Nuclear organization in crucifer genomes: nucleolus-associated
telomere clustering is not a universal interphase configuration
in Brassicaceae

J 2021

Nuclear organization in crucifer genomes: nucleolus-associated telomere clustering is not a universal interphase configuration in Brassicaceae

SHAN, Wenbo, Michaela KUBOVÁ, Terezie MALÍK MANDÁKOVÁ and Martin LYSÁK

Basic information

Original name

Nuclear organization in crucifer genomes: nucleolus-associated telomere clustering is not a universal interphase configuration in Brassicaceae

Authors

SHAN, Wenbo (156 China, belonging to the institution), Michaela KUBOVÁ (203 Czech Republic, belonging to the institution), Terezie MALÍK MANDÁKOVÁ (203 Czech Republic, belonging to the institution) and Martin LYSÁK (203 Czech Republic, guarantor, belonging to the institution)

Edition

Plant Journal, Hoboken, Wiley-Blackwell, 2021, 0960-7412

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10611 Plant sciences, botany

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 7.091

RIV identification code

RIV/00216224:14740/21:00119689

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1111/tpj.15459

UT WoS

000694977300001

Keywords in English

interphase; chromocenter-loop model; Rabl; centromere; telomere; nucleolus; Arabidopsis thaliana; Crucifereae

Abstract

V originále

Arabidopsis thaliana has become a major plant research model, where interphase nuclear organization exhibits unique features, including nucleolus-associated telomere clustering. The chromocenter (CC)-loop model, or rosette-like configuration, describes intranuclear chromatin organization in Arabidopsis as megabase-long loops anchored in, and emanating from, peripherally positioned CCs, with those containing telomeres associating with the nucleolus. To investigate whether the CC-loop organization is universal across the mustard family (crucifers), the nuclear distributions of centromeres, telomeres and nucleoli were analyzed by fluorescence in situ hybridization in seven diploid species (2n = 10-16) representing major crucifer clades with an up to 26-fold variation in genome size (160-4260 Mb). Nucleolus-associated telomere clustering was confirmed in Arabidopsis (157 Mb) and was newly identified as the major nuclear phenotype in other species with a small genome (215-381 Mb). In large-genome species (2611-4264 Mb), centromeres and telomeres adopted a Rabl-like configuration or dispersed distribution in the nuclear interior; telomeres only rarely associated with the nucleolus. In Arabis cypria (381 Mb) and Bunias orientalis (2611 Mb), tissue-specific patterns deviating from the major nuclear phenotypes were observed in anther and stem tissues, respectively. The rosette-like configuration, including nucleolus-associated telomere clustering in small-genome species from different infrafamiliar clades, suggests that genomic properties rather than phylogenetic position determine the interphase nuclear organization. Our data suggest that nuclear genome size, average chromosome size and degree of longitudinal chromosome compartmentalization affect interphase chromosome organization in crucifer genomes.

Links

GA18-20134S, research and development project

Name: Organizace interfázních chromosomů a časné meiotické párování chromosomů u brukvovitých

Investor: Czech Science Foundation

MUNI/IGA/1352/2020, interní kód MU

Name: Plant meiotic chromosome dynamics in three dimensions

Investor: Masaryk University

90129, large research infrastructures

Name: Czech-BioImaging II

Citovat

SHAN, Wenbo, Michaela KUBOVÁ, Terezie MALÍK MANDÁKOVÁ and Martin LYSÁK. Nuclear organization in crucifer genomes: nucleolus-associated telomere clustering is not a universal interphase configuration in Brassicaceae. Plant Journal. Hoboken: Wiley-Blackwell, 2021, vol. 108, No 2, p. 528-540. ISSN 0960-7412. Available from: https://dx.doi.org/10.1111/tpj.15459.

@article{1838542,
   author = {Shan, Wenbo and Kubová, Michaela and Malík Mandáková, Terezie and Lysák, Martin},
   article_location = {Hoboken},
   article_number = {2},
   doi = {http://dx.doi.org/10.1111/tpj.15459},
   keywords = {interphase; chromocenter-loop model; Rabl; centromere; telomere; nucleolus; Arabidopsis thaliana; Crucifereae},
   language = {eng},
   issn = {0960-7412},
   journal = {Plant Journal},
   title = {Nuclear organization in crucifer genomes: nucleolus-associated telomere clustering is not a universal interphase configuration in Brassicaceae},
   url = {https://onlinelibrary.wiley.com/doi/full/10.1111/tpj.15459},
   volume = {108},
   year = {2021}
}

TY  - JOUR
ID  - 1838542
AU  - Shan, Wenbo - Kubová, Michaela - Malík Mandáková, Terezie - Lysák, Martin
PY  - 2021
TI  - Nuclear organization in crucifer genomes: nucleolus-associated telomere clustering is not a universal interphase configuration in Brassicaceae
JF  - Plant Journal
VL  - 108
IS  - 2
SP  - 528-540
EP  - 528-540
PB  - Wiley-Blackwell
SN  - 09607412
KW  - interphase
KW  - chromocenter-loop model
KW  - Rabl
KW  - centromere
KW  - telomere
KW  - nucleolus
KW  - Arabidopsis thaliana
KW  - Crucifereae
UR  - https://onlinelibrary.wiley.com/doi/full/10.1111/tpj.15459
N2  - Arabidopsis thaliana has become a major plant research model, where interphase nuclear organization exhibits unique features, including nucleolus-associated telomere clustering. The chromocenter (CC)-loop model, or rosette-like configuration, describes intranuclear chromatin organization in Arabidopsis as megabase-long loops anchored in, and emanating from, peripherally positioned CCs, with those containing telomeres associating with the nucleolus. To investigate whether the CC-loop organization is universal across the mustard family (crucifers), the nuclear distributions of centromeres, telomeres and nucleoli were analyzed by fluorescence in situ hybridization in seven diploid species (2n = 10-16) representing major crucifer clades with an up to 26-fold variation in genome size (160-4260 Mb). Nucleolus-associated telomere clustering was confirmed in Arabidopsis (157 Mb) and was newly identified as the major nuclear phenotype in other species with a small genome (215-381 Mb). In large-genome species (2611-4264 Mb), centromeres and telomeres adopted a Rabl-like configuration or dispersed distribution in the nuclear interior; telomeres only rarely associated with the nucleolus. In Arabis cypria (381 Mb) and Bunias orientalis (2611 Mb), tissue-specific patterns deviating from the major nuclear phenotypes were observed in anther and stem tissues, respectively. The rosette-like configuration, including nucleolus-associated telomere clustering in small-genome species from different infrafamiliar clades, suggests that genomic properties rather than phylogenetic position determine the interphase nuclear organization. Our data suggest that nuclear genome size, average chromosome size and degree of longitudinal chromosome compartmentalization affect interphase chromosome organization in crucifer genomes.
ER  -

SHAN, Wenbo, Michaela KUBOVÁ, Terezie MALÍK MANDÁKOVÁ and Martin LYSÁK. Nuclear organization in crucifer genomes: nucleolus-associated telomere clustering is not a universal interphase configuration in Brassicaceae. \textit{Plant Journal}. Hoboken: Wiley-Blackwell, 2021, vol.~108, No~2, p.~528-540. ISSN~0960-7412. Available from: https://dx.doi.org/10.1111/tpj.15459.

Detailed Information on Publication Record