Thick nanoporous matrices of polystyrene nanoparticles and
their potential for electrochemical biosensing

J 2021

Thick nanoporous matrices of polystyrene nanoparticles and their potential for electrochemical biosensing

SOPOUŠEK, Jakub, Josef HUMLÍČEK, Antonín HLAVÁČEK, Veronika HORÁČKOVÁ, Petr SKLÁDAL et. al.

Basic information

Original name

Thick nanoporous matrices of polystyrene nanoparticles and their potential for electrochemical biosensing

Authors

SOPOUŠEK, Jakub (203 Czech Republic), Josef HUMLÍČEK (203 Czech Republic, belonging to the institution), Antonín HLAVÁČEK (203 Czech Republic), Veronika HORÁČKOVÁ (203 Czech Republic), Petr SKLÁDAL (203 Czech Republic, belonging to the institution) and Karel LACINA (203 Czech Republic, guarantor, belonging to the institution)

Edition

Electrochimica Acta, OXFORD, Elsevier, 2021, 0013-4686

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10405 Electrochemistry

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 7.336

RIV identification code

RIV/00216224:14740/21:00119678

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1016/j.electacta.2020.137607

UT WoS

000609069000013

Keywords in English

Nanopores; Nanoparticles; Multilayers; Thin film interference; Electrochemical impedance; Biosensing

Abstract

V originále

Solid-state nanopores with diameter in units of nanometer can be formed by assembling spherical nanoparticles in a dense arrangement. In the current work, the properties of multi-layered highly ordered assemblies of polystyrene nanoparticles were studied, and their feasibility for electrochemical biosensing was probed. These thick matrices were built using a step-by-step deposition technique. Each individual layer of NPs exhibited distinct color which was caused by the thin film interference effect (a color of specific wavelength was characteristic for matrix of specific thickness). The electrochemical characteristics of matrices were investigated with impedance spectroscopy. The impedance spectra of multi-layered matrices exhibited formation of an additional semicircle (RC component additional to the one in a common Randles equivalent circuit). Further, model biosensing based on nanopore blocking was performed using human serum albumin as an antigen and the corresponding monoclonal antibody as an analyte (serology format). Resulting data disfavored the direct employment of the multi-layered matrices for biosensing purposes as the efficiency decreased with increasing thickness of matrices. However, the data revealed highly valuable information about the diffusion and redox processes in the thick nanoporous matrices. (C) 2020 Elsevier Ltd. All rights reserved.

Links

GJ19-16273Y, research and development project

Name: ROS-indukované uvolňování léčiv založené na interakcích ferocenylboronátů

Investor: Czech Science Foundation, ROS-Induced Drug Release Based on Interactions of Ferrocenylboronates

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

90127, large research infrastructures

Name: CIISB II

Citovat

SOPOUŠEK, Jakub, Josef HUMLÍČEK, Antonín HLAVÁČEK, Veronika HORÁČKOVÁ, Petr SKLÁDAL and Karel LACINA. Thick nanoporous matrices of polystyrene nanoparticles and their potential for electrochemical biosensing. Electrochimica Acta. OXFORD: Elsevier, 2021, vol. 368, FEB, p. 137607-137615. ISSN 0013-4686. Available from: https://dx.doi.org/10.1016/j.electacta.2020.137607.

@article{1838037,
   author = {Sopoušek, Jakub and Humlíček, Josef and Hlaváček, Antonín and Horáčková, Veronika and Skládal, Petr and Lacina, Karel},
   article_location = {OXFORD},
   article_number = {FEB},
   doi = {http://dx.doi.org/10.1016/j.electacta.2020.137607},
   keywords = {Nanopores; Nanoparticles; Multilayers; Thin film interference; Electrochemical impedance; Biosensing},
   language = {eng},
   issn = {0013-4686},
   journal = {Electrochimica Acta},
   title = {Thick nanoporous matrices of polystyrene nanoparticles and their potential for electrochemical biosensing},
   url = {https://www.sciencedirect.com/science/article/pii/S0013468620320004?via%3Dihub},
   volume = {368},
   year = {2021}
}

TY  - JOUR
ID  - 1838037
AU  - Sopoušek, Jakub - Humlíček, Josef - Hlaváček, Antonín - Horáčková, Veronika - Skládal, Petr - Lacina, Karel
PY  - 2021
TI  - Thick nanoporous matrices of polystyrene nanoparticles and their potential for electrochemical biosensing
JF  - Electrochimica Acta
VL  - 368
IS  - FEB
SP  - 137607
EP  - 137607
PB  - Elsevier
SN  - 00134686
KW  - Nanopores
KW  - Nanoparticles
KW  - Multilayers
KW  - Thin film interference
KW  - Electrochemical impedance
KW  - Biosensing
UR  - https://www.sciencedirect.com/science/article/pii/S0013468620320004?via%3Dihub
N2  - Solid-state nanopores with diameter in units of nanometer can be formed by assembling spherical nanoparticles in a dense arrangement. In the current work, the properties of multi-layered highly ordered assemblies of polystyrene nanoparticles were studied, and their feasibility for electrochemical biosensing was probed. These thick matrices were built using a step-by-step deposition technique. Each individual layer of NPs exhibited distinct color which was caused by the thin film interference effect (a color of specific wavelength was characteristic for matrix of specific thickness). The electrochemical characteristics of matrices were investigated with impedance spectroscopy. The impedance spectra of multi-layered matrices exhibited formation of an additional semicircle (RC component additional to the one in a common Randles equivalent circuit). Further, model biosensing based on nanopore blocking was performed using human serum albumin as an antigen and the corresponding monoclonal antibody as an analyte (serology format). Resulting data disfavored the direct employment of the multi-layered matrices for biosensing purposes as the efficiency decreased with increasing thickness of matrices. However, the data revealed highly valuable information about the diffusion and redox processes in the thick nanoporous matrices. (C) 2020 Elsevier Ltd. All rights reserved.
ER  -

SOPOUŠEK, Jakub, Josef HUMLÍČEK, Antonín HLAVÁČEK, Veronika HORÁČKOVÁ, Petr SKLÁDAL and Karel LACINA. Thick nanoporous matrices of polystyrene nanoparticles and their potential for electrochemical biosensing. \textit{Electrochimica Acta}. OXFORD: Elsevier, 2021, vol.~368, FEB, p.~137607-137615. ISSN~0013-4686. Available from: https://dx.doi.org/10.1016/j.electacta.2020.137607.

Detailed Information on Publication Record