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@inbook{1834979, author = {Altmannová, Veronika and Krejčí, Lumír}, address = {TOTOWA}, booktitle = {Homologous Recombination: Methods and Protocols}, doi = {http://dx.doi.org/10.1007/978-1-0716-0644-5_33}, editor = {Andrés Aguilera; Aura Carreira}, keywords = {SUMO; Homologous recombination}, howpublished = {elektronická verze "online"}, language = {eng}, location = {TOTOWA}, isbn = {978-1-0716-0643-8}, note = {Korespondenční autor nereagoval na žádost o doplnění podílu. Podíl doplněn rovnocenně podle počtu participujících HS (dle rozhodnutí vedení PřF).}, pages = {483-502}, publisher = {HUMANA PRESS INC}, title = {In Vitro Characterization of Sumoylation of HR Proteins}, url = {https://link.springer.com/protocol/10.1007/978-1-0716-0644-5_33}, year = {2021} }
TY - CHAP ID - 1834979 AU - Altmannová, Veronika - Krejčí, Lumír PY - 2021 TI - In Vitro Characterization of Sumoylation of HR Proteins VL - Methods in Molecular Biology, volume 2153 PB - HUMANA PRESS INC CY - TOTOWA SN - 9781071606438 N1 - Korespondenční autor nereagoval na žádost o doplnění podílu. Podíl doplněn rovnocenně podle počtu participujících HS (dle rozhodnutí vedení PřF). KW - SUMO KW - Homologous recombination UR - https://link.springer.com/protocol/10.1007/978-1-0716-0644-5_33 N2 - In vitro analysis of posttranslational modifications such as sumoylation provides a great tool to not only identify the target proteins but also to characterize the specific effects of this modification on the protein features and uncover possible regulatory mechanism. In this chapter, we will describe the purification of yeast SUMO machinery proteins and their use to identify SUMO modification of target proteins in vitro. Furthermore, we will show several examples characterizing the effect of sumoylation on the biochemical activities of various proteins involved in homologous recombination (HR) that helped to better understand the regulatory role of this modification. ER -
ALTMANNOVÁ, Veronika and Lumír KREJČÍ. In Vitro Characterization of Sumoylation of HR Proteins. Online. In Andrés Aguilera; Aura Carreira. \textit{Homologous Recombination: Methods and Protocols}. TOTOWA: HUMANA PRESS INC, 2021, p.~483-502. Methods in Molecular Biology, volume 2153. ISBN~978-1-0716-0643-8. Available from: https://dx.doi.org/10.1007/978-1-0716-0644-5\_{}33.
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