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@article{1838542,
author = {Shan, Wenbo and Kubová, Michaela and Malík Mandáková, Terezie and Lysák, Martin},
article_location = {Hoboken},
article_number = {2},
doi = {http://dx.doi.org/10.1111/tpj.15459},
keywords = {interphase; chromocenter-loop model; Rabl; centromere; telomere; nucleolus; Arabidopsis thaliana; Crucifereae},
language = {eng},
issn = {0960-7412},
journal = {Plant Journal},
title = {Nuclear organization in crucifer genomes: nucleolus-associated telomere clustering is not a universal interphase configuration in Brassicaceae},
url = {https://onlinelibrary.wiley.com/doi/full/10.1111/tpj.15459},
volume = {108},
year = {2021}
}
TY - JOUR
ID - 1838542
AU - Shan, Wenbo - Kubová, Michaela - Malík Mandáková, Terezie - Lysák, Martin
PY - 2021
TI - Nuclear organization in crucifer genomes: nucleolus-associated telomere clustering is not a universal interphase configuration in Brassicaceae
JF - Plant Journal
VL - 108
IS - 2
SP - 528-540
EP - 528-540
PB - Wiley-Blackwell
SN - 09607412
KW - interphase
KW - chromocenter-loop model
KW - Rabl
KW - centromere
KW - telomere
KW - nucleolus
KW - Arabidopsis thaliana
KW - Crucifereae
UR - https://onlinelibrary.wiley.com/doi/full/10.1111/tpj.15459
N2 - Arabidopsis thaliana has become a major plant research model, where interphase nuclear organization exhibits unique features, including nucleolus-associated telomere clustering. The chromocenter (CC)-loop model, or rosette-like configuration, describes intranuclear chromatin organization in Arabidopsis as megabase-long loops anchored in, and emanating from, peripherally positioned CCs, with those containing telomeres associating with the nucleolus. To investigate whether the CC-loop organization is universal across the mustard family (crucifers), the nuclear distributions of centromeres, telomeres and nucleoli were analyzed by fluorescence in situ hybridization in seven diploid species (2n = 10-16) representing major crucifer clades with an up to 26-fold variation in genome size (160-4260 Mb). Nucleolus-associated telomere clustering was confirmed in Arabidopsis (157 Mb) and was newly identified as the major nuclear phenotype in other species with a small genome (215-381 Mb). In large-genome species (2611-4264 Mb), centromeres and telomeres adopted a Rabl-like configuration or dispersed distribution in the nuclear interior; telomeres only rarely associated with the nucleolus. In Arabis cypria (381 Mb) and Bunias orientalis (2611 Mb), tissue-specific patterns deviating from the major nuclear phenotypes were observed in anther and stem tissues, respectively. The rosette-like configuration, including nucleolus-associated telomere clustering in small-genome species from different infrafamiliar clades, suggests that genomic properties rather than phylogenetic position determine the interphase nuclear organization. Our data suggest that nuclear genome size, average chromosome size and degree of longitudinal chromosome compartmentalization affect interphase chromosome organization in crucifer genomes.
ER -
SHAN, Wenbo, Michaela KUBOVÁ, Terezie MALÍK MANDÁKOVÁ and Martin LYSÁK. Nuclear organization in crucifer genomes: nucleolus-associated telomere clustering is not a universal interphase configuration in Brassicaceae. \textit{Plant Journal}. Hoboken: Wiley-Blackwell, 2021, vol.~108, No~2, p.~528-540. ISSN~0960-7412. Available from: https://dx.doi.org/10.1111/tpj.15459.
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