Complete Genome Sequence of the Type Strain Tepidimonas
taiwanensis LMG 22826T, a Thermophilic Alkaline Protease and
Polyhydroxyalkanoate Producer

J 2021

Complete Genome Sequence of the Type Strain Tepidimonas taiwanensis LMG 22826T, a Thermophilic Alkaline Protease and Polyhydroxyalkanoate Producer

HERMANKOVA, Kristyna, Xenie KOURILOVA, Iva PERNICOVA, Matěj BEZDÍČEK, Martina LENGEROVÁ et. al.

Basic information

Original name

Complete Genome Sequence of the Type Strain Tepidimonas taiwanensis LMG 22826T, a Thermophilic Alkaline Protease and Polyhydroxyalkanoate Producer

Authors

HERMANKOVA, Kristyna (203 Czech Republic), Xenie KOURILOVA (203 Czech Republic), Iva PERNICOVA (203 Czech Republic), Matěj BEZDÍČEK (203 Czech Republic, belonging to the institution), Martina LENGEROVÁ (203 Czech Republic, belonging to the institution), Stanislav OBRUCA (203 Czech Republic) and Karel SEDLAR (203 Czech Republic, guarantor)

Edition

GENOME BIOLOGY AND EVOLUTION, OXFORD, OXFORD UNIV PRESS, 2021, 1759-6653

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30101 Human genetics

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.065

RIV identification code

RIV/00216224:14110/21:00124283

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1093/gbe/evab280

UT WoS

000809682900019

Keywords in English

Tepidimonas taiwanensis; polyhydroxyalkanoates; hybrid assembly; Oxford Nanopore Technologies; functional annotation; alkaline protease

Abstract

V originále

Tepidimonas taiwanensis is a moderately thermophilic, Gram-negative, rod-shaped, chemoorganoheterotrophic, motile bacterium. The alkaline protease producing type strain T. taiwanensis LMG 22826T was recently reported to also be a promising producer of polyhydroxyalkanoates (PHAs)—renewable and biodegradable polymers representing an alternative to conventional plastics. Here, we present its first complete genome sequence which is also the first complete genome sequence of the whole species. The genome consists of a single 2,915,587-bp-long circular chromosome with GC content of 68.75%. Genome annotation identified 2,764 genes in total while 2,634 open reading frames belonged to protein-coding genes. Although functional annotation of the genome and division of genes into Clusters of Orthologous Groups (COGs) revealed a relatively high number of 694 genes with unknown function or unknown COG, the majority of genes were assigned a function. Most of the genes, 406 in total, were involved in energy production and conversion, and amino acid transport and metabolism. Moreover, particular key genes involved in the metabolism of PHA were identified. Knowledge of the genome in connection with the recently reported ability to produce bioplastics from the waste stream of wine production makes T. taiwanensis LMG 22826T, an ideal candidate for further genome engineering as a bacterium with high biotechnological potential.

Citovat

HERMANKOVA, Kristyna, Xenie KOURILOVA, Iva PERNICOVA, Matěj BEZDÍČEK, Martina LENGEROVÁ, Stanislav OBRUCA and Karel SEDLAR. Complete Genome Sequence of the Type Strain Tepidimonas taiwanensis LMG 22826T, a Thermophilic Alkaline Protease and Polyhydroxyalkanoate Producer. GENOME BIOLOGY AND EVOLUTION. OXFORD: OXFORD UNIV PRESS, 2021, vol. 13, No 12, p. 1-5. ISSN 1759-6653. Available from: https://dx.doi.org/10.1093/gbe/evab280.

@article{1837019,
   author = {Hermankova, Kristyna and Kourilova, Xenie and Pernicova, Iva and Bezdíček, Matěj and Lengerová, Martina and Obruca, Stanislav and Sedlar, Karel},
   article_location = {OXFORD},
   article_number = {12},
   doi = {http://dx.doi.org/10.1093/gbe/evab280},
   keywords = {Tepidimonas taiwanensis; polyhydroxyalkanoates; hybrid assembly; Oxford Nanopore Technologies; functional annotation; alkaline protease},
   language = {eng},
   issn = {1759-6653},
   journal = {GENOME BIOLOGY AND EVOLUTION},
   note = {Bez afiliace k MU.},
   title = {Complete Genome Sequence of the Type Strain Tepidimonas taiwanensis LMG 22826T, a Thermophilic Alkaline Protease and Polyhydroxyalkanoate Producer},
   url = {https://academic.oup.com/gbe/article/13/12/evab280/6462190},
   volume = {13},
   year = {2021}
}

TY  - JOUR
ID  - 1837019
AU  - Hermankova, Kristyna - Kourilova, Xenie - Pernicova, Iva - Bezdíček, Matěj - Lengerová, Martina - Obruca, Stanislav - Sedlar, Karel
PY  - 2021
TI  - Complete Genome Sequence of the Type Strain Tepidimonas taiwanensis LMG 22826T, a Thermophilic Alkaline Protease and Polyhydroxyalkanoate Producer
JF  - GENOME BIOLOGY AND EVOLUTION
VL  - 13
IS  - 12
SP  - 1-5
EP  - 1-5
PB  - OXFORD UNIV PRESS
SN  - 17596653
N1  - Bez afiliace k MU.
KW  - Tepidimonas taiwanensis
KW  - polyhydroxyalkanoates
KW  - hybrid assembly
KW  - Oxford Nanopore Technologies
KW  - functional annotation
KW  - alkaline protease
UR  - https://academic.oup.com/gbe/article/13/12/evab280/6462190
N2  - Tepidimonas taiwanensis is a moderately thermophilic, Gram-negative, rod-shaped, chemoorganoheterotrophic, motile bacterium. The alkaline protease producing type strain T. taiwanensis LMG 22826T was recently reported to also be a promising producer of polyhydroxyalkanoates (PHAs)—renewable and biodegradable polymers representing an alternative to conventional plastics. Here, we present its first complete genome sequence which is also the first complete genome sequence of the whole species. The genome consists of a single 2,915,587-bp-long circular chromosome with GC content of 68.75%. Genome annotation identified 2,764 genes in total while 2,634 open reading frames belonged to protein-coding genes. Although functional annotation of the genome and division of genes into Clusters of Orthologous Groups (COGs) revealed a relatively high number of 694 genes with unknown function or unknown COG, the majority of genes were assigned a function. Most of the genes, 406 in total, were involved in energy production and conversion, and amino acid transport and metabolism. Moreover, particular key genes involved in the metabolism of PHA were identified. Knowledge of the genome in connection with the recently reported ability to produce bioplastics from the waste stream of wine production makes T. taiwanensis LMG 22826T, an ideal candidate for further genome engineering as a bacterium with high biotechnological potential.
ER  -

HERMANKOVA, Kristyna, Xenie KOURILOVA, Iva PERNICOVA, Matěj BEZDÍČEK, Martina LENGEROVÁ, Stanislav OBRUCA and Karel SEDLAR. Complete Genome Sequence of the Type Strain Tepidimonas taiwanensis LMG 22826T, a Thermophilic Alkaline Protease and Polyhydroxyalkanoate Producer. \textit{GENOME BIOLOGY AND EVOLUTION}. OXFORD: OXFORD UNIV PRESS, 2021, vol.~13, No~12, p.~1-5. ISSN~1759-6653. Available from: https://dx.doi.org/10.1093/gbe/evab280.

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