Elevated epidermal growth factor receptor levels in Barrett’s
esophagus

a 2021

Elevated epidermal growth factor receptor levels in Barrett’s esophagus

DEISSOVÁ, Tereza, Lydie IZAKOVIČOVÁ HOLLÁ, Zuzana ZÁKOSTELSKÁ JIRÁSKOVÁ, Jiří DOLINA, Radek KROUPA et. al.

Basic information

Original name

Elevated epidermal growth factor receptor levels in Barrett’s esophagus

Authors

DEISSOVÁ, Tereza (203 Czech Republic, belonging to the institution), Lydie IZAKOVIČOVÁ HOLLÁ (203 Czech Republic, belonging to the institution), Zuzana ZÁKOSTELSKÁ JIRÁSKOVÁ (203 Czech Republic), Jiří DOLINA (203 Czech Republic, belonging to the institution), Radek KROUPA (203 Czech Republic, belonging to the institution), Lumír KUNOVSKÝ (203 Czech Republic, belonging to the institution), Zdeněk PAVLOVSKÝ (203 Czech Republic, belonging to the institution), Břetislav LIPOVÝ (203 Czech Republic, belonging to the institution), Zdeněk DANĚK (203 Czech Republic, belonging to the institution), Zdeněk KALA (203 Czech Republic, belonging to the institution), Ondřej SLABÝ (203 Czech Republic, belonging to the institution), Ondřej URBAN (203 Czech Republic), Vít NAVRÁTIL (203 Czech Republic), Tomáš HARUŠTIAK (203 Czech Republic), Robert LISCHKE (203 Czech Republic) and Petra BOŘILOVÁ LINHARTOVÁ (203 Czech Republic, guarantor, belonging to the institution)

Edition

UEG Week Virtual 2021, United European Gastroenterology Journal 2021; 9 (Supplement 1), 2021

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

30300 3.3 Health sciences

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14110/21:00120236

Organization unit

Faculty of Medicine

Keywords in English

epidermal growth factor; gastroesophageal reflux; polymorphism; case-control study;

Abstract

V originále

Introduction: Gastroesophageal reflux disease (GERD) can manifest through various symptoms including mucosal damage, in which case we speak of reflux esophagitis (RE), Barrett’s esophagus (BE) or esophageal adenocarcinoma (EAC). The epidermal growth factor (EGF) and its receptor (EGFR) is pivotal in the maintenance of the integrity of the esophageal mucosa and repair of injured tissues. On the other hand, upregulation of the EGF signaling pathway has been shown to lead to neoplastic transformation; in addition, EGFR expression was also associated with GERD progression.1-8 Aims & methods: The aim of our study was to compare the expression of EGF and EGFR in the esophageal tissues with vs. without pathological changes in GERD patients. The EGF and EGFR mRNA expressions were analyzed by reverse transcription qPCR in 50 fresh esophageal tissue biopsies with/without pathological changes taken from 25 patients with GERD (RE [N=10], BE [N=8], EAC [N=7]). In addition, the EGFR protein expression was examined in 50 corresponding formalin-fixed paraffin esophageal tissues using immunohistochemistry. The mRNA and protein expressions were statistically evaluated using the Wilcoxon signed ranked test in Statistica v13.2. Results: No differences in the EGF or EGFR mRNA expressions in the esophageal tissues with and without pathological changes were detected in neither of the patient groups (p>0.05). However, the EGFR protein levels in BE biopsies were higher than in corresponding samples without metaplastic changes.(p=0.034). Conclusion: Although the study is limited by the small number of patients, our results suggest that EGFR may possibly serve as a biomarker of BE development. This is the first study comparing EGF and its receptor expressions in multiple sites of the esophagus in the same patient with GERD.

Links

NU20-03-00126, research and development project

Name: Hostitelský mikrobiom ve vztahu k rozvoji Barrettova jícnu a adenokarcinomu jícnu

Investor: Ministry of Health of the CR, Host microbiome in relation to Barrett ́s esophagus and esophageal adenocarcinoma development, Subprogram 1 - standard

Citovat

DEISSOVÁ, Tereza, Lydie IZAKOVIČOVÁ HOLLÁ, Zuzana ZÁKOSTELSKÁ JIRÁSKOVÁ, Jiří DOLINA, Radek KROUPA, Lumír KUNOVSKÝ, Zdeněk PAVLOVSKÝ, Břetislav LIPOVÝ, Zdeněk DANĚK, Zdeněk KALA, Ondřej SLABÝ, Ondřej URBAN, Vít NAVRÁTIL, Tomáš HARUŠTIAK, Robert LISCHKE and Petra BOŘILOVÁ LINHARTOVÁ. Elevated epidermal growth factor receptor levels in Barrett’s esophagus. In UEG Week Virtual 2021, United European Gastroenterology Journal 2021; 9 (Supplement 1). 2021.

