a 2021

Ticks from wildlife animals in South Africa: molecular detection of Rickettsia sp

KAŠPÁRKOVÁ, Nikola, Eva BÁRTOVÁ, A. HALAJIAN and Alena ŽÁKOVSKÁ

Basic information

Original name

Ticks from wildlife animals in South Africa: molecular detection of Rickettsia sp

Name in Czech

Klíšťata z volně žijících zvířat v Jižní Africe: molekulární detekce Rickettsia sp

Name (in English)

Ticks from wildlife animals in South Africa: molecular detection of Rickettsia sp

Authors

KAŠPÁRKOVÁ, Nikola (203 Czech Republic), Eva BÁRTOVÁ (203 Czech Republic), A. HALAJIAN (203 Czech Republic) and Alena ŽÁKOVSKÁ (203 Czech Republic, belonging to the institution)

Edition

Tomáškovy dny 2021 mladých mikrobiologů, 2021

Other information

Language

Czech

Type of outcome

Konferenční abstrakt

Field of Study

10606 Microbiology

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

RIV identification code

RIV/00216224:14310/21:00124320

Organization unit

Faculty of Science

ISBN

978-80-210-9882-4

Keywords (in Czech)

Klíšťata volně žijící zvířaat Jižní Afrika molekulární detekce Rickettsia sp

Keywords in English

Wildlife ticks South Africa molecular detection Rickettsia sp
Změněno: 28/2/2022 14:06, doc. RNDr. Alena Žákovská, Ph.D.

Abstract

V originále

Members of the genus Rickettsia are small, obligate intracellular, Gram-negative bacteria that are distributed throughout the world. The infection can be transmitted through arthropod bites and can cause health problems to the animals and humans, because it is widespread tick-borne disease zoonoses. The aim of the study was to detect Rickettsia sp. in ticks from South Africa. Ticks were collected during the years 2012-2019 in six provinces of South Africa including Limpopo, Mpumalanga, Free State, Northern Cape, North West, and Gauteng Province. Ticks were taken from dead animals (most often because of a collision with a car). In total, 2003 ticks (154 females, 778 males, 454 nymphs, and 617 larvae) were collected and divided into 854 samples. The DNA from ticks was isolated by NucleoSpin Tissue kit to detect Rickettsia sp. by single PCR.

In English

Members of the genus Rickettsia are small, obligate intracellular, Gram-negative bacteria that are distributed throughout the world. The infection can be transmitted through arthropod bites and can cause health problems to the animals and humans, because it is widespread tick-borne diseases zoonoses. The aim of the study was to detect Rickettsia sp. in ticks from South Africa. Ticks were collected during the years 2012-2019 in six provinces of South Africa including Limpopo, Mpumalanga, Free State, Northern Cape, North West, and Gauteng Province. Ticks were taken from dead animals (most often because of a collision with a car). In total, 2003 ticks (154 females, 778 males, 454 nymphs, and 617 larvae) were collected and divided into 854 samples. The DNA from ticks was isolated by NucleoSpin Tissue kit to detect Rickettsia sp. by single PCR.