Variety of size and form of GRM2 bacterial microcompartment particles

CESLE, E.E., Anatolij FILIMONĚNKO, K. TARS and G. KALNINS. Variety of size and form of GRM2 bacterial microcompartment particles. Protein Science. HOBOKEN: JOHN WILEY & SONS INC, 2021, vol. 30, No 5, p. 1035-1043. ISSN 0961-8368. Available from: https://dx.doi.org/10.1002/pro.4069.

Basic information

• Original name: Variety of size and form of GRM2 bacterial microcompartment particles
• Authors: CESLE, E.E., Anatolij FILIMONĚNKO (203 Czech Republic, guarantor, belonging to the institution), K. TARS and G. KALNINS.
• Edition: Protein Science, HOBOKEN, JOHN WILEY & SONS INC, 2021, 0961-8368.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10608 Biochemistry and molecular biology
• Country of publisher: Netherlands
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Impact factor: Impact factor: 6.993
• RIV identification code: RIV/00216224:14740/21:00124188
• Organization unit: Central European Institute of Technology
• Doi: http://dx.doi.org/10.1002/pro.4069
• UT WoS: 000635930300001
• Keywords in English: bacterial microcompartments; cryo‐ EM; GRM2; Klebsiella pneumoniae
• Tags: CF CRYO, rivok
• Tags: International impact, Reviewed

Abstract

Bacterial microcompartments (BMCs) are bacterial organelles involved in enzymatic processes, such as carbon fixation, choline, ethanolamine and propanediol degradation, and others. Formed of a semi-permeable protein shell and an enzymatic core, they can enhance enzyme performance and protect the cell from harmful intermediates. With the ability to encapsulate non-native enzymes, BMCs show high potential for applied use. For this goal, a detailed look into shell form variability is significant to predict shell adaptability. Here we present four novel 3D cryo-EM maps of recombinant Klebsiella pneumoniae GRM2 BMC shell particles with the resolution in range of 9 to 22 angstrom and nine novel 2D classes corresponding to discrete BMC shell forms. These structures reveal icosahedral, elongated, oblate, multi-layered and polyhedral traits of BMCs, indicating considerable variation in size and form as well as adaptability during shell formation processes.

Links

• LM2018127, research and development project: Name: Česká infrastruktura pro integrativní strukturní biologii (Acronym: CIISB)

Investor: Ministry of Education, Youth and Sports of the CR