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@article{1834920,
author = {Levien, Monique and Farka, Zdeněk and Pastucha, Matěj and Skládal, Petr and Nasri, Zahra and Weltmann, KlausandDieter and Fricke, Katja},
article_number = {May},
doi = {http://dx.doi.org/10.1016/j.apsusc.2022.152511},
keywords = {Hydrogel coating; Functional surface; Atmospheric pressure plasma polymerization; Electrochemical biosensor; Amperometry; Glucose oxidase; Acetylcholinesterase},
language = {eng},
issn = {0169-4332},
journal = {Applied Surface Science},
title = {Functional plasma-polymerized hydrogel coatings for electrochemical biosensing},
url = {https://www.sciencedirect.com/science/article/abs/pii/S0169433222000964},
volume = {584},
year = {2022}
}
TY - JOUR
ID - 1834920
AU - Levien, Monique - Farka, Zdeněk - Pastucha, Matěj - Skládal, Petr - Nasri, Zahra - Weltmann, Klaus-Dieter - Fricke, Katja
PY - 2022
TI - Functional plasma-polymerized hydrogel coatings for electrochemical biosensing
JF - Applied Surface Science
VL - 584
IS - May
SP - 1-8
EP - 1-8
PB - Elsevier
SN - 01694332
KW - Hydrogel coating
KW - Functional surface
KW - Atmospheric pressure plasma polymerization
KW - Electrochemical biosensor
KW - Amperometry
KW - Glucose oxidase
KW - Acetylcholinesterase
UR - https://www.sciencedirect.com/science/article/abs/pii/S0169433222000964
N2 - Acrylate-based hydrogels with multifunctional properties have proven to be suitable candidates for the development of sensor systems. They gained popularity especially in combination with bioelectronics, as there is a need to understand and control the interactions of bionic devices with the human body and other environments. In this study, we present results on the biointeraction capability of plasma-polymerized (pp) hydrogels made of hydroxyethyl methacrylate (HEMA) and 2-(diethylamino)ethyl methacrylate (DEAEMA) mixtures on gold screen-printed electrodes (SPE). The hydrogels were generated by an atmospheric pressure plasma jet, and their chemical composition was characterized via FT-IR. The FT-IR analysis revealed several functional groups suitable for biomolecule immobilization, whereas the amount of -C-N, –OH, and -C-O-C groups differs depending on the mixture ratios. The pp HEMA:DEAEMA (HD) hydrogel coatings provide alternative interfacing materials for electrochemical biosensing. The enzymes glucose oxidase (GOx) and acetylcholinesterase (AChE) were coupled to the hydrogel-based surfaces, and the effects of the mixture ratios on the biomolecule immobilization were investigated. It is possible to address different functional groups of the mixtures with different immobilization strategies; thus, the sensor response can be optimized. Finally, glucose as GOx substrate and eserine as AChE inhibitor were detected by amperometry to demonstrate the practical biosensing applicability of the coatings.
ER -
LEVIEN, Monique, Zdeněk FARKA, Matěj PASTUCHA, Petr SKLÁDAL, Zahra NASRI, Klaus-Dieter WELTMANN and Katja FRICKE. Functional plasma-polymerized hydrogel coatings for electrochemical biosensing. \textit{Applied Surface Science}. Elsevier, 2022, vol.~584, May, p.~1-8. ISSN~0169-4332. Available from: https://dx.doi.org/10.1016/j.apsusc.2022.152511.
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