J 2021

The oligomeric states of elicitins affect the hypersensitive response and resistance in tobacco

SOLANSKÝ, Martin, Kamil MIKULÁŠEK, Martina ZAPLETALOVÁ, Marek PETŘIVALSKÝ, Annick CHILTZ et. al.

Basic information

Original name

The oligomeric states of elicitins affect the hypersensitive response and resistance in tobacco

Authors

SOLANSKÝ, Martin (203 Czech Republic, belonging to the institution), Kamil MIKULÁŠEK (203 Czech Republic, belonging to the institution), Martina ZAPLETALOVÁ (203 Czech Republic, belonging to the institution), Marek PETŘIVALSKÝ, Annick CHILTZ, Zbyněk ZDRÁHAL (203 Czech Republic, belonging to the institution), Nathalie LEBORGNE-CASTEL and Jan LOCHMAN (203 Czech Republic, guarantor, belonging to the institution)

Edition

Journal of Experimental Botany, Oxford, Oxford University Press, 2021, 0022-0957

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10611 Plant sciences, botany

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 7.298

RIV identification code

RIV/00216224:14310/21:00124352

Organization unit

Faculty of Science

DOI

UT WoS

000642310500030

Keywords in English

Cell wall; elicitin beta-CRY; ELICITIN RESPONSE; hypersensitive response; Nicotiana tabacum; oligomeric structure; Phytophthora; resistance; signalling; SUPPRESSOR OF BIR1-1 (SOBIR1); tobacco

Tags

Tags

International impact, Reviewed
Změněno: 15/10/2024 12:47, Ing. Marie Švancarová

Abstract

V originále

Successful plant defence against microbial pathogens is based on early recognition and fast activation of inducible responses. Key mechanisms include detection of microbe-associated molecular patterns by membrane-localized pattern recognition receptors that induce a basal resistance response. A well-described model of such responses to pathogens involves the interactions between Solanaceae plants and proteinaceous elicitors secreted by oomycetes, called elicitins. It has been hypothesized that the formation of oligomeric structures by elicitins could be involved in their recognition and activation of defensive transduction cascades. In this study, we tested this hypothesis using several approaches, and we observed differences in tobacco plant responses induced by the elicitin beta-cryptogein (beta-CRY) and its homodimer, beta-CRYDIM. We also found that the C-terminal domain of elicitins of other ELI (true-elicitin) clades plays a significant role in stabilization of their oligomeric structure and restraint in the cell wall. In addition, covalently cross-linking beta-CRYDIM impaired the formation of signalling complexes, thereby reducing its capacity to elicit the hypersensitive response and resistance in the host plant, with no significant changes in pathogenesis-related protein expression. By revealing the details of the effects of beta-CRY dimerization on recognition and defence responses in tobacco, our results shed light on the poorly understood role of elicitins' oligomeric structures in the interactions between oomycetes and plants.

Links

LQ1601, research and development project
Name: CEITEC 2020 (Acronym: CEITEC2020)
Investor: Ministry of Education, Youth and Sports of the CR
90127, large research infrastructures
Name: CIISB II