A hypomorphic allele of telomerase uncovers the minimal
functional length of telomeres in Arabidopsis

J 2021

A hypomorphic allele of telomerase uncovers the minimal functional length of telomeres in Arabidopsis

WATSON, J.M., J. TRIEB, M. TROESTL, K. RENFREW, Terezie MALÍK MANDÁKOVÁ et. al.

Basic information

Original name

A hypomorphic allele of telomerase uncovers the minimal functional length of telomeres in Arabidopsis

Authors

WATSON, J.M., J. TRIEB, M. TROESTL, K. RENFREW, Terezie MALÍK MANDÁKOVÁ (203 Czech Republic, belonging to the institution), Jaroslav FULNEČEK (203 Czech Republic, belonging to the institution), D.E. SHIPPEN and Karel ŘÍHA (203 Czech Republic, guarantor, belonging to the institution)

Edition

Genetics, BETHESDA (USA), GENETICS SOCIETY AMERICA, 2021, 0016-6731

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10603 Genetics and heredity

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.402

RIV identification code

RIV/00216224:14740/21:00119673

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1093/genetics/iyab126

UT WoS

000708515000019

Keywords in English

telomeres; telomerase; genome stability; subtelomere; t-loop

Abstract

V originále

Despite the essential requirement of telomeric DNA for genome stability, the length of telomere tracts between species substantially differs, raising the question of the minimal length of telomeric DNA necessary for proper function. Here, we address this question using a hypomorphic allele of the telomerase catalytic subunit, TERT. We show that although this construct partially restored telomerase activity to a tert mutant, telomeres continued to shorten over several generations, ultimately stabilizing at a bimodal size distribution. Telomeres on two chromosome arms were maintained at a length of 1 kb, while the remaining telomeres were maintained at 400 bp. The longest telomeres identified in this background were also significantly longer in wild-type populations, suggesting cis-acting elements on these arms either promote telomerase processivity or recruitment. Genetically disrupting telomerase processivity in this background resulted in immediate lethality. Thus, telomeres of 400 bp are both necessary and sufficient for Arabidopsis viability. As this length is the estimated minimal length for t-loop formation, our data suggest that telomeres long enough to form a t-loop constitute the minimal functional length.

Links

EF15_003/0000479, research and development project

Name: Regulace rostlinné meiózy

GA18-20134S, research and development project

Name: Organizace interfázních chromosomů a časné meiotické párování chromosomů u brukvovitých

Investor: Czech Science Foundation

GA19-21961S, research and development project

Name: Chemická modulace aktivity Ku komplexu a využití v editaci genomů.

Investor: Czech Science Foundation, Chemical modulation of Ku activity and its use in genome editing

1304130933, interní kód MU

Name: Blunt-ended telomeres: dissecting the novel mechanism of chromosome end protection

Investor: EMBO (European Molecular Biology Organization)

90129, large research infrastructures

Name: Czech-BioImaging II

Citovat

WATSON, J.M., J. TRIEB, M. TROESTL, K. RENFREW, Terezie MALÍK MANDÁKOVÁ, Jaroslav FULNEČEK, D.E. SHIPPEN and Karel ŘÍHA. A hypomorphic allele of telomerase uncovers the minimal functional length of telomeres in Arabidopsis. Genetics. BETHESDA (USA): GENETICS SOCIETY AMERICA, 2021, vol. 219, No 2, p. „iyab126“, 9 pp. ISSN 0016-6731. Available from: https://dx.doi.org/10.1093/genetics/iyab126.

@article{1837299,
   author = {Watson, J.M. and Trieb, J. and Troestl, M. and Renfrew, K. and Malík Mandáková, Terezie and Fulneček, Jaroslav and Shippen, D.E. and Říha, Karel},
   article_location = {BETHESDA (USA)},
   article_number = {2},
   doi = {http://dx.doi.org/10.1093/genetics/iyab126},
   keywords = {telomeres; telomerase; genome stability; subtelomere; t-loop},
   language = {eng},
   issn = {0016-6731},
   journal = {Genetics},
   title = {A hypomorphic allele of telomerase uncovers the minimal functional length of telomeres in Arabidopsis},
   url = {https://academic.oup.com/genetics/article-abstract/219/2/iyab126/6339584?redirectedFrom=fulltext},
   volume = {219},
   year = {2021}
}

TY  - JOUR
ID  - 1837299
AU  - Watson, J.M. - Trieb, J. - Troestl, M. - Renfrew, K. - Malík Mandáková, Terezie - Fulneček, Jaroslav - Shippen, D.E. - Říha, Karel
PY  - 2021
TI  - A hypomorphic allele of telomerase uncovers the minimal functional length of telomeres in Arabidopsis
JF  - Genetics
VL  - 219
IS  - 2
SP  - „iyab126“
EP  - „iyab126“
PB  - GENETICS SOCIETY AMERICA
SN  - 00166731
KW  - telomeres
KW  - telomerase
KW  - genome stability
KW  - subtelomere
KW  - t-loop
UR  - https://academic.oup.com/genetics/article-abstract/219/2/iyab126/6339584?redirectedFrom=fulltext
N2  - Despite the essential requirement of telomeric DNA for genome stability, the length of telomere tracts between species substantially differs, raising the question of the minimal length of telomeric DNA necessary for proper function. Here, we address this question using a hypomorphic allele of the telomerase catalytic subunit, TERT. We show that although this construct partially restored telomerase activity to a tert mutant, telomeres continued to shorten over several generations, ultimately stabilizing at a bimodal size distribution. Telomeres on two chromosome arms were maintained at a length of 1 kb, while the remaining telomeres were maintained at 400 bp. The longest telomeres identified in this background were also significantly longer in wild-type populations, suggesting cis-acting elements on these arms either promote telomerase processivity or recruitment. Genetically disrupting telomerase processivity in this background resulted in immediate lethality. Thus, telomeres of 400 bp are both necessary and sufficient for Arabidopsis viability. As this length is the estimated minimal length for t-loop formation, our data suggest that telomeres long enough to form a t-loop constitute the minimal functional length.
ER  -

WATSON, J.M., J. TRIEB, M. TROESTL, K. RENFREW, Terezie MALÍK MANDÁKOVÁ, Jaroslav FULNEČEK, D.E. SHIPPEN and Karel ŘÍHA. A hypomorphic allele of telomerase uncovers the minimal functional length of telomeres in Arabidopsis. \textit{Genetics}. BETHESDA (USA): GENETICS SOCIETY AMERICA, 2021, vol.~219, No~2, p.~„iyab126“, 9 pp. ISSN~0016-6731. Available from: https://dx.doi.org/10.1093/genetics/iyab126.

Detailed Information on Publication Record