J 2021

Sheep as a Potential Model of Intradiscal Infection by the Bacterium Cutibacterium acnes

COSCIA, E.C., N.S. ABUTALEB, B. HOSTETTER, M.N. SELEEM, G.J. BREUR et. al.

Basic information

Original name

Sheep as a Potential Model of Intradiscal Infection by the Bacterium Cutibacterium acnes

Authors

COSCIA, E.C., N.S. ABUTALEB, B. HOSTETTER, M.N. SELEEM, G.J. BREUR, R.R. MCCAIN, C.J. CRAIN, Ondřej SLABÝ (203 Czech Republic, guarantor, belonging to the institution), M.N. CAPOOR, A. MCDOWELL, externista FAHAD AHMED (840 United States of America), V. VIJAYANPILLAI, S.K. NARAYANAN and M.F. COSCIA

Edition

Polish Journal of Veterinary Sciences, Olsztyn, Polish Academy of Sciences Committee of Veterinary Sciences, University of Warmia and Mazury in Olsztyn, 2021, 2306-7381

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

40301 Veterinary science

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 2.518

RIV identification code

RIV/00216224:14740/21:00124251

Organization unit

Central European Institute of Technology

DOI

UT WoS

000634239500001

Keywords in English

Cutibacterium acnes; sheep model; spinal intervertebral discs; percutaneous injections; bacterial discitis

Tags

Tags

International impact, Reviewed
Změněno: 18/5/2022 13:39, Mgr. Pavla Foltynová, Ph.D.

Abstract

V originále

The anaerobic bacterium Cutibacterium acnes has been increasingly linked to the development of degenerative disc disease (DDD), although causality is yet to be conclusively proven. To better study how this organism could contribute to the aetiology of DDD, improved animal models that are more reflective of human disc anatomy, biology and mechanical properties are required. Against this background, our proof-of concept study aimed to be the first demonstration that C. acnes could be safely administered percutaneously into sheep intervertebral discs (IVDs) for in vivo study. Following our protocol, two sheep were successfully injected with a strain of C. acnes (8.3 x 10(6) CFU/disc) previously recovered from a human degenerative disc. No adverse reactions were noted, and at one-month post inoculation all triplicate infected discs in our first animal grew C. acnes, albeit at a reduced load (5.12 x 10(4) to 6.67 x 10(4) CFU/disc). At six months, no growth was detected in discs from our second animal indicating bacterial clearance. This pilot study has demonstrated the feasibility of safe percutaneous injection of C. acnes into sheep IVDs under fluoroscopic guidance. The design of follow-up sheep studies to investigate the potential of C. acnes to drive pathological changes within infected discs should now be pursued.