Complex Alterations of Fatty Acid Metabolism and
Phospholipidome Uncovered in Isolated Colon Cancer Epithelial
Cells

J 2021

Complex Alterations of Fatty Acid Metabolism and Phospholipidome Uncovered in Isolated Colon Cancer Epithelial Cells

HOFMANOVÁ, Jiřina, Josef SLAVIK, Miroslav CIGANEK, Petra OVESNÁ, Zuzana TYLICHOVA et. al.

Basic information

Original name

Complex Alterations of Fatty Acid Metabolism and Phospholipidome Uncovered in Isolated Colon Cancer Epithelial Cells

Authors

HOFMANOVÁ, Jiřina (203 Czech Republic, belonging to the institution), Josef SLAVIK (203 Czech Republic), Miroslav CIGANEK (203 Czech Republic), Petra OVESNÁ (203 Czech Republic, belonging to the institution), Zuzana TYLICHOVA (203 Czech Republic), Martina KARASOVÁ (203 Czech Republic, belonging to the institution), Ondrej ZAPLETAL (203 Czech Republic), Nicol STRAKOVA (203 Czech Republic), Jirina PROCHAZKOVA (203 Czech Republic), Jan BOUCHAL (203 Czech Republic), Zdenek KOLAR (203 Czech Republic), Jiri EHRMANN (203 Czech Republic), Monika LEVKOVA (203 Czech Republic), Zlatka HUSKOVA (203 Czech Republic), Pavel SKALICKY (203 Czech Republic), Alois KOZUBÍK (203 Czech Republic, belonging to the institution), Miroslav MACHALA (203 Czech Republic) and Jan VONDRÁČEK (203 Czech Republic, guarantor)

Edition

International Journal of Molecular Sciences, Basel, Multidisciplinary Digital Publishing Institute, 2021, 1422-0067

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 6.208

RIV identification code

RIV/00216224:14310/21:00124278

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.3390/ijms22136650

UT WoS

000671202800001

Keywords in English

phospholipids; lysophospholipids; fatty acid synthesis; desaturation; colorectal carcinoma; EpCAM; epithelial cells; lipidomics

Abstract

V originále

The development of colon cancer, one of the most common malignancies, is accompanied with numerous lipid alterations. However, analyses of whole tumor samples may not always provide an accurate description of specific changes occurring directly in tumor epithelial cells. Here, we analyzed in detail the phospholipid (PL), lysophospholipid (lysoPL), and fatty acid (FA) profiles of purified EpCAM(+) cells, isolated from tumor and adjacent non-tumor tissues of colon cancer patients. We found that a number of FAs increased significantly in isolated tumor cells, which also included a number of long polyunsaturated FAs. Higher levels of FAs were associated with increased expression of FA synthesis genes, as well as with altered expression of enzymes involved in FA elongation and desaturation, including particularly fatty acid synthase, stearoyl-CoA desaturase, fatty acid desaturase 2 and ELOVL5 fatty acid elongase 5 We identified significant changes in ratios of specific lysoPLs and corresponding PLs. A number of lysophosphatidylcholine and lysophosphatidylethanolamine species, containing long-chain and very-long chain FAs, often with high numbers of double bonds, were significantly upregulated in tumor cells. Increased de novo synthesis of very long-chain FAs, or, altered uptake or incorporation of these FAs into specific lysoPLs in tumor cells, may thus contribute to reprogramming of cellular phospholipidome and membrane alterations observed in colon cancer.

Citovat

HOFMANOVÁ, Jiřina, Josef SLAVIK, Miroslav CIGANEK, Petra OVESNÁ, Zuzana TYLICHOVA, Martina KARASOVÁ, Ondrej ZAPLETAL, Nicol STRAKOVA, Jirina PROCHAZKOVA, Jan BOUCHAL, Zdenek KOLAR, Jiri EHRMANN, Monika LEVKOVA, Zlatka HUSKOVA, Pavel SKALICKY, Alois KOZUBÍK, Miroslav MACHALA and Jan VONDRÁČEK. Complex Alterations of Fatty Acid Metabolism and Phospholipidome Uncovered in Isolated Colon Cancer Epithelial Cells. International Journal of Molecular Sciences. Basel: Multidisciplinary Digital Publishing Institute, 2021, vol. 22, No 13, p. 1-16. ISSN 1422-0067. Available from: https://dx.doi.org/10.3390/ijms22136650.

