G2/M-checkpoint activation in fasciata1 rescues an aberrant
S-phase checkpoint but causes genome instability

J 2021

G2/M-checkpoint activation in fasciata1 rescues an aberrant S-phase checkpoint but causes genome instability

EEKHOUT, T., Martina DVOŘÁČKOVÁ, J.A.P. GARCIA, Martina NEŠPOR DADEJOVÁ, P. KALHORZADEH et. al.

Basic information

Original name

G2/M-checkpoint activation in fasciata1 rescues an aberrant S-phase checkpoint but causes genome instability

Authors

EEKHOUT, T., Martina DVOŘÁČKOVÁ (203 Czech Republic, belonging to the institution), J.A.P. GARCIA, Martina NEŠPOR DADEJOVÁ (203 Czech Republic, belonging to the institution), P. KALHORZADEH, H. VAN DEN DAELE, I. VERCAUTEREN, Jiří FAJKUS (203 Czech Republic, guarantor, belonging to the institution) and L. DE VEYLDER

Edition

Plant Physiology, ROCKVILLE, American Society of Plant Physiologists, 2021, 0032-0889

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10611 Plant sciences, botany

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 8.005

RIV identification code

RIV/00216224:14740/21:00119672

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1093/plphys/kiab201

UT WoS

000703922000018

Keywords in English

DNA-DAMAGE RESPONSEDEPENDENT KINASE INHIBITORSASSEMBLY FACTOR-IARABIDOPSIS-THALIANAHOMOLOGOUS RECOMBINATION45S RDNACAF-1ATRREPLICATIONDIFFERENTIATION

Abstract

V originále

The WEE1 and ATM AND RAD3-RELATED (ATR) kinases are important regulators of the plant intra-S-phase checkpoint; consequently, WEE1(KO) and ATR(KO) roots are hypersensitive to replication-inhibitory drugs. Here, we report on a loss-of-function mutant allele of the FASCIATA1 (FAS1) subunit of the chromatin assembly factor 1 (CAF-1) complex that suppresses the phenotype of WEE1- or ATR-deficient Arabidopsis (Arabidopsis thaliana) plants. We demonstrate that lack of FAS1 activity results in the activation of an ATAXIA TELANGIECTASIA MUTATED (ATM)- and SUPPRESSOR OF GAMMA-RESPONSE 1 (SOG1)-mediated G2/M-arrest that renders the ATR and WEE1 checkpoint regulators redundant. This ATM activation accounts for the telomere erosion and loss of ribosomal DNA that are described for fast plants. Knocking out SOG1 in the fast weel background restores replication stress sensitivity, demonstrating that SOG1 is an important secondary checkpoint regulator in plants that fail to activate the intra-S-phase checkpoint.

Links

GJ19-11880Y, research and development project

Name: Chromatinové prostředí indukované genotoxickými látkami

Investor: Czech Science Foundation, Chromatin environment induced by genotoxic agents

GX20-01331X, research and development project

Name: Biogeneze a evoluce telomerázy u rostlin

Investor: Czech Science Foundation

LTC18048, research and development project

Name: Prostorové uspořádání významných jaderných domén a jejich proteinových složek

Investor: Ministry of Education, Youth and Sports of the CR, Spatial organisation of key nuclear domains and their protein components, INTER-COST

LTC20003, research and development project

Name: Dynamics of telomeres and rDNA loci in plant cell nuclei

Investor: Ministry of Education, Youth and Sports of the CR, INTER-COST

Citovat

EEKHOUT, T., Martina DVOŘÁČKOVÁ, J.A.P. GARCIA, Martina NEŠPOR DADEJOVÁ, P. KALHORZADEH, H. VAN DEN DAELE, I. VERCAUTEREN, Jiří FAJKUS and L. DE VEYLDER. G2/M-checkpoint activation in fasciata1 rescues an aberrant S-phase checkpoint but causes genome instability. Plant Physiology. ROCKVILLE: American Society of Plant Physiologists, 2021, vol. 186, No 4, p. 1893-1907. ISSN 0032-0889. Available from: https://dx.doi.org/10.1093/plphys/kiab201.

