Demonstration of the role of cell wall homeostasis in
Staphylococcus aureus growth and the action of bactericidal
antibiotics

J 2021

Demonstration of the role of cell wall homeostasis in Staphylococcus aureus growth and the action of bactericidal antibiotics

SALAMAGA, B.; L.Y. KONG; L. PASQUINA-LEMONCHE; L. LAFAGE; M.V. ZUR MUHLEN et. al.

Základní údaje

Originální název

Demonstration of the role of cell wall homeostasis in Staphylococcus aureus growth and the action of bactericidal antibiotics

Autoři

SALAMAGA, B.; L.Y. KONG; L. PASQUINA-LEMONCHE; L. LAFAGE; M.V. ZUR MUHLEN; J.F. GIBSON; Danyil GRYBCHUK (804 Ukrajina, domácí); A.K. TOOKE; V. PANCHAL; E.J. CULP; E. TATHAM; M.E. O KANE; T.E. CATLEY; S.A. RENSHAW; G.D. WRIGHT; Pavel PLEVKA (203 Česká republika, garant, domácí); P.A. BULLOUGH; A.D. HAN; J.K. HOBBS a S.J. FOSTER

Vydání

Proceedings of the National Academy of Sciences of the United States of America, WASHINGTON, NATL ACAD SCIENCES, 2021, 0027-8424

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 12.779

Kód RIV

RIV/00216224:14740/21:00124299

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1073/pnas.2106022118

UT WoS

000720888300023

EID Scopus

2-s2.0-85119274471

Klíčová slova anglicky

peptidoglycan; antibiotics; cell wall; vancomycin; methicillin

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 26. 2. 2022 13:39, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

Bacterial cell wall peptidoglycan is essential, maintaining both cellular integrity and morphology, in the face of internal turgor pressure. Peptidoglycan synthesis is important, as it is targeted by cell wall antibiotics, including methicillin and vancomycin. Here, we have used the major human pathogen Staphylococcus aureus to elucidate both the cell wall dynamic processes essential for growth (life) and the bactericidal effects of cell wall antibiotics (death) based on the principle of coordinated peptidoglycan synthesis and hydrolysis. The death of S. aureus due to depletion of the essential, two-component and positive regulatory system for peptidoglycan hydrolase activity (WaIKR) is prevented by addition of otherwise bactericidal cell wall antibiotics, resulting in stasis. In contrast, cell wall antibiotics kill via the activity of peptidoglycan hydrolases in the absence of concomitant synthesis. Both methicillin and vancomycin treatment lead to the appearance of perforating holes throughout the cell wall due to peptidoglycan hydrolases. Methicillin alone also results in plasmolysis and misshapen septa with the involvement of the major peptidoglycan hydrolase Atl, a process that is inhibited by vancomycin. The bactericidal effect of vancomycin involves the peptidoglycan hydrolase Sag B. In the presence of cell wall antibiotics, the inhibition of peptidoglycan hydrolase activity using the inhibitor complestatin results in reduced killing, while, conversely, the deregulation of hydrolase activity via loss of wall teichoic acids increases the death rate. For S. aureus, the independent regulation of cell wall synthesis and hydrolysis can lead to cell growth, death, or stasis, with implications for the development of new control regimes for this important pathogen.

Citovat

SALAMAGA, B.; L.Y. KONG; L. PASQUINA-LEMONCHE; L. LAFAGE; M.V. ZUR MUHLEN; J.F. GIBSON; Danyil GRYBCHUK; A.K. TOOKE; V. PANCHAL; E.J. CULP; E. TATHAM; M.E. O KANE; T.E. CATLEY; S.A. RENSHAW; G.D. WRIGHT; Pavel PLEVKA; P.A. BULLOUGH; A.D. HAN; J.K. HOBBS a S.J. FOSTER. Demonstration of the role of cell wall homeostasis in Staphylococcus aureus growth and the action of bactericidal antibiotics. Proceedings of the National Academy of Sciences of the United States of America. WASHINGTON: NATL ACAD SCIENCES, 2021, roč. 118, č. 44, s. „e2106022118“, 8 s. ISSN 0027-8424. Dostupné z: https://dx.doi.org/10.1073/pnas.2106022118.

