A novel temperature-controlled open source microcontroller
based sampler for collection of exhaled breath condensate in
point-of-care diagnostics

J 2022

A novel temperature-controlled open source microcontroller based sampler for collection of exhaled breath condensate in point-of-care diagnostics

DOSEDĚLOVÁ, Věra, František FORET, Martina DOUBKOVÁ, Kristián BRAT, Petr KUBÁŇ et. al.

Basic information

Original name

A novel temperature-controlled open source microcontroller based sampler for collection of exhaled breath condensate in point-of-care diagnostics

Authors

DOSEDĚLOVÁ, Věra (203 Czech Republic, belonging to the institution), František FORET, Martina DOUBKOVÁ (203 Czech Republic, belonging to the institution), Kristián BRAT (703 Slovakia, belonging to the institution) and Petr KUBÁŇ (203 Czech Republic, guarantor)

Edition

Talanta, Elsevier, 2022, 0039-9140

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10406 Analytical chemistry

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 6.100

RIV identification code

RIV/00216224:14310/22:00125512

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1016/j.talanta.2021.122984

UT WoS

000711385900002

Keywords in English

Exhaled breath condensate; Arduino; Collection device; Temperature control; Ion analysis; Capillary electrophoresis

Abstract

V originále

Exhaled breath condensate (EBC) is an attractive, non-invasive sample for clinical diagnostics. During EBC collection, its composition is influenced by the collection temperature, a factor that is often not thoroughly monitored and controlled. In this study, we assembled a novel, simple, portable, and inexpensive device for EBC collection, able to maintain a stable temperature at any value between -7 degrees C and +12 degrees C. The temperature was controlled using a microcontroller and a thermoelectric cooler that was employed to cool the aluminum block holding the glass tube or the polypropylene syringe. The performance of the novel sampler was compared with the passively cooled RTubeTM and a simple EBC sampler, in which the temperature was steadily increasing during sampling. The developed sampler was able to maintain a stable temperature within +/- 1 degrees C. To investigate the influence of different sampling temperatures (i.e., +12,-7, -80 degrees C) on the analyte content in EBC, inorganic ions and organic acids were analyzed by capillary electrophoresis with a capacitively coupled contactless conductivity detector. It was shown that the concentration of metabolites decreased significantly with decreasing temperature. The portability and the ability to keep a stable temperature during EBC sampling makes the developed sampler suitable for point-of-care diagnostics.

Citovat

DOSEDĚLOVÁ, Věra, František FORET, Martina DOUBKOVÁ, Kristián BRAT and Petr KUBÁŇ. A novel temperature-controlled open source microcontroller based sampler for collection of exhaled breath condensate in point-of-care diagnostics. Talanta. Elsevier, 2022, vol. 237, January, p. 1-8. ISSN 0039-9140. Available from: https://dx.doi.org/10.1016/j.talanta.2021.122984.

@article{1840657,
   author = {Dosedělová, Věra and Foret, František and Doubková, Martina and Brat, Kristián and Kubáň, Petr},
   article_number = {January},
   doi = {http://dx.doi.org/10.1016/j.talanta.2021.122984},
   keywords = {Exhaled breath condensate; Arduino; Collection device; Temperature control; Ion analysis; Capillary electrophoresis},
   language = {eng},
   issn = {0039-9140},
   journal = {Talanta},
   title = {A novel temperature-controlled open source microcontroller based sampler for collection of exhaled breath condensate in point-of-care diagnostics},
   url = {https://www.sciencedirect.com/science/article/pii/S0039914021009061},
   volume = {237},
   year = {2022}
}

TY  - JOUR
ID  - 1840657
AU  - Dosedělová, Věra - Foret, František - Doubková, Martina - Brat, Kristián - Kubáň, Petr
PY  - 2022
TI  - A novel temperature-controlled open source microcontroller based sampler for collection of exhaled breath condensate in point-of-care diagnostics
JF  - Talanta
VL  - 237
IS  - January
SP  - 1-8
EP  - 1-8
PB  - Elsevier
SN  - 00399140
KW  - Exhaled breath condensate
KW  - Arduino
KW  - Collection device
KW  - Temperature control
KW  - Ion analysis
KW  - Capillary electrophoresis
UR  - https://www.sciencedirect.com/science/article/pii/S0039914021009061
N2  - Exhaled breath condensate (EBC) is an attractive, non-invasive sample for clinical diagnostics. During EBC collection, its composition is influenced by the collection temperature, a factor that is often not thoroughly monitored and controlled. In this study, we assembled a novel, simple, portable, and inexpensive device for EBC collection, able to maintain a stable temperature at any value between -7 degrees C and +12 degrees C. The temperature was controlled using a microcontroller and a thermoelectric cooler that was employed to cool the aluminum block holding the glass tube or the polypropylene syringe. The performance of the novel sampler was compared with the passively cooled RTubeTM and a simple EBC sampler, in which the temperature was steadily increasing during sampling. The developed sampler was able to maintain a stable temperature within +/- 1 degrees C. To investigate the influence of different sampling temperatures (i.e., +12,-7, -80 degrees C) on the analyte content in EBC, inorganic ions and organic acids were analyzed by capillary electrophoresis with a capacitively coupled contactless conductivity detector. It was shown that the concentration of metabolites decreased significantly with decreasing temperature. The portability and the ability to keep a stable temperature during EBC sampling makes the developed sampler suitable for point-of-care diagnostics.
ER  -

DOSEDĚLOVÁ, Věra, František FORET, Martina DOUBKOVÁ, Kristián BRAT and Petr KUBÁŇ. A novel temperature-controlled open source microcontroller based sampler for collection of exhaled breath condensate in point-of-care diagnostics. \textit{Talanta}. Elsevier, 2022, vol.~237, January, p.~1-8. ISSN~0039-9140. Available from: https://dx.doi.org/10.1016/j.talanta.2021.122984.

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