J 2021

Evolution of plant telomerase RNAs: farther to the past, deeper to the roots

FAJKUS, Petr, Agata Magdalena KILAR, A.D.L. NELSON, M. HOLA, V. PESKA et. al.

Basic information

Original name

Evolution of plant telomerase RNAs: farther to the past, deeper to the roots

Authors

FAJKUS, Petr (203 Czech Republic, belonging to the institution), Agata Magdalena KILAR (616 Poland, belonging to the institution), A.D.L. NELSON, M. HOLA, V. PESKA, Ivana GOFFOVÁ (703 Slovakia, belonging to the institution), Miloslava FOJTOVÁ (203 Czech Republic, belonging to the institution), Dagmar ZACHOVÁ (203 Czech Republic, belonging to the institution), Jana FULNEČKOVÁ (203 Czech Republic) and Jiří FAJKUS (203 Czech Republic, guarantor, belonging to the institution)

Edition

Nucleic acids research, Oxford, Oxford University Press, 2021, 0305-1048

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 19.160

RIV identification code

RIV/00216224:14740/21:00119679

Organization unit

Central European Institute of Technology

UT WoS

000685211300038

Keywords in English

TEMPLATE-BOUNDARY DEFINITION; SECONDARY STRUCTURE; POLYMERASE-III; SNRNA GENES; SEQUENCE; IDENTIFICATION; PROMOTER; PSEUDOKNOT; ELEMENT; ARABIDOPSIS

Tags

International impact, Reviewed
Změněno: 15/10/2024 14:07, Ing. Martina Blahová

Abstract

V originále

The enormous sequence heterogeneity of telomerase RNA (TR) subunits has thus far complicated their characterization in a wider phylogenetic range. Our recent finding that land plant TRs are, similarly to known ciliate TRs, transcribed by RNA polymerase III and under the control of the type-3 promoter, allowed us to design a novel strategy to characterize TRs in early diverging Viridiplantae taxa, as well as in ciliates and other Diaphoretickes lineages. Starting with the characterization of the upstream sequence element of the type 3 promoter that is conserved in a number of small nuclear RNAs, and the expected minimum TR template region as search features, we identified candidate TRs in selected Diaphoretickes genomes. Homologous TRs were then used to build covariance models to identify TRs in more distant species. Transcripts of the identified TRs were confirmed by transcriptomic data, RT-PCR and Northern hybridization. A templating role for one of our candidates was validated in Physcomitrium patens. Analysis of secondary structure demonstrated a deep conservation of motifs (pseudoknot and template boundary element) observed in all published TRs. These results elucidate the evolution of the earliest eukaryotic TRs. linking the common origin of TRs across Diaphoretickes, and underlying evolutionary transitions in telomere repeats. [GRAPHICS] .

Links

GX20-01331X, research and development project
Name: Biogeneze a evoluce telomerázy u rostlin
Investor: Czech Science Foundation
LM2018140, research and development project
Name: e-Infrastruktura CZ (Acronym: e-INFRA CZ)
Investor: Ministry of Education, Youth and Sports of the CR
90132, large research infrastructures
Name: NCMG II