In Vitro Characterization of Sumoylation of HR Proteins

C 2021

In Vitro Characterization of Sumoylation of HR Proteins

ALTMANNOVÁ, Veronika and Lumír KREJČÍ

Basic information

Original name

In Vitro Characterization of Sumoylation of HR Proteins

Authors

ALTMANNOVÁ, Veronika (203 Czech Republic, guarantor, belonging to the institution) and Lumír KREJČÍ (203 Czech Republic, belonging to the institution)

Edition

TOTOWA, Homologous Recombination: Methods and Protocols, p. 483-502, 20 pp. Methods in Molecular Biology, volume 2153, 2021

Publisher

HUMANA PRESS INC

Other information

Language

English

Type of outcome

Kapitola resp. kapitoly v odborné knize

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Publication form

electronic version available online

References:

URL

RIV identification code

RIV/00216224:14110/21:00119654

Organization unit

Faculty of Medicine

ISBN

978-1-0716-0643-8

DOI

http://dx.doi.org/10.1007/978-1-0716-0644-5_33

UT WoS

000679479500034

Keywords in English

SUMO; Homologous recombination

Abstract

V originále

In vitro analysis of posttranslational modifications such as sumoylation provides a great tool to not only identify the target proteins but also to characterize the specific effects of this modification on the protein features and uncover possible regulatory mechanism. In this chapter, we will describe the purification of yeast SUMO machinery proteins and their use to identify SUMO modification of target proteins in vitro. Furthermore, we will show several examples characterizing the effect of sumoylation on the biochemical activities of various proteins involved in homologous recombination (HR) that helped to better understand the regulatory role of this modification.

Links

EF16_025/0007381, research and development project

Name: Preklinická progrese nových organických sloučenin s cílenou biologickou aktivitou

GA17-17720S, research and development project

Name: Vnitřní vlastnosti RAD51 vlákna a jeho biologické regulace

Investor: Czech Science Foundation

206292/E/17/Z, interní kód MU

Name: Mechanics and execution of homologous recombination - biophysics to the organism

Investor: Wellcome Trust

Citovat

ALTMANNOVÁ, Veronika and Lumír KREJČÍ. In Vitro Characterization of Sumoylation of HR Proteins. Online. In Andrés Aguilera; Aura Carreira. Homologous Recombination: Methods and Protocols. TOTOWA: HUMANA PRESS INC, 2021, p. 483-502. Methods in Molecular Biology, volume 2153. ISBN 978-1-0716-0643-8. Available from: https://dx.doi.org/10.1007/978-1-0716-0644-5_33.

@inbook{1834979,
   author = {Altmannová, Veronika and Krejčí, Lumír},
   address = {TOTOWA},
   booktitle = {Homologous Recombination: Methods and Protocols},
   doi = {http://dx.doi.org/10.1007/978-1-0716-0644-5_33},
   editor = {Andrés Aguilera; Aura Carreira},
   keywords = {SUMO; Homologous recombination},
   howpublished = {elektronická verze "online"},
   language = {eng},
   location = {TOTOWA},
   isbn = {978-1-0716-0643-8},
   note = {Korespondenční autor nereagoval na žádost o doplnění podílu. Podíl doplněn rovnocenně podle počtu participujících HS (dle rozhodnutí vedení PřF).},
   pages = {483-502},
   publisher = {HUMANA PRESS INC},
   title = {In Vitro Characterization of Sumoylation of HR Proteins},
   url = {https://link.springer.com/protocol/10.1007/978-1-0716-0644-5_33},
   year = {2021}
}

TY  - CHAP
ID  - 1834979
AU  - Altmannová, Veronika - Krejčí, Lumír
PY  - 2021
TI  - In Vitro Characterization of Sumoylation of HR Proteins
VL  - Methods in Molecular Biology, volume 2153
PB  - HUMANA PRESS INC
CY  - TOTOWA
SN  - 9781071606438
N1  - Korespondenční autor nereagoval na žádost o doplnění podílu. Podíl doplněn rovnocenně podle počtu participujících HS (dle rozhodnutí vedení PřF).
KW  - SUMO
KW  - Homologous recombination
UR  - https://link.springer.com/protocol/10.1007/978-1-0716-0644-5_33
N2  - In vitro analysis of posttranslational modifications such as sumoylation provides a great tool to not only identify the target proteins but also to characterize the specific effects of this modification on the protein features and uncover possible regulatory mechanism. In this chapter, we will describe the purification of yeast SUMO machinery proteins and their use to identify SUMO modification of target proteins in vitro. Furthermore, we will show several examples characterizing the effect of sumoylation on the biochemical activities of various proteins involved in homologous recombination (HR) that helped to better understand the regulatory role of this modification.
ER  -

ALTMANNOVÁ, Veronika and Lumír KREJČÍ. In Vitro Characterization of Sumoylation of HR Proteins. Online. In Andrés Aguilera; Aura Carreira. \textit{Homologous Recombination: Methods and Protocols}. TOTOWA: HUMANA PRESS INC, 2021, p.~483-502. Methods in Molecular Biology, volume 2153. ISBN~978-1-0716-0643-8. Available from: https://dx.doi.org/10.1007/978-1-0716-0644-5\_{}33.

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