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@inbook{1834979,
author = {Altmannová, Veronika and Krejčí, Lumír},
address = {TOTOWA},
booktitle = {Homologous Recombination: Methods and Protocols},
doi = {http://dx.doi.org/10.1007/978-1-0716-0644-5_33},
editor = {Andrés Aguilera; Aura Carreira},
keywords = {SUMO; Homologous recombination},
howpublished = {elektronická verze "online"},
language = {eng},
location = {TOTOWA},
isbn = {978-1-0716-0643-8},
note = {Korespondenční autor nereagoval na žádost o doplnění podílu. Podíl doplněn rovnocenně podle počtu participujících HS (dle rozhodnutí vedení PřF).},
pages = {483-502},
publisher = {HUMANA PRESS INC},
title = {In Vitro Characterization of Sumoylation of HR Proteins},
url = {https://link.springer.com/protocol/10.1007/978-1-0716-0644-5_33},
year = {2021}
}
TY - CHAP
ID - 1834979
AU - Altmannová, Veronika - Krejčí, Lumír
PY - 2021
TI - In Vitro Characterization of Sumoylation of HR Proteins
VL - Methods in Molecular Biology, volume 2153
PB - HUMANA PRESS INC
CY - TOTOWA
SN - 9781071606438
N1 - Korespondenční autor nereagoval na žádost o doplnění podílu. Podíl doplněn rovnocenně podle počtu participujících HS (dle rozhodnutí vedení PřF).
KW - SUMO
KW - Homologous recombination
UR - https://link.springer.com/protocol/10.1007/978-1-0716-0644-5_33
N2 - In vitro analysis of posttranslational modifications such as sumoylation provides a great tool to not only identify the target proteins but also to characterize the specific effects of this modification on the protein features and uncover possible regulatory mechanism. In this chapter, we will describe the purification of yeast SUMO machinery proteins and their use to identify SUMO modification of target proteins in vitro. Furthermore, we will show several examples characterizing the effect of sumoylation on the biochemical activities of various proteins involved in homologous recombination (HR) that helped to better understand the regulatory role of this modification.
ER -
ALTMANNOVÁ, Veronika and Lumír KREJČÍ. In Vitro Characterization of Sumoylation of HR Proteins. Online. In Andrés Aguilera; Aura Carreira. \textit{Homologous Recombination: Methods and Protocols}. TOTOWA: HUMANA PRESS INC, 2021, p.~483-502. Methods in Molecular Biology, volume 2153. ISBN~978-1-0716-0643-8. Available from: https://dx.doi.org/10.1007/978-1-0716-0644-5\_{}33.
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