Detailed Information on Publication Record
2021
Evolution of plant telomerase RNAs: farther to the past, deeper to the roots
FAJKUS, Petr, Agata Magdalena KILAR, A.D.L. NELSON, M. HOLA, V. PESKA et. al.Basic information
Original name
Evolution of plant telomerase RNAs: farther to the past, deeper to the roots
Authors
FAJKUS, Petr (203 Czech Republic, belonging to the institution), Agata Magdalena KILAR (616 Poland, belonging to the institution), A.D.L. NELSON, M. HOLA, V. PESKA, Ivana GOFFOVÁ (703 Slovakia, belonging to the institution), Miloslava FOJTOVÁ (203 Czech Republic, belonging to the institution), Dagmar ZACHOVÁ (203 Czech Republic, belonging to the institution), Jana FULNEČKOVÁ (203 Czech Republic) and Jiří FAJKUS (203 Czech Republic, guarantor, belonging to the institution)
Edition
Nucleic acids research, Oxford, Oxford University Press, 2021, 0305-1048
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10608 Biochemistry and molecular biology
Country of publisher
United Kingdom of Great Britain and Northern Ireland
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 19.160
RIV identification code
RIV/00216224:14740/21:00119679
Organization unit
Central European Institute of Technology
UT WoS
000685211300038
Keywords in English
TEMPLATE-BOUNDARY DEFINITION; SECONDARY STRUCTURE; POLYMERASE-III; SNRNA GENES; SEQUENCE; IDENTIFICATION; PROMOTER; PSEUDOKNOT; ELEMENT; ARABIDOPSIS
Tags
International impact, Reviewed
Změněno: 15/10/2024 14:07, Ing. Martina Blahová
Abstract
V originále
The enormous sequence heterogeneity of telomerase RNA (TR) subunits has thus far complicated their characterization in a wider phylogenetic range. Our recent finding that land plant TRs are, similarly to known ciliate TRs, transcribed by RNA polymerase III and under the control of the type-3 promoter, allowed us to design a novel strategy to characterize TRs in early diverging Viridiplantae taxa, as well as in ciliates and other Diaphoretickes lineages. Starting with the characterization of the upstream sequence element of the type 3 promoter that is conserved in a number of small nuclear RNAs, and the expected minimum TR template region as search features, we identified candidate TRs in selected Diaphoretickes genomes. Homologous TRs were then used to build covariance models to identify TRs in more distant species. Transcripts of the identified TRs were confirmed by transcriptomic data, RT-PCR and Northern hybridization. A templating role for one of our candidates was validated in Physcomitrium patens. Analysis of secondary structure demonstrated a deep conservation of motifs (pseudoknot and template boundary element) observed in all published TRs. These results elucidate the evolution of the earliest eukaryotic TRs. linking the common origin of TRs across Diaphoretickes, and underlying evolutionary transitions in telomere repeats. [GRAPHICS] .
Links
GX20-01331X, research and development project |
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LM2018140, research and development project |
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90132, large research infrastructures |
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