1H NMR Profiling of Honey Bee Bodies Revealed Metabolic
Differences between Summer and Winter Bees

J 2022

1H NMR Profiling of Honey Bee Bodies Revealed Metabolic Differences between Summer and Winter Bees

LEE, Saetbyeol, Filip KALCIC, Iola F. DUARTE, Dalibor TITERA, Martin KAMLER et. al.

Basic information

Original name

1H NMR Profiling of Honey Bee Bodies Revealed Metabolic Differences between Summer and Winter Bees

Authors

LEE, Saetbyeol, Filip KALCIC, Iola F. DUARTE, Dalibor TITERA, Martin KAMLER, Pavel MRNA, Pavel HYRŠL (203 Czech Republic, belonging to the institution), Jiri DANIHLIK, Pavel DOBEŠ (203 Czech Republic, belonging to the institution), Martin KUNC (203 Czech Republic, belonging to the institution), Anna PUDLO and Jaroslav HAVLIK (guarantor)

Edition

Insects, MDPI, 2022, 2075-4450

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10616 Entomology

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.000

RIV identification code

RIV/00216224:14310/22:00119768

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.3390/insects13020193

UT WoS

000769716500001

Keywords in English

Apis mellifera; winter bees; nuclear magnetic resonance; metabolome; longevity

Abstract

V originále

In temperate climates, honey bee workers of the species Apis mellifera have different lifespans depending on the seasonal phenotype: summer bees (short lifespan) and winter bees (long lifespan). Many studies have revealed the biochemical parameters involved in the lifespan differentiation of summer and winter bees. However, comprehensive information regarding the metabolic changes occurring in their bodies between the two is limited. This study used proton nuclear magnetic resonance (1H NMR) spectroscopy to analyze the metabolic differences between summer and winter bees of the same age. The multivariate analysis showed that summer and winter bees could be distinguished based on their metabolic profiles. Among the 36 metabolites found, 28 metabolites have displayed significant changes from summer to winter bees. Compared to summer bees, trehalose in winter bees showed 1.9 times higher concentration, and all amino acids except for proline and alanine showed decreased patterns. We have also detected an unknown compound, with a CH3 singlet at 2.83 ppm, which is a potential biomarker that is about 13 times higher in summer bees. Our results show that the metabolites in summer and winter bees have distinctive characteristics; this information could provide new insights and support further studies on honey bee longevity and overwintering.

Links

QJ1610248, research and development project

Name: Dlouhověkost včel a její úloha v udržitelném chovu (Acronym: Dlouhověkost)

Investor: Ministry of Agriculture of the CR

QK1910286, research and development project

Name: Efektivní postupy a strategie pro zvládání včelích chorob a udržitelný chov včelstev

Investor: Ministry of Agriculture of the CR

Citovat

LEE, Saetbyeol, Filip KALCIC, Iola F. DUARTE, Dalibor TITERA, Martin KAMLER, Pavel MRNA, Pavel HYRŠL, Jiri DANIHLIK, Pavel DOBEŠ, Martin KUNC, Anna PUDLO and Jaroslav HAVLIK. 1H NMR Profiling of Honey Bee Bodies Revealed Metabolic Differences between Summer and Winter Bees. Insects. MDPI, 2022, vol. 13, No 2, p. 1-13. ISSN 2075-4450. Available from: https://dx.doi.org/10.3390/insects13020193.

@article{1835003,
   author = {Lee, Saetbyeol and Kalcic, Filip and Duarte, Iola F. and Titera, Dalibor and Kamler, Martin and Mrna, Pavel and Hyršl, Pavel and Danihlik, Jiri and Dobeš, Pavel and Kunc, Martin and Pudlo, Anna and Havlik, Jaroslav},
   article_number = {2},
   doi = {http://dx.doi.org/10.3390/insects13020193},
   keywords = {Apis mellifera; winter bees; nuclear magnetic resonance; metabolome; longevity},
   language = {eng},
   issn = {2075-4450},
   journal = {Insects},
   title = {1H NMR Profiling of Honey Bee Bodies Revealed Metabolic Differences between Summer and Winter Bees},
   url = {https://www.mdpi.com/2075-4450/13/2/193},
   volume = {13},
   year = {2022}
}

TY  - JOUR
ID  - 1835003
AU  - Lee, Saetbyeol - Kalcic, Filip - Duarte, Iola F. - Titera, Dalibor - Kamler, Martin - Mrna, Pavel - Hyršl, Pavel - Danihlik, Jiri - Dobeš, Pavel - Kunc, Martin - Pudlo, Anna - Havlik, Jaroslav
PY  - 2022
TI  - 1H NMR Profiling of Honey Bee Bodies Revealed Metabolic Differences between Summer and Winter Bees
JF  - Insects
VL  - 13
IS  - 2
SP  - 1-13
EP  - 1-13
PB  - MDPI
SN  - 20754450
KW  - Apis mellifera
KW  - winter bees
KW  - nuclear magnetic resonance
KW  - metabolome
KW  - longevity
UR  - https://www.mdpi.com/2075-4450/13/2/193
N2  - In temperate climates, honey bee workers of the species Apis mellifera have different lifespans depending on the seasonal phenotype: summer bees (short lifespan) and winter bees (long lifespan). Many studies have revealed the biochemical parameters involved in the lifespan differentiation of summer and winter bees. However, comprehensive information regarding the metabolic changes occurring in their bodies between the two is limited. This study used proton nuclear magnetic resonance (1H NMR) spectroscopy to analyze the metabolic differences between summer and winter bees of the same age. The multivariate analysis showed that summer and winter bees could be distinguished based on their metabolic profiles. Among the 36 metabolites found, 28 metabolites have displayed significant changes from summer to winter bees. Compared to summer bees, trehalose in winter bees showed 1.9 times higher concentration, and all amino acids except for proline and alanine showed decreased patterns. We have also detected an unknown compound, with a CH3 singlet at 2.83 ppm, which is a potential biomarker that is about 13 times higher in summer bees. Our results show that the metabolites in summer and winter bees have distinctive characteristics; this information could provide new insights and support further studies on honey bee longevity and overwintering.
ER  -

LEE, Saetbyeol, Filip KALCIC, Iola F. DUARTE, Dalibor TITERA, Martin KAMLER, Pavel MRNA, Pavel HYRŠL, Jiri DANIHLIK, Pavel DOBEŠ, Martin KUNC, Anna PUDLO and Jaroslav HAVLIK. 1H NMR Profiling of Honey Bee Bodies Revealed Metabolic Differences between Summer and Winter Bees. \textit{Insects}. MDPI, 2022, vol.~13, No~2, p.~1-13. ISSN~2075-4450. Available from: https://dx.doi.org/10.3390/insects13020193.

Detailed Information on Publication Record