KREMSEROVÁ, Silvie, Anna KOCURKOVÁ, Michaela CHORVÁTOVÁ, Anna KLINKE and Lukáš KUBALA. Myeloperoxidase Deficiency Alters the Process of the Regulated Cell Death of Polymorphonuclear Neutrophils. Frontiers in Immunology. Frontiers Media SA, 2022, vol. 13, February, p. 707085-707100. ISSN 1664-3224. Available from: https://dx.doi.org/10.3389/fimmu.2022.707085.
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Basic information
Original name Myeloperoxidase Deficiency Alters the Process of the Regulated Cell Death of Polymorphonuclear Neutrophils
Authors KREMSEROVÁ, Silvie, Anna KOCURKOVÁ (203 Czech Republic, belonging to the institution), Michaela CHORVÁTOVÁ (703 Slovakia, belonging to the institution), Anna KLINKE and Lukáš KUBALA (203 Czech Republic, guarantor, belonging to the institution).
Edition Frontiers in Immunology, Frontiers Media SA, 2022, 1664-3224.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 30102 Immunology
Country of publisher Switzerland
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 7.300
RIV identification code RIV/00216224:14310/22:00125502
Organization unit Faculty of Science
Doi http://dx.doi.org/10.3389/fimmu.2022.707085
UT WoS 000760482000001
Keywords in English myeloperoxidase; inflammation; neutrophils; apoptosis; cell death; annexin V
Tags rivok
Tags International impact, Reviewed
Changed by Changed by: Mgr. Marie Šípková, DiS., učo 437722. Changed: 14/3/2022 10:54.
Abstract
Polymorphonuclear neutrophils (PMNs) play a key role in host defense. However, their massive accumulation at the site of inflammation can delay regenerative healing processes and can initiate pathological inflammatory processes. Thus, the efficient clearance of PMNs mediated by the induction of regulated cell death is a key process preventing the development of these pathological conditions. Myeloperoxidase (MPO), a highly abundant enzyme in PMN granules, primarily connected with PMN defense machinery, is suggested to play a role in PMN-regulated cell death. However, the contribution of MPO to the mechanisms of PMN cell death remains incompletely characterized. Herein, the process of the cell death of mouse PMNs induced by three different stimuli - phorbol 12-myristate 13-acetate (PMA), opsonized streptococcus (OST), and N-formyl-met-leu-phe (fMLP) - was investigated. MPO-deficient PMNs revealed a significantly decreased rate of cell death characterized by phosphatidylserine surface exposure and cell membrane permeabilization. An inhibitor of MPO activity, 4-aminobenzoic acid hydrazide, did not exhibit a significant effect on PMA-induced cell death compared to MPO deficiency. Interestingly, only the limited activation of markers related to apoptotic cell death was observed (e.g. caspase 8 activation, Bax expression) and they mostly did not correspond to phosphatidylserine surface exposure. Furthermore, a marker characterizing autophagy, cleavage of LC3 protein, as well as histone H3 citrullination and its surface expression was observed. Collectively, the data show the ability of MPO to modulate the life span of PMNs primarily through the potentiation of cell membrane permeabilization and phosphatidylserine surface exposure.
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