Objectives: Overweight and obesity are risk factors for many diseases, nutrition leading to these phenomena is not only a question of disbalance between energy intake and expenditure, but also the presence of micronutrients. In our study, we focused on measuring residues of chromium, zinc and iron in the hair of men with different BMI. Methods: Hair samples and anthropometric questionnaires were collected from 45 males. Numbers of subjects and age structure were comparable between the three BMI groups. The determination of metal levels was performed by inductively coupled plasma mass spectrometry after mineralization of the hair. Results: The hair of obese men contained significantly higher chromium (0.096 mu g/g vs. 0.045 mu g/g, p =0.0039) and iron (9.42 mu g/g vs. 5.84 mu g/g, p =0.0009) concentrations than that of overweight men, but no significant difference between the normal-weight group and the obese group were found. The concentration of zinc was lower in obese subjects compared to overweight subjects (183.5 mu g/g vs. 206.2 mu g/g, p = 0.038). Also, statistically significant correlations between chromium and iron concentrations in hair and BMI were found (r =0.307, p =0.040, r = 0.360, p =0.015, respectively). According to our results, age did not significantly affect chromium, iron and zinc concentrations in hair. Conclusion: Consistent with some published studies, we have found that obese men have higher chromium and iron concentrations and lower zinc concentrations in hair.