@proceedings{1840789,
   author = {Deissová, Tereza and Izakovičová Hollá, Lydie and Zákostelská Jirásková, Zuzana and Dolina, Jiří and Kroupa, Radek and Kunovský, Lumír and Pavlovský, Zdeněk and Lipový, Břetislav and Daněk, Zdeněk and Kala, Zdeněk and Slabý, Ondřej and Urban, Ondřej and Navrátil, Vít and Haruštiak, Tomáš and Lischke, Robert and Bořilová Linhartová, Petra},
   booktitle = {UEG Week Virtual 2021, United European Gastroenterology Journal 2021; 9 (Supplement 1)},
   keywords = {epidermal growth factor; gastroesophageal reflux; polymorphism; case-control study;},
   language = {eng},
   title = {Elevated epidermal growth factor receptor levels in Barrett’s esophagus},
   year = {2021}
}

TY  - CONF
ID  - 1840789
AU  - Deissová, Tereza - Izakovičová Hollá, Lydie - Zákostelská Jirásková, Zuzana - Dolina, Jiří - Kroupa, Radek - Kunovský, Lumír - Pavlovský, Zdeněk - Lipový, Břetislav - Daněk, Zdeněk - Kala, Zdeněk - Slabý, Ondřej - Urban, Ondřej - Navrátil, Vít - Haruštiak, Tomáš - Lischke, Robert - Bořilová Linhartová, Petra
PY  - 2021
TI  - Elevated epidermal growth factor receptor levels in Barrett’s esophagus
KW  - epidermal growth factor
KW  - gastroesophageal reflux
KW  - polymorphism
KW  - case-control study;
N2  - Introduction: Gastroesophageal reflux disease (GERD) can manifest through various symptoms including mucosal damage, in which case we speak of reflux esophagitis (RE), Barrett’s esophagus (BE) or esophageal adenocarcinoma (EAC). The epidermal growth factor (EGF) and its receptor (EGFR) is pivotal in the maintenance of the integrity of the esophageal mucosa and repair of injured tissues. On the other hand, upregulation of the EGF signaling pathway has been shown to lead to neoplastic transformation; in addition, EGFR expression was also associated with GERD progression.1-8 Aims & methods: The aim of our study was to compare the expression of EGF and EGFR in the esophageal tissues with vs. without pathological changes in GERD patients. The EGF and EGFR mRNA expressions were analyzed by reverse transcription qPCR in 50 fresh esophageal tissue biopsies with/without pathological changes taken from 25 patients with GERD (RE [N=10], BE [N=8], EAC [N=7]). In addition, the EGFR protein expression was examined in 50 corresponding formalin-fixed paraffin esophageal tissues using immunohistochemistry. The mRNA and protein expressions were statistically evaluated using the Wilcoxon signed ranked test in Statistica v13.2. Results: No differences in the EGF or EGFR mRNA expressions in the esophageal tissues with and without pathological changes were detected in neither of the patient groups (p>0.05). However, the EGFR protein levels in BE biopsies were higher than in corresponding samples without metaplastic changes.(p=0.034). Conclusion: Although the study is limited by the small number of patients, our results suggest that EGFR may possibly serve as a biomarker of BE development. This is the first study comparing EGF and its receptor expressions in multiple sites of the esophagus in the same patient with GERD.
ER  -

DEISSOVÁ, Tereza, Lydie IZAKOVIČOVÁ HOLLÁ, Zuzana ZÁKOSTELSKÁ JIRÁSKOVÁ, Jiří DOLINA, Radek KROUPA, Lumír KUNOVSKÝ, Zdeněk PAVLOVSKÝ, Břetislav LIPOVÝ, Zdeněk DANĚK, Zdeněk KALA, Ondřej SLABÝ, Ondřej URBAN, Vít NAVRÁTIL, Tomáš HARUŠTIAK, Robert LISCHKE and Petra BOŘILOVÁ LINHARTOVÁ. Elevated epidermal growth factor receptor levels in Barrett’s esophagus. In \textit{UEG Week Virtual 2021, United European Gastroenterology Journal 2021; 9 (Supplement 1)}. 2021.

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