@article{1837260,
   author = {Eekhout, T. and Dvořáčková, Martina and Garcia, J.A.P. and Nešpor Dadejová, Martina and Kalhorzadeh, P. and Van den Daele, H. and Vercauteren, I. and Fajkus, Jiří and De Veylder, L.},
   article_location = {ROCKVILLE},
   article_number = {4},
   doi = {http://dx.doi.org/10.1093/plphys/kiab201},
   keywords = {DNA-DAMAGE RESPONSEDEPENDENT KINASE INHIBITORSASSEMBLY FACTOR-IARABIDOPSIS-THALIANAHOMOLOGOUS RECOMBINATION45S RDNACAF-1ATRREPLICATIONDIFFERENTIATION},
   language = {eng},
   issn = {0032-0889},
   journal = {Plant Physiology},
   title = {G2/M-checkpoint activation in fasciata1 rescues an aberrant S-phase checkpoint but causes genome instability},
   url = {https://academic.oup.com/plphys/article/186/4/1893/6270794},
   volume = {186},
   year = {2021}
}

TY  - JOUR
ID  - 1837260
AU  - Eekhout, T. - Dvořáčková, Martina - Garcia, J.A.P. - Nešpor Dadejová, Martina - Kalhorzadeh, P. - Van den Daele, H. - Vercauteren, I. - Fajkus, Jiří - De Veylder, L.
PY  - 2021
TI  - G2/M-checkpoint activation in fasciata1 rescues an aberrant S-phase checkpoint but causes genome instability
JF  - Plant Physiology
VL  - 186
IS  - 4
SP  - 1893-1907
EP  - 1893-1907
PB  - American Society of Plant Physiologists
SN  - 00320889
KW  - DNA-DAMAGE RESPONSEDEPENDENT KINASE INHIBITORSASSEMBLY FACTOR-IARABIDOPSIS-THALIANAHOMOLOGOUS RECOMBINATION45S RDNACAF-1ATRREPLICATIONDIFFERENTIATION
UR  - https://academic.oup.com/plphys/article/186/4/1893/6270794
N2  - The WEE1 and ATM AND RAD3-RELATED (ATR) kinases are important regulators of the plant intra-S-phase checkpoint; consequently, WEE1(KO) and ATR(KO) roots are hypersensitive to replication-inhibitory drugs. Here, we report on a loss-of-function mutant allele of the FASCIATA1 (FAS1) subunit of the chromatin assembly factor 1 (CAF-1) complex that suppresses the phenotype of WEE1- or ATR-deficient Arabidopsis (Arabidopsis thaliana) plants. We demonstrate that lack of FAS1 activity results in the activation of an ATAXIA TELANGIECTASIA MUTATED (ATM)- and SUPPRESSOR OF GAMMA-RESPONSE 1 (SOG1)-mediated G2/M-arrest that renders the ATR and WEE1 checkpoint regulators redundant. This ATM activation accounts for the telomere erosion and loss of ribosomal DNA that are described for fast plants. Knocking out SOG1 in the fast weel background restores replication stress sensitivity, demonstrating that SOG1 is an important secondary checkpoint regulator in plants that fail to activate the intra-S-phase checkpoint.
ER  -

EEKHOUT, T., Martina DVOŘÁČKOVÁ, J.A.P. GARCIA, Martina NEŠPOR DADEJOVÁ, P. KALHORZADEH, H. VAN DEN DAELE, I. VERCAUTEREN, Jiří FAJKUS and L. DE VEYLDER. G2/M-checkpoint activation in fasciata1 rescues an aberrant S-phase checkpoint but causes genome instability. \textit{Plant Physiology}. ROCKVILLE: American Society of Plant Physiologists, 2021, vol.~186, No~4, p.~1893-1907. ISSN~0032-0889. Available from: https://dx.doi.org/10.1093/plphys/kiab201.

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