| MANDAL, Govinda, Lukáš MORÁŇ, Lukáš PEČINKA, Petr VAŇHARA and Josef HAVEL. Matrix enrichment by black phosphorus improves ionization and reproducibility of mass spectrometry of intact cells, peptides, and amino acids. Nature Scientific Reports. London: Nature Research, 2022, vol. 12, No 1, p. 1-10. ISSN 2045-2322. Available from: https://dx.doi.org/10.1038/s41598-022-05197-9. |
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@article{1840897,
author = {Mandal, Govinda and Moráň, Lukáš and Pečinka, Lukáš and Vaňhara, Petr and Havel, Josef},
article_location = {London},
article_number = {1},
doi = {http://dx.doi.org/10.1038/s41598-022-05197-9},
keywords = {stem cells; laser desorption/ionization mass spectrometry (MALDI-TOF MS)},
language = {eng},
issn = {2045-2322},
journal = {Nature Scientific Reports},
title = {Matrix enrichment by black phosphorus improves ionization and reproducibility of mass spectrometry of intact cells, peptides, and amino acids},
url = {https://www.nature.com/articles/s41598-022-05197-9},
volume = {12},
year = {2022}
}
TY - JOUR
ID - 1840897
AU - Mandal, Govinda - Moráň, Lukáš - Pečinka, Lukáš - Vaňhara, Petr - Havel, Josef
PY - 2022
TI - Matrix enrichment by black phosphorus improves ionization and reproducibility of mass spectrometry of intact cells, peptides, and amino acids
JF - Nature Scientific Reports
VL - 12
IS - 1
SP - 1-10
EP - 1-10
PB - Nature Research
SN - 20452322
KW - stem cells
KW - laser desorption/ionization mass spectrometry (MALDI-TOF MS)
UR - https://www.nature.com/articles/s41598-022-05197-9
N2 - Intact (whole) cell matrix-assisted laser desorption/ionization mass spectrometry (MALDI-TOF MS) is an established method for biotyping in clinical microbiology as well as for revealing phenotypic shifts in cultured eukaryotic cells. Intact cell MALDI-TOF MS has recently been introduced as a quality control tool for long-term cultures of pluripotent stem cells. Despite the potential this method holds for revealing minute changes in cells, there is still a need for improving the ionization efficiency or peak reproducibility. Here we report for the first time that supplementation by fine particles of black phosphorus to the standard MALDI matrices, such as sinapinic and alpha-cyano-4-hydroxycinnamic acids enhance intensities of mass spectra of particular amino acids and peptides, presumably by interactions with aromatic groups within the molecules. In addition, the particles of black phosphorus induce the formation of small and regularly dispersed crystals of sinapinic acid and alpha-cyano-4-hydroxycinnamic acid with the analyte on a steel MALDI target plate. Patterns of mass spectra recorded from intact cells using black phosphorus-enriched matrix were more reproducible and contained peaks of higher intensities when compared to matrix without black phosphorus supplementation. In summary, enrichment of common organic matrices by black phosphorus can improve discrimination data analysis by enhancing peak intensity and reproducibility of mass spectra acquired from intact cells.
ER -
MANDAL, Govinda, Lukáš MORÁŇ, Lukáš PEČINKA, Petr VAŇHARA and Josef HAVEL. Matrix enrichment by black phosphorus improves ionization and reproducibility of mass spectrometry of intact cells, peptides, and amino acids. \textit{Nature Scientific Reports}. London: Nature Research, 2022, vol.~12, No~1, p.~1-10. ISSN~2045-2322. Available from: https://dx.doi.org/10.1038/s41598-022-05197-9.
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