J 2022

Paraphocaeicola brunensis gen. nov., sp. nov., Carrying Two Variants of nimB Resistance Gene from Bacteroides fragilis, and Caecibacteroides pullorum gen. nov., sp. nov., Two Novel Genera Isolated from Chicken Caeca

KRÁLOVÁ, Stanislava, Lenka DAVIDOVA-GERZOVA, Adam VALCEK, Matěj BEZDÍČEK, Ivan RYCHLÍK et. al.

Basic information

Original name

Paraphocaeicola brunensis gen. nov., sp. nov., Carrying Two Variants of nimB Resistance Gene from Bacteroides fragilis, and Caecibacteroides pullorum gen. nov., sp. nov., Two Novel Genera Isolated from Chicken Caeca

Authors

KRÁLOVÁ, Stanislava (703 Slovakia, guarantor, belonging to the institution), Lenka DAVIDOVA-GERZOVA, Adam VALCEK, Matěj BEZDÍČEK (203 Czech Republic, belonging to the institution), Ivan RYCHLÍK (203 Czech Republic), Veronika REZACOVA and Alois ČÍŽEK (203 Czech Republic)

Edition

Microbiology Spectrum, American Society for Microbiology, 2022, 2165-0497

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10606 Microbiology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 3.700

RIV identification code

RIV/00216224:14310/22:00125419

Organization unit

Faculty of Science

DOI

UT WoS

000766015800155

Keywords in English

phylogenomics; polyphasic taxonomy; Paraphocaeicola brunensis gen. nov.; sp. nov.; Caecibacteroides pullorum gen. nov.; sp. nov.; Bacteroidaceae; metronidazole resistance; nimB gene

Tags

Tags

International impact, Reviewed
Změněno: 9/12/2022 13:04, Mgr. Marie Šípková, DiS.

Abstract

V originále

Three difficult-to-cultivate, strictly anaerobic strains, AN20T, AN421T, and AN502, were analyzed within a project studying possible probiotics for newly hatched chickens. Phylogenetic analyses showed that strains AN20T, AN421T, and AN502 formed two well-separated phylogenetic lineages in all phylogenetic and phylogenomic trees comprising members of the family Bacteroidaceae. Comparison to reference genomes of type species Bacteroides fragilis NCTC 9343T, Phocaeicola abscessus CCUG 55929T, and Capsularis zoogleoformans ATCC 33285T showed low relatedness based on the calculated genome-to-genome distance and orthologous average nucleotide identity. Analysis of fatty acid profiles showed iso-C15:0, anteiso-C15:0, C16:0, C18:1 ω9c, and iso-C17:0 3OH as the major fatty acids for all three strains and additionally C16:0 3OH for AN421T and AN502. A specific combination of respiratory quinones different from related taxa was found in analyzed strains, MK-5 plus MK-11 in strain AN20T and MK-5 plus MK-10 in strains AN421T and AN502. Strains AN421T and AN502 harbor complete CRISPR loci with CRISPR array, type II-C, accompanied by a set of cas genes (cas9, cas1, and cas2) in close proximity. Interestingly, strain AN20T was found to harbor two copies of nimB gene with >95% similarity to nimB of B. fragilis, suggesting a horizontal gene transfer between these taxa. In summary, three isolates characterized in this study represent two novel species, which we proposed to be classified in two novel genera of the family Bacteroidaceae, for which the names Paraphocaeicola brunensis sp. nov. (AN20T = CCM 9041T = DSM 111154T) and Caecibacteroides pullorum sp. nov. (AN421T= CCM 9040T = DSM 111155T) are proposed.