| KABELKA, Ivo, Vasil GEORGIEV, Lisa MARX, Peter PAJTINKA, Karl LOHNER, Georg PABST, Rumiana DIMOVA and Robert VÁCHA. Magainin 2 and PGLa in Bacterial Membrane Mimics III: Membrane Fusion and Disruption. Biophysical Journal. New York, USA: Cell Press, 2022, vol. 121, No 5, p. 852-861. ISSN 0006-3495. Available from: https://dx.doi.org/10.1016/j.bpj.2021.12.035. |
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@article{1841097,
author = {Kabelka, Ivo and Georgiev, Vasil and Marx, Lisa and Pajtinka, Peter and Lohner, Karl and Pabst, Georg and Dimova, Rumiana and Vácha, Robert},
article_location = {New York, USA},
article_number = {5},
doi = {http://dx.doi.org/10.1016/j.bpj.2021.12.035},
keywords = {Cell Membrane; Cryoelectron Microscopy; Lipid Bilayers; Magainins; Membrane Fusion},
language = {eng},
issn = {0006-3495},
journal = {Biophysical Journal},
title = {Magainin 2 and PGLa in Bacterial Membrane Mimics III: Membrane Fusion and Disruption.},
url = {https://www.sciencedirect.com/science/article/pii/S000634952103959X?via%3Dihub},
volume = {121},
year = {2022}
}
TY - JOUR
ID - 1841097
AU - Kabelka, Ivo - Georgiev, Vasil - Marx, Lisa - Pajtinka, Peter - Lohner, Karl - Pabst, Georg - Dimova, Rumiana - Vácha, Robert
PY - 2022
TI - Magainin 2 and PGLa in Bacterial Membrane Mimics III: Membrane Fusion and Disruption.
JF - Biophysical Journal
VL - 121
IS - 5
SP - 852-861
EP - 852-861
PB - Cell Press
SN - 00063495
KW - Cell Membrane
KW - Cryoelectron Microscopy
KW - Lipid Bilayers
KW - Magainins
KW - Membrane Fusion
UR - https://www.sciencedirect.com/science/article/pii/S000634952103959X?via%3Dihub
N2 - We previously speculated that the synergistically enhanced antimicrobial activity of Magainin 2 and PGLa is related to membrane adhesion, fusion, and further membrane remodeling. Here we combined computer simulations with time-resolved in vitro fluorescence microscopy, cryoelectron microscopy, and small-angle X-ray scattering to interrogate such morphological and topological changes of vesicles at nanoscopic and microscopic length scales in real time. Coarse grained simulations revealed formation of an elongated and bent fusion zone between vesicles in the presence of equimolar peptide mixtures. Vesicle adhesion and fusion were observed to occur within a few seconds by cryoelectron microscopy and corroborated by small-angle X-ray scattering measurements. The latter experiments indicated continued and time-extended structural remodeling for individual peptides or chemically linked peptide heterodimers but with different kinetics. Fluorescence microscopy further captured peptide-dependent adhesion, fusion, and occasional bursting of giant unilamellar vesicles a few seconds after peptide addition. The synergistic interactions between the peptides shorten the time response of vesicles and enhance membrane fusogenic and disruption properties of the equimolar mixture compared with the individual peptides.
ER -
KABELKA, Ivo, Vasil GEORGIEV, Lisa MARX, Peter PAJTINKA, Karl LOHNER, Georg PABST, Rumiana DIMOVA and Robert VÁCHA. Magainin 2 and PGLa in Bacterial Membrane Mimics III: Membrane Fusion and Disruption. \textit{Biophysical Journal}. New York, USA: Cell Press, 2022, vol.~121, No~5, p.~852-861. ISSN~0006-3495. Available from: https://dx.doi.org/10.1016/j.bpj.2021.12.035.
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