KRÁLOVÁ, Stanislava, Lenka DAVIDOVA-GERZOVA, Adam VALCEK, Matěj BEZDÍČEK, Ivan RYCHLÍK, Veronika REZACOVA and Alois ČÍŽEK. Paraphocaeicola brunensis gen. nov., sp. nov., Carrying Two Variants of nimB Resistance Gene from Bacteroides fragilis, and Caecibacteroides pullorum gen. nov., sp. nov., Two Novel Genera Isolated from Chicken Caeca. Microbiology Spectrum. American Society for Microbiology, 2022, vol. 10, No 1, p. 1-21. ISSN 2165-0497. Available from: https://dx.doi.org/10.1128/spectrum.01954-21.
Other formats:   BibTeX LaTeX RIS
Basic information
Original name Paraphocaeicola brunensis gen. nov., sp. nov., Carrying Two Variants of nimB Resistance Gene from Bacteroides fragilis, and Caecibacteroides pullorum gen. nov., sp. nov., Two Novel Genera Isolated from Chicken Caeca
Authors KRÁLOVÁ, Stanislava (703 Slovakia, guarantor, belonging to the institution), Lenka DAVIDOVA-GERZOVA, Adam VALCEK, Matěj BEZDÍČEK (203 Czech Republic, belonging to the institution), Ivan RYCHLÍK (203 Czech Republic), Veronika REZACOVA and Alois ČÍŽEK (203 Czech Republic).
Edition Microbiology Spectrum, American Society for Microbiology, 2022, 2165-0497.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10606 Microbiology
Country of publisher United States of America
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 3.700
RIV identification code RIV/00216224:14310/22:00125419
Organization unit Faculty of Science
Doi http://dx.doi.org/10.1128/spectrum.01954-21
UT WoS 000766015800155
Keywords in English phylogenomics; polyphasic taxonomy; Paraphocaeicola brunensis gen. nov.; sp. nov.; Caecibacteroides pullorum gen. nov.; sp. nov.; Bacteroidaceae; metronidazole resistance; nimB gene
Tags 14110212, podil, rivok
Tags International impact, Reviewed
Changed by Changed by: Mgr. Marie Šípková, DiS., učo 437722. Changed: 9/12/2022 13:04.
Abstract
Three difficult-to-cultivate, strictly anaerobic strains, AN20T, AN421T, and AN502, were analyzed within a project studying possible probiotics for newly hatched chickens. Phylogenetic analyses showed that strains AN20T, AN421T, and AN502 formed two well-separated phylogenetic lineages in all phylogenetic and phylogenomic trees comprising members of the family Bacteroidaceae. Comparison to reference genomes of type species Bacteroides fragilis NCTC 9343T, Phocaeicola abscessus CCUG 55929T, and Capsularis zoogleoformans ATCC 33285T showed low relatedness based on the calculated genome-to-genome distance and orthologous average nucleotide identity. Analysis of fatty acid profiles showed iso-C15:0, anteiso-C15:0, C16:0, C18:1 ω9c, and iso-C17:0 3OH as the major fatty acids for all three strains and additionally C16:0 3OH for AN421T and AN502. A specific combination of respiratory quinones different from related taxa was found in analyzed strains, MK-5 plus MK-11 in strain AN20T and MK-5 plus MK-10 in strains AN421T and AN502. Strains AN421T and AN502 harbor complete CRISPR loci with CRISPR array, type II-C, accompanied by a set of cas genes (cas9, cas1, and cas2) in close proximity. Interestingly, strain AN20T was found to harbor two copies of nimB gene with >95% similarity to nimB of B. fragilis, suggesting a horizontal gene transfer between these taxa. In summary, three isolates characterized in this study represent two novel species, which we proposed to be classified in two novel genera of the family Bacteroidaceae, for which the names Paraphocaeicola brunensis sp. nov. (AN20T = CCM 9041T = DSM 111154T) and Caecibacteroides pullorum sp. nov. (AN421T= CCM 9040T = DSM 111155T) are proposed.
PrintDisplayed: 22/7/2024 16:26