Molecular detection of Rickettsia sp. in ticks from wildlife animals living in six provinces of South Africa

KAŠPÁRKOVÁ, Nikola, Eva BÁRTOVÁ, A. HALAJIAN and Alena ŽÁKOVSKÁ. Molecular detection of Rickettsia sp. in ticks from wildlife animals living in six provinces of South Africa. 2021. ISBN 978-80-7305-851-7.

Basic information

• Original name: Molecular detection of Rickettsia sp. in ticks from wildlife animals living in six provinces of South Africa
• Name in Czech: Molekulární detekce Rickettsia sp. u klíšťat od volně žijících zvířat žijících v šest provincií Jižní Afriky
• Name (in English): Molecular detection of Rickettsia sp. in ticks from wildlife animals living in six provinces of South Africa
• Authors: KAŠPÁRKOVÁ, Nikola, Eva BÁRTOVÁ, A. HALAJIAN and Alena ŽÁKOVSKÁ.
• Edition: 2021.

Other information

• Original language: Czech
• Type of outcome: Conference abstract
• Field of Study: 10606 Microbiology
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• Organization unit: Faculty of Education
• ISBN: 978-80-7305-851-7
• Keywords (in Czech): Klíšťata molekulární detekce Rickettsia sp
• Keywords in English: Wildlife ticks molecular detection Rickettsia sp

Abstract

In total, 854 ticks of different species from six provinces of South Africa were screened for the presence of Rickettsia sp. by a single PCR assay targeting the gltA gene using the primers CS 78 and CS 323. The DNA of Rickettsia sp. was detected in 144 samples (17 %) with 18% in females, 13% in males, 26 % in nymphs (26%) and 18% in larvae. The highest prevalence of Rickettsia sp. was in Free state Province (50%) and in ticks collected in spring (22%) and autumn (19%). Positive samples will be sent for sequencing for accurate identification. The results of this study bring new knowledge about the prevalence of this pathogen in ticks in six provinces of South Africa and may be the basis for more extensive research in South Africa.

Abstract (in English)

In total, 854 ticks of different species from six provinces of South Africa were screened for the presence of Rickettsia sp. by a single PCR assay targeting the gltA gene using the primers CS 78 and CS 323. The DNA of Rickettsia sp. was detected in 144 samples (17 %) with 18% in females, 13% in males, 26 % in nymphs (26%) and 18% in larvae. The highest prevalence of Rickettsia sp. was in Free state Province (50%) and in ticks collected in spring (22%) and autumn (19%). Positive samples will be sent for sequencing for accurate identification. The results of this study bring new knowledge about the prevalence of this pathogen in ticks in six provinces of South Africa and may be the basis for more extensive research in South Africa.

Links

• MUNI/A/1403/2020, interní kód MU: Name: Podpora výzkumné činnosti studentů v oblasti fyziologie, vývojové biologie a imunologie živočichů 2021 (Acronym: Podpora studentů)

Investor: Masaryk University