B1-type cyclins control microtubule organization during cell
division in Arabidopsis

J 2022

B1-type cyclins control microtubule organization during cell division in Arabidopsis

MOTTA, M.R., X. ZHAO, M. PASTUGLIA, K. BELCRAM, F. ROODBARKELARI et. al.

Basic information

Original name

B1-type cyclins control microtubule organization during cell division in Arabidopsis

Authors

MOTTA, M.R., X. ZHAO, M. PASTUGLIA, K. BELCRAM, F. ROODBARKELARI, M. KOMAKI, H. HARASHIMA, S. KOMAKI, M. KUMAR, P. BULANKOVA, M. HEESE, Karel ŘÍHA (203 Czech Republic, guarantor, belonging to the institution), D. BOUCHEZ and A. SCHNITTGER

Edition

EMBO reports, Hoboken, Wiley-Blackwell, 2022, 1469-221X

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10611 Plant sciences, botany

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 7.700

RIV identification code

RIV/00216224:14740/22:00125443

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.15252/embr.202153995

UT WoS

000728233300001

Keywords in English

CDK; CYCB1; endosperm; microtubule nucleation; mitosis

Abstract

V originále

Flowering plants contain a large number of cyclin families, each containing multiple members, most of which have not been characterized to date. Here, we analyzed the role of the B1 subclass of mitotic cyclins in cell cycle control during Arabidopsis development. While we reveal CYCB1;5 to be a pseudogene, the remaining four members were found to be expressed in dividing cells. Mutant analyses showed a complex pattern of overlapping, development-specific requirements of B1-type cyclins with CYCB1;2 playing a central role. The double mutant cycb1;1 cycb1;2 is severely compromised in growth, yet viable beyond the seedling stage, hence representing a unique opportunity to study the function of B1-type cyclin activity at the organismic level. Immunolocalization of microtubules in cycb1;1 cycb1;2 and treating mutants with the microtubule drug oryzalin revealed a key role of B1-type cyclins in orchestrating mitotic microtubule networks. Subsequently, we identified the GAMMA-TUBULIN COMPLEX PROTEIN 3-INTERACTING PROTEIN 1 (GIP1/MOZART) as an in vitro substrate of B1-type cyclin complexes and further genetic analyses support a potential role in the regulation of GIP1 by CYCB1s.

Links

EF15_003/0000479, research and development project

Name: Regulace rostlinné meiózy

Citovat

MOTTA, M.R., X. ZHAO, M. PASTUGLIA, K. BELCRAM, F. ROODBARKELARI, M. KOMAKI, H. HARASHIMA, S. KOMAKI, M. KUMAR, P. BULANKOVA, M. HEESE, Karel ŘÍHA, D. BOUCHEZ and A. SCHNITTGER. B1-type cyclins control microtubule organization during cell division in Arabidopsis. EMBO reports. Hoboken: Wiley-Blackwell, 2022, vol. 23, No 1, p. „e53995“, 18 pp. ISSN 1469-221X. Available from: https://dx.doi.org/10.15252/embr.202153995.

@article{1837293,
   author = {Motta, M.R. and Zhao, X. and Pastuglia, M. and Belcram, K. and Roodbarkelari, F. and Komaki, M. and Harashima, H. and Komaki, S. and Kumar, M. and Bulankova, P. and Heese, M. and Říha, Karel and Bouchez, D. and Schnittger, A.},
   article_location = {Hoboken},
   article_number = {1},
   doi = {http://dx.doi.org/10.15252/embr.202153995},
   keywords = {CDK; CYCB1; endosperm; microtubule nucleation; mitosis},
   language = {eng},
   issn = {1469-221X},
   journal = {EMBO reports},
   title = {B1-type cyclins control microtubule organization during cell division in Arabidopsis},
   url = {https://www.embopress.org/doi/full/10.15252/embr.202153995},
   volume = {23},
   year = {2022}
}

TY  - JOUR
ID  - 1837293
AU  - Motta, M.R. - Zhao, X. - Pastuglia, M. - Belcram, K. - Roodbarkelari, F. - Komaki, M. - Harashima, H. - Komaki, S. - Kumar, M. - Bulankova, P. - Heese, M. - Říha, Karel - Bouchez, D. - Schnittger, A.
PY  - 2022
TI  - B1-type cyclins control microtubule organization during cell division in Arabidopsis
JF  - EMBO reports
VL  - 23
IS  - 1
SP  - „e53995“
EP  - „e53995“
PB  - Wiley-Blackwell
SN  - 1469221X
KW  - CDK
KW  - CYCB1
KW  - endosperm
KW  - microtubule nucleation
KW  - mitosis
UR  - https://www.embopress.org/doi/full/10.15252/embr.202153995
N2  - Flowering plants contain a large number of cyclin families, each containing multiple members, most of which have not been characterized to date. Here, we analyzed the role of the B1 subclass of mitotic cyclins in cell cycle control during Arabidopsis development. While we reveal CYCB1;5 to be a pseudogene, the remaining four members were found to be expressed in dividing cells. Mutant analyses showed a complex pattern of overlapping, development-specific requirements of B1-type cyclins with CYCB1;2 playing a central role. The double mutant cycb1;1 cycb1;2 is severely compromised in growth, yet viable beyond the seedling stage, hence representing a unique opportunity to study the function of B1-type cyclin activity at the organismic level. Immunolocalization of microtubules in cycb1;1 cycb1;2 and treating mutants with the microtubule drug oryzalin revealed a key role of B1-type cyclins in orchestrating mitotic microtubule networks. Subsequently, we identified the GAMMA-TUBULIN COMPLEX PROTEIN 3-INTERACTING PROTEIN 1 (GIP1/MOZART) as an in vitro substrate of B1-type cyclin complexes and further genetic analyses support a potential role in the regulation of GIP1 by CYCB1s.
ER  -

MOTTA, M.R., X. ZHAO, M. PASTUGLIA, K. BELCRAM, F. ROODBARKELARI, M. KOMAKI, H. HARASHIMA, S. KOMAKI, M. KUMAR, P. BULANKOVA, M. HEESE, Karel ŘÍHA, D. BOUCHEZ and A. SCHNITTGER. B1-type cyclins control microtubule organization during cell division in Arabidopsis. \textit{EMBO reports}. Hoboken: Wiley-Blackwell, 2022, vol.~23, No~1, p.~„e53995“, 18 pp. ISSN~1469-221X. Available from: https://dx.doi.org/10.15252/embr.202153995.

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