@article{1837278,
   author = {Salamaga, B. and Kong, L.Y. and PasquinaandLemonche, L. and Lafage, L. and zur Muhlen, M.V. and Gibson, J.F. and Grybchuk, Danyil and Tooke, A.K. and Panchal, V. and Culp, E.J. and Tatham, E. and O Kane, M.E. and Catley, T.E. and Renshaw, S.A. and Wright, G.D. and Plevka, Pavel and Bullough, P.A. and Han, A.D. and Hobbs, J.K. and Foster, S.J.},
   article_location = {WASHINGTON},
   article_number = {44},
   doi = {http://dx.doi.org/10.1073/pnas.2106022118},
   keywords = {peptidoglycan; antibiotics; cell wall; vancomycin; methicillin},
   language = {eng},
   issn = {0027-8424},
   journal = {Proceedings of the National Academy of Sciences of the United States of America},
   title = {Demonstration of the role of cell wall homeostasis in Staphylococcus aureus growth and the action of bactericidal antibiotics},
   url = {https://www.pnas.org/content/118/44/e2106022118},
   volume = {118},
   year = {2021}
}

TY  - JOUR
ID  - 1837278
AU  - Salamaga, B. - Kong, L.Y. - Pasquina-Lemonche, L. - Lafage, L. - zur Muhlen, M.V. - Gibson, J.F. - Grybchuk, Danyil - Tooke, A.K. - Panchal, V. - Culp, E.J. - Tatham, E. - O Kane, M.E. - Catley, T.E. - Renshaw, S.A. - Wright, G.D. - Plevka, Pavel - Bullough, P.A. - Han, A.D. - Hobbs, J.K. - Foster, S.J.
PY  - 2021
TI  - Demonstration of the role of cell wall homeostasis in Staphylococcus aureus growth and the action of bactericidal antibiotics
JF  - Proceedings of the National Academy of Sciences of the United States of America
VL  - 118
IS  - 44
SP  - „e2106022118“
EP  - „e2106022118“
PB  - NATL ACAD SCIENCES
SN  - 00278424
KW  - peptidoglycan
KW  - antibiotics
KW  - cell wall
KW  - vancomycin
KW  - methicillin
UR  - https://www.pnas.org/content/118/44/e2106022118
N2  - Bacterial cell wall peptidoglycan is essential, maintaining both cellular integrity and morphology, in the face of internal turgor pressure. Peptidoglycan synthesis is important, as it is targeted by cell wall antibiotics, including methicillin and vancomycin. Here, we have used the major human pathogen Staphylococcus aureus to elucidate both the cell wall dynamic processes essential for growth (life) and the bactericidal effects of cell wall antibiotics (death) based on the principle of coordinated peptidoglycan synthesis and hydrolysis. The death of S. aureus due to depletion of the essential, two-component and positive regulatory system for peptidoglycan hydrolase activity (WaIKR) is prevented by addition of otherwise bactericidal cell wall antibiotics, resulting in stasis. In contrast, cell wall antibiotics kill via the activity of peptidoglycan hydrolases in the absence of concomitant synthesis. Both methicillin and vancomycin treatment lead to the appearance of perforating holes throughout the cell wall due to peptidoglycan hydrolases. Methicillin alone also results in plasmolysis and misshapen septa with the involvement of the major peptidoglycan hydrolase Atl, a process that is inhibited by vancomycin. The bactericidal effect of vancomycin involves the peptidoglycan hydrolase Sag B. In the presence of cell wall antibiotics, the inhibition of peptidoglycan hydrolase activity using the inhibitor complestatin results in reduced killing, while, conversely, the deregulation of hydrolase activity via loss of wall teichoic acids increases the death rate. For S. aureus, the independent regulation of cell wall synthesis and hydrolysis can lead to cell growth, death, or stasis, with implications for the development of new control regimes for this important pathogen.
ER  -

SALAMAGA, B.; L.Y. KONG; L. PASQUINA-LEMONCHE; L. LAFAGE; M.V. ZUR MUHLEN; J.F. GIBSON; Danyil GRYBCHUK; A.K. TOOKE; V. PANCHAL; E.J. CULP; E. TATHAM; M.E. O KANE; T.E. CATLEY; S.A. RENSHAW; G.D. WRIGHT; Pavel PLEVKA; P.A. BULLOUGH; A.D. HAN; J.K. HOBBS a S.J. FOSTER. Demonstration of the role of cell wall homeostasis in Staphylococcus aureus growth and the action of bactericidal antibiotics. \textit{Proceedings of the National Academy of Sciences of the United States of America}. WASHINGTON: NATL ACAD SCIENCES, 2021, roč.~118, č.~44, s.~„e2106022118“, 8 s. ISSN~0027-8424. Dostupné z: https://dx.doi.org/10.1073/pnas.2106022118.

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