@article{1836814,
   author = {Hofmanová, Jiřina and Slavik, Josef and Ciganek, Miroslav and Ovesná, Petra and Tylichova, Zuzana and Karasová, Martina and Zapletal, Ondrej and Strakova, Nicol and Prochazkova, Jirina and Bouchal, Jan and Kolar, Zdenek and Ehrmann, Jiri and Levkova, Monika and Huskova, Zlatka and Skalicky, Pavel and Kozubík, Alois and Machala, Miroslav and Vondráček, Jan},
   article_location = {Basel},
   article_number = {13},
   doi = {http://dx.doi.org/10.3390/ijms22136650},
   keywords = {phospholipids; lysophospholipids; fatty acid synthesis; desaturation; colorectal carcinoma; EpCAM; epithelial cells; lipidomics},
   language = {eng},
   issn = {1422-0067},
   journal = {International Journal of Molecular Sciences},
   title = {Complex Alterations of Fatty Acid Metabolism and Phospholipidome Uncovered in Isolated Colon Cancer Epithelial Cells},
   url = {https://www.mdpi.com/1422-0067/22/13/6650},
   volume = {22},
   year = {2021}
}

TY  - JOUR
ID  - 1836814
AU  - Hofmanová, Jiřina - Slavik, Josef - Ciganek, Miroslav - Ovesná, Petra - Tylichova, Zuzana - Karasová, Martina - Zapletal, Ondrej - Strakova, Nicol - Prochazkova, Jirina - Bouchal, Jan - Kolar, Zdenek - Ehrmann, Jiri - Levkova, Monika - Huskova, Zlatka - Skalicky, Pavel - Kozubík, Alois - Machala, Miroslav - Vondráček, Jan
PY  - 2021
TI  - Complex Alterations of Fatty Acid Metabolism and Phospholipidome Uncovered in Isolated Colon Cancer Epithelial Cells
JF  - International Journal of Molecular Sciences
VL  - 22
IS  - 13
SP  - 1-16
EP  - 1-16
PB  - Multidisciplinary Digital Publishing Institute
SN  - 14220067
KW  - phospholipids
KW  - lysophospholipids
KW  - fatty acid synthesis
KW  - desaturation
KW  - colorectal carcinoma
KW  - EpCAM
KW  - epithelial cells
KW  - lipidomics
UR  - https://www.mdpi.com/1422-0067/22/13/6650
N2  - The development of colon cancer, one of the most common malignancies, is accompanied with numerous lipid alterations. However, analyses of whole tumor samples may not always provide an accurate description of specific changes occurring directly in tumor epithelial cells. Here, we analyzed in detail the phospholipid (PL), lysophospholipid (lysoPL), and fatty acid (FA) profiles of purified EpCAM(+) cells, isolated from tumor and adjacent non-tumor tissues of colon cancer patients. We found that a number of FAs increased significantly in isolated tumor cells, which also included a number of long polyunsaturated FAs. Higher levels of FAs were associated with increased expression of FA synthesis genes, as well as with altered expression of enzymes involved in FA elongation and desaturation, including particularly fatty acid synthase, stearoyl-CoA desaturase, fatty acid desaturase 2 and ELOVL5 fatty acid elongase 5 We identified significant changes in ratios of specific lysoPLs and corresponding PLs. A number of lysophosphatidylcholine and lysophosphatidylethanolamine species, containing long-chain and very-long chain FAs, often with high numbers of double bonds, were significantly upregulated in tumor cells. Increased de novo synthesis of very long-chain FAs, or, altered uptake or incorporation of these FAs into specific lysoPLs in tumor cells, may thus contribute to reprogramming of cellular phospholipidome and membrane alterations observed in colon cancer.
ER  -

HOFMANOVÁ, Jiřina, Josef SLAVIK, Miroslav CIGANEK, Petra OVESNÁ, Zuzana TYLICHOVA, Martina KARASOVÁ, Ondrej ZAPLETAL, Nicol STRAKOVA, Jirina PROCHAZKOVA, Jan BOUCHAL, Zdenek KOLAR, Jiri EHRMANN, Monika LEVKOVA, Zlatka HUSKOVA, Pavel SKALICKY, Alois KOZUBÍK, Miroslav MACHALA and Jan VONDRÁČEK. Complex Alterations of Fatty Acid Metabolism and Phospholipidome Uncovered in Isolated Colon Cancer Epithelial Cells. \textit{International Journal of Molecular Sciences}. Basel: Multidisciplinary Digital Publishing Institute, 2021, vol.~22, No~13, p.~1-16. ISSN~1422-0067. Available from: https://dx.doi.org/10.3390/ijms22136650.

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