Crucial factors governing the electrochemical impedance on
protein-modified surfaces

J 2021

Crucial factors governing the electrochemical impedance on protein-modified surfaces

SOPOUŠEK, Jakub, Jakub VĚŽNÍK, Josef HOUSER, Petr SKLÁDAL, Karel LACINA et. al.

Basic information

Original name

Crucial factors governing the electrochemical impedance on protein-modified surfaces

Authors

SOPOUŠEK, Jakub (203 Czech Republic, belonging to the institution), Jakub VĚŽNÍK (203 Czech Republic, belonging to the institution), Josef HOUSER (203 Czech Republic, belonging to the institution), Petr SKLÁDAL (203 Czech Republic, belonging to the institution) and Karel LACINA (203 Czech Republic, guarantor, belonging to the institution)

Edition

Electrochimica Acta, OXFORD, Elsevier, 2021, 0013-4686

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10405 Electrochemistry

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 7.336

RIV identification code

RIV/00216224:14740/21:00119677

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1016/j.electacta.2021.138616

UT WoS

000670355000003

Keywords in English

Electrochemical impedance spectroscopy; Polystyrene nanoparticles; Polyacrylic acid; Bovine serum albumin; Cationized bovine serum albumin; Ionic strength; Ionic charge

Abstract

V originále

Three different electrode surfaces - two nanoporous and one modified with polyelectrolyte - provided with positively and negatively charged model proteins were studied at different conditions to determine characteristics of electrochemical impedance spectroscopy in biosensing. The impedance response in the presence of [Fe(CN)(6)(4-/3-) was simultaneously influenced by two effects - ionic attraction/repulsion and steric hindrance of protein components. The ionic interactions dominated at low ionic strength and at low concentrations of redox probe. On the other hand, the steric hindrance of immobilized proteins affecting mass transfer towards the electrode gained impact gradually with the increasing ionic strength and with the concentration of the redox-active component. The simultaneous effect of these two variables can lead to confusing results which are often misinterpreted when evaluating results from impedimetric affinity biosensors. (C) 2021 Elsevier Ltd. All rights reserved.

Links

EF19_073/0016943, research and development project

Name: Interní grantová agentura Masarykovy univerzity

GJ19-16273Y, research and development project

Name: ROS-indukované uvolňování léčiv založené na interakcích ferocenylboronátů

Investor: Czech Science Foundation, ROS-Induced Drug Release Based on Interactions of Ferrocenylboronates

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

90127, large research infrastructures

Name: CIISB II

Citovat

SOPOUŠEK, Jakub, Jakub VĚŽNÍK, Josef HOUSER, Petr SKLÁDAL and Karel LACINA. Crucial factors governing the electrochemical impedance on protein-modified surfaces. Electrochimica Acta. OXFORD: Elsevier, 2021, vol. 388, AUG, p. 138616-138622. ISSN 0013-4686. Available from: https://dx.doi.org/10.1016/j.electacta.2021.138616.

@article{1838000,
   author = {Sopoušek, Jakub and Věžník, Jakub and Houser, Josef and Skládal, Petr and Lacina, Karel},
   article_location = {OXFORD},
   article_number = {AUG},
   doi = {http://dx.doi.org/10.1016/j.electacta.2021.138616},
   keywords = {Electrochemical impedance spectroscopy; Polystyrene nanoparticles; Polyacrylic acid; Bovine serum albumin; Cationized bovine serum albumin; Ionic strength; Ionic charge},
   language = {eng},
   issn = {0013-4686},
   journal = {Electrochimica Acta},
   title = {Crucial factors governing the electrochemical impedance on protein-modified surfaces},
   url = {https://www.sciencedirect.com/science/article/pii/S0013468621009063?via%3Dihub},
   volume = {388},
   year = {2021}
}

TY  - JOUR
ID  - 1838000
AU  - Sopoušek, Jakub - Věžník, Jakub - Houser, Josef - Skládal, Petr - Lacina, Karel
PY  - 2021
TI  - Crucial factors governing the electrochemical impedance on protein-modified surfaces
JF  - Electrochimica Acta
VL  - 388
IS  - AUG
SP  - 138616
EP  - 138616
PB  - Elsevier
SN  - 00134686
KW  - Electrochemical impedance spectroscopy
KW  - Polystyrene nanoparticles
KW  - Polyacrylic acid
KW  - Bovine serum albumin
KW  - Cationized bovine serum albumin
KW  - Ionic strength
KW  - Ionic charge
UR  - https://www.sciencedirect.com/science/article/pii/S0013468621009063?via%3Dihub
N2  - Three different electrode surfaces - two nanoporous and one modified with polyelectrolyte - provided with positively and negatively charged model proteins were studied at different conditions to determine characteristics of electrochemical impedance spectroscopy in biosensing. The impedance response in the presence of [Fe(CN)(6)(4-/3-) was simultaneously influenced by two effects - ionic attraction/repulsion and steric hindrance of protein components. The ionic interactions dominated at low ionic strength and at low concentrations of redox probe. On the other hand, the steric hindrance of immobilized proteins affecting mass transfer towards the electrode gained impact gradually with the increasing ionic strength and with the concentration of the redox-active component. The simultaneous effect of these two variables can lead to confusing results which are often misinterpreted when evaluating results from impedimetric affinity biosensors. (C) 2021 Elsevier Ltd. All rights reserved.
ER  -

SOPOUŠEK, Jakub, Jakub VĚŽNÍK, Josef HOUSER, Petr SKLÁDAL and Karel LACINA. Crucial factors governing the electrochemical impedance on protein-modified surfaces. \textit{Electrochimica Acta}. OXFORD: Elsevier, 2021, vol.~388, AUG, p.~138616-138622. ISSN~0013-4686. Available from: https://dx.doi.org/10.1016/j.electacta.2021.138616.

Detailed Information on Publication Record