| TREML, Jakub, P. VEČEŘOVÁ, Petra HERCZOGOVÁ and Karel ŠMEJKAL. Direct and Indirect Antioxidant Effects of Selected Plant Phenolics in Cell-Based Assays. Molecules. Basel: MDPI, 2021, vol. 26, No 9, p. 1-15. ISSN 1420-3049. Available from: https://dx.doi.org/10.3390/molecules26092534. |
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@article{1835161,
author = {Treml, Jakub and Večeřová, P. and Herczogová, Petra and Šmejkal, Karel},
article_location = {Basel},
article_number = {9},
doi = {http://dx.doi.org/10.3390/molecules26092534},
keywords = {antioxidants; CAA; catalase; glucose toxicity; plant phenolics; superoxide dismutase; Nrf2-ARE},
language = {eng},
issn = {1420-3049},
journal = {Molecules},
title = {Direct and Indirect Antioxidant Effects of Selected Plant Phenolics in Cell-Based Assays},
url = {https://www.mdpi.com/1420-3049/26/9/2534/htm#},
volume = {26},
year = {2021}
}
TY - JOUR
ID - 1835161
AU - Treml, Jakub - Večeřová, P. - Herczogová, Petra - Šmejkal, Karel
PY - 2021
TI - Direct and Indirect Antioxidant Effects of Selected Plant Phenolics in Cell-Based Assays
JF - Molecules
VL - 26
IS - 9
SP - 1-15
EP - 1-15
PB - MDPI
SN - 14203049
KW - antioxidants
KW - CAA
KW - catalase
KW - glucose toxicity
KW - plant phenolics
KW - superoxide dismutase
KW - Nrf2-ARE
UR - https://www.mdpi.com/1420-3049/26/9/2534/htm#
N2 - Background: Oxidative stress is a key factor in the pathophysiology of many diseases. This study aimed to verify the antioxidant activity of selected plant phenolics in cell-based assays and determine their direct or indirect effects. Methods: The cellular antioxidant assay (CAA) assay was employed for direct scavenging assays. In the indirect approach, the influence of each test substance on the gene and protein expression and activity of selected antioxidant enzymes was observed. One assay also dealt with activation of the Nrf2-ARE pathway. The overall effect of each compound was measured using a glucose oxidative stress protection assay. Results: Among the test compounds, acteoside showed the highest direct scavenging activity and no effect on the expression of antioxidant enzymes. It increased only the activity of catalase. Diplacone was less active in direct antioxidant assays but positively affected enzyme expression and catalase activity. Morusin showed no antioxidant activity in the CAA assay. Similarly, pomiferin had only mild antioxidant activity and proved rather cytotoxic. Conclusions: Of the four selected phenolics, only acteoside and diplacone demonstrated antioxidant effects in cell-based assays.
ER -
TREML, Jakub, P. VEČEŘOVÁ, Petra HERCZOGOVÁ and Karel ŠMEJKAL. Direct and Indirect Antioxidant Effects of Selected Plant Phenolics in Cell-Based Assays. \textit{Molecules}. Basel: MDPI, 2021, vol.~26, No~9, p.~1-15. ISSN~1420-3049. Available from: https://dx.doi.org/10.3390/molecules26092534.
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