathogens. Our results demonstrate that the CG272 strains belong to a unique clonal group, which probably evolved through clonal expansion following niche adaptation. Additional epidemiological studies are required to better evaluate the spread of this genotype in Southeast Asia. To further investigate the key factors driving the virulence and spread of these pathogens, more intense genomic surveillance is needed, combining detailed clinical and epidemiological data. Author summaryPathogenic Leptospira are the causative agents for leptospirosis, a neglected and emerging zoonosis occurring worldwide. In this study, we investigated the genetic diversity of Leptospira strains isolated from patients over a 12-year period in Lao PDR. Our genome analysis revealed a high diversity of Leptospira genotypes in this endemic country for leptospirosis, including a group of clonal strains which was responsible for a large outbreak in neighboring Thailand. Further progress in our understanding of the epidemiology of Leptospira circulating genotypes should contribute to the implementation of prevention and intervention measures to reduce the risk of leptospirosis transmission.

Links

LM2018140, research and development project

Name: e-Infrastruktura CZ (Acronym: e-INFRA CZ)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

GRILLOVÁ, Linda, M. T. ROBINSON, A. CHANTHONGTHIP, A. T. VINCENT, C. NIEVES, J. OPPELT, J. F. MARIET, C. LORIOUX, M. VONGSOUVATH, M. MAYXAY, O. PHONEMEEXAY, S. RATTANAVONG, K. PHOMMASONE, A. DOUANGNOUVONG, David ŠMAJS, F. J. VEYRIER, P. N. NEWTON and M. PICARDEAU. Genetic diversity of Leptospira isolates in Lao PDR and genome analysis of an outbreak strain. PLoS neglected tropical diseases. San Francisco: Public Library of Science, 2021, vol. 15, No 12, p. 1-19. ISSN 1935-2735. Available from: https://dx.doi.org/10.1371/journal.pntd.0010076.

@article{1834308,
   author = {Grillová, Linda and Robinson, M. T. and Chanthongthip, A. and Vincent, A. T. and Nieves, C. and Oppelt, J. and Mariet, J. F. and Lorioux, C. and Vongsouvath, M. and Mayxay, M. and Phonemeexay, O. and Rattanavong, S. and Phommasone, K. and Douangnouvong, A. and Šmajs, David and Veyrier, F. J. and Newton, P. N. and Picardeau, M.},
   article_location = {San Francisco},
   article_number = {12},
   doi = {http://dx.doi.org/10.1371/journal.pntd.0010076},
   keywords = {Leptospira; Lao PDR; outbreak strain; genome analysis},
   language = {eng},
   issn = {1935-2735},
   journal = {PLoS neglected tropical diseases},
   title = {Genetic diversity of Leptospira isolates in Lao PDR and genome analysis of an outbreak strain},
   url = {https://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0010076},
   volume = {15},
   year = {2021}
}

TY  - JOUR
ID  - 1834308
AU  - Grillová, Linda - Robinson, M. T. - Chanthongthip, A. - Vincent, A. T. - Nieves, C. - Oppelt, J. - Mariet, J. F. - Lorioux, C. - Vongsouvath, M. - Mayxay, M. - Phonemeexay, O. - Rattanavong, S. - Phommasone, K. - Douangnouvong, A. - Šmajs, David - Veyrier, F. J. - Newton, P. N. - Picardeau, M.
PY  - 2021
TI  - Genetic diversity of Leptospira isolates in Lao PDR and genome analysis of an outbreak strain
JF  - PLoS neglected tropical diseases
VL  - 15
IS  - 12
SP  - 1-19
EP  - 1-19
PB  - Public Library of Science
SN  - 19352735
KW  - Leptospira
KW  - Lao PDR
KW  - outbreak strain
KW  - genome analysis
UR  - https://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0010076
N2  - BackgroundAlthough Southeast Asia is one of the most leptospirosis afflicted regions, little is known about the diversity and molecular epidemiology of the causative agents of this widespread and emerging zoonotic disease. Methodology/Principal findingsWe used whole genome sequencing to examine genetic variation in 75 Leptospira strains isolated from patients in the Lao PDR (Laos) between 2006 and 2017.Eleven serogroups from 4 Leptospira species and 43 cgMLST-defined clonal groups (CGs) were identified. The most prevalent CG was CG272 (n = 18, 26.8%), composed of L. interrogans serogroup Autumnalis isolates. This genotype was recovered throughout the 12-year period and was associated with deaths, and with a large outbreak in neighbouring Thailand. Genome analysis reveals that the CG272 strains form a highly clonal group of strains that have, for yet unknown reasons, recently spread in Laos and Thailand. Additionally, accessory genes clearly discriminate CG272 strains from the other Leptospira strains. Conclusions/SignificanceThe present study reveals a high diversity of Leptospira genotypes in Laos, thus extending our current knowledge of the pan- and core-genomes of these life-threatening pathogens. Our results demonstrate that the CG272 strains belong to a unique clonal group, which probably evolved through clonal expansion following niche adaptation. Additional epidemiological studies are required to better evaluate the spread of this genotype in Southeast Asia. To further investigate the key factors driving the virulence and spread of these pathogens, more intense genomic surveillance is needed, combining detailed clinical and epidemiological data. Author summaryPathogenic Leptospira are the causative agents for leptospirosis, a neglected and emerging zoonosis occurring worldwide. In this study, we investigated the genetic diversity of Leptospira strains isolated from patients over a 12-year period in Lao PDR. Our genome analysis revealed a high diversity of Leptospira genotypes in this endemic country for leptospirosis, including a group of clonal strains which was responsible for a large outbreak in neighboring Thailand. Further progress in our understanding of the epidemiology of Leptospira circulating genotypes should contribute to the implementation of prevention and intervention measures to reduce the risk of leptospirosis transmission.
ER  -

GRILLOVÁ, Linda, M. T. ROBINSON, A. CHANTHONGTHIP, A. T. VINCENT, C. NIEVES, J. OPPELT, J. F. MARIET, C. LORIOUX, M. VONGSOUVATH, M. MAYXAY, O. PHONEMEEXAY, S. RATTANAVONG, K. PHOMMASONE, A. DOUANGNOUVONG, David ŠMAJS, F. J. VEYRIER, P. N. NEWTON and M. PICARDEAU. Genetic diversity of Leptospira isolates in Lao PDR and genome analysis of an outbreak strain. \textit{PLoS neglected tropical diseases}. San Francisco: Public Library of Science, 2021, vol.~15, No~12, p.~1-19. ISSN~1935-2735. Available from: https://dx.doi.org/10.1371/journal.pntd.0010076.

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