Variety of size and form of GRM2 bacterial microcompartment
particles

J 2021

Variety of size and form of GRM2 bacterial microcompartment particles

CESLE, E.E., Anatolij FILIMONĚNKO, K. TARS and G. KALNINS

Basic information

Original name

Variety of size and form of GRM2 bacterial microcompartment particles

Authors

CESLE, E.E., Anatolij FILIMONĚNKO (203 Czech Republic, guarantor, belonging to the institution), K. TARS and G. KALNINS

Edition

Protein Science, HOBOKEN, JOHN WILEY & SONS INC, 2021, 0961-8368

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 6.993

RIV identification code

RIV/00216224:14740/21:00124188

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1002/pro.4069

UT WoS

000635930300001

Keywords in English

bacterial microcompartments; cryo‐ EM; GRM2; Klebsiella pneumoniae

Abstract

V originále

Bacterial microcompartments (BMCs) are bacterial organelles involved in enzymatic processes, such as carbon fixation, choline, ethanolamine and propanediol degradation, and others. Formed of a semi-permeable protein shell and an enzymatic core, they can enhance enzyme performance and protect the cell from harmful intermediates. With the ability to encapsulate non-native enzymes, BMCs show high potential for applied use. For this goal, a detailed look into shell form variability is significant to predict shell adaptability. Here we present four novel 3D cryo-EM maps of recombinant Klebsiella pneumoniae GRM2 BMC shell particles with the resolution in range of 9 to 22 angstrom and nine novel 2D classes corresponding to discrete BMC shell forms. These structures reveal icosahedral, elongated, oblate, multi-layered and polyhedral traits of BMCs, indicating considerable variation in size and form as well as adaptability during shell formation processes.

Links

LM2018127, research and development project

Name: Česká infrastruktura pro integrativní strukturní biologii (Acronym: CIISB)

Investor: Ministry of Education, Youth and Sports of the CR

90127, large research infrastructures

Name: CIISB II

Citovat

CESLE, E.E., Anatolij FILIMONĚNKO, K. TARS and G. KALNINS. Variety of size and form of GRM2 bacterial microcompartment particles. Protein Science. HOBOKEN: JOHN WILEY & SONS INC, 2021, vol. 30, No 5, p. 1035-1043. ISSN 0961-8368. Available from: https://dx.doi.org/10.1002/pro.4069.

@article{1834740,
   author = {Cesle, E.E. and Filimoněnko, Anatolij and Tars, K. and Kalnins, G.},
   article_location = {HOBOKEN},
   article_number = {5},
   doi = {http://dx.doi.org/10.1002/pro.4069},
   keywords = {bacterial microcompartments; cryo‐ EM; GRM2; Klebsiella pneumoniae},
   language = {eng},
   issn = {0961-8368},
   journal = {Protein Science},
   title = {Variety of size and form of GRM2 bacterial microcompartment particles},
   url = {https://onlinelibrary.wiley.com/doi/10.1002/pro.4069},
   volume = {30},
   year = {2021}
}

TY  - JOUR
ID  - 1834740
AU  - Cesle, E.E. - Filimoněnko, Anatolij - Tars, K. - Kalnins, G.
PY  - 2021
TI  - Variety of size and form of GRM2 bacterial microcompartment particles
JF  - Protein Science
VL  - 30
IS  - 5
SP  - 1035-1043
EP  - 1035-1043
PB  - JOHN WILEY & SONS INC
SN  - 09618368
KW  - bacterial microcompartments
KW  - cryo‐ EM
KW  - GRM2
KW  - Klebsiella pneumoniae
UR  - https://onlinelibrary.wiley.com/doi/10.1002/pro.4069
N2  - Bacterial microcompartments (BMCs) are bacterial organelles involved in enzymatic processes, such as carbon fixation, choline, ethanolamine and propanediol degradation, and others. Formed of a semi-permeable protein shell and an enzymatic core, they can enhance enzyme performance and protect the cell from harmful intermediates. With the ability to encapsulate non-native enzymes, BMCs show high potential for applied use. For this goal, a detailed look into shell form variability is significant to predict shell adaptability. Here we present four novel 3D cryo-EM maps of recombinant Klebsiella pneumoniae GRM2 BMC shell particles with the resolution in range of 9 to 22 angstrom and nine novel 2D classes corresponding to discrete BMC shell forms. These structures reveal icosahedral, elongated, oblate, multi-layered and polyhedral traits of BMCs, indicating considerable variation in size and form as well as adaptability during shell formation processes.
ER  -

CESLE, E.E., Anatolij FILIMONĚNKO, K. TARS and G. KALNINS. Variety of size and form of GRM2 bacterial microcompartment particles. \textit{Protein Science}. HOBOKEN: JOHN WILEY \&{}amp; SONS INC, 2021, vol.~30, No~5, p.~1035-1043. ISSN~0961-8368. Available from: https://dx.doi.org/10.1002/pro.4069.

Detailed Information on Publication Record