Magainin 2 and PGLa in Bacterial Membrane Mimics III: Membrane
Fusion and Disruption.

J 2022

Magainin 2 and PGLa in Bacterial Membrane Mimics III: Membrane Fusion and Disruption.

KABELKA, Ivo, Vasil GEORGIEV, Lisa MARX, Peter PAJTINKA, Karl LOHNER et. al.

Basic information

Original name

Magainin 2 and PGLa in Bacterial Membrane Mimics III: Membrane Fusion and Disruption.

Authors

KABELKA, Ivo (203 Czech Republic, belonging to the institution), Vasil GEORGIEV, Lisa MARX, Peter PAJTINKA (703 Slovakia, belonging to the institution), Karl LOHNER, Georg PABST, Rumiana DIMOVA and Robert VÁCHA (203 Czech Republic, guarantor, belonging to the institution)

Edition

Biophysical Journal, New York, USA, Cell Press, 2022, 0006-3495

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10610 Biophysics

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.400

RIV identification code

RIV/00216224:14740/22:00125525

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1016/j.bpj.2021.12.035

UT WoS

000765012800016

Keywords in English

Cell Membrane; Cryoelectron Microscopy; Lipid Bilayers; Magainins; Membrane Fusion

Abstract

V originále

We previously speculated that the synergistically enhanced antimicrobial activity of Magainin 2 and PGLa is related to membrane adhesion, fusion, and further membrane remodeling. Here we combined computer simulations with time-resolved in vitro fluorescence microscopy, cryoelectron microscopy, and small-angle X-ray scattering to interrogate such morphological and topological changes of vesicles at nanoscopic and microscopic length scales in real time. Coarse grained simulations revealed formation of an elongated and bent fusion zone between vesicles in the presence of equimolar peptide mixtures. Vesicle adhesion and fusion were observed to occur within a few seconds by cryoelectron microscopy and corroborated by small-angle X-ray scattering measurements. The latter experiments indicated continued and time-extended structural remodeling for individual peptides or chemically linked peptide heterodimers but with different kinetics. Fluorescence microscopy further captured peptide-dependent adhesion, fusion, and occasional bursting of giant unilamellar vesicles a few seconds after peptide addition. The synergistic interactions between the peptides shorten the time response of vesicles and enhance membrane fusogenic and disruption properties of the equimolar mixture compared with the individual peptides.

Links

GA20-20152S, research and development project

Name: Proteinová přitažlivost a selektivita pro buněčné membrány

Investor: Czech Science Foundation

LL2007, research and development project

Name: Peptidoví zabijáci bakterií (Acronym: PeptideKillers)

Investor: Ministry of Education, Youth and Sports of the CR

LM2015085, research and development project

Name: CERIT Scientific Cloud (Acronym: CERIT-SC)

Investor: Ministry of Education, Youth and Sports of the CR, CERIT Scientific Cloud

90127, large research infrastructures

Name: CIISB II

Citovat

KABELKA, Ivo, Vasil GEORGIEV, Lisa MARX, Peter PAJTINKA, Karl LOHNER, Georg PABST, Rumiana DIMOVA and Robert VÁCHA. Magainin 2 and PGLa in Bacterial Membrane Mimics III: Membrane Fusion and Disruption. Biophysical Journal. New York, USA: Cell Press, 2022, vol. 121, No 5, p. 852-861. ISSN 0006-3495. Available from: https://dx.doi.org/10.1016/j.bpj.2021.12.035.

@article{1841097,
   author = {Kabelka, Ivo and Georgiev, Vasil and Marx, Lisa and Pajtinka, Peter and Lohner, Karl and Pabst, Georg and Dimova, Rumiana and Vácha, Robert},
   article_location = {New York, USA},
   article_number = {5},
   doi = {http://dx.doi.org/10.1016/j.bpj.2021.12.035},
   keywords = {Cell Membrane; Cryoelectron Microscopy; Lipid Bilayers; Magainins; Membrane Fusion},
   language = {eng},
   issn = {0006-3495},
   journal = {Biophysical Journal},
   title = {Magainin 2 and PGLa in Bacterial Membrane Mimics III: Membrane Fusion and Disruption.},
   url = {https://www.sciencedirect.com/science/article/pii/S000634952103959X?via%3Dihub},
   volume = {121},
   year = {2022}
}

TY  - JOUR
ID  - 1841097
AU  - Kabelka, Ivo - Georgiev, Vasil - Marx, Lisa - Pajtinka, Peter - Lohner, Karl - Pabst, Georg - Dimova, Rumiana - Vácha, Robert
PY  - 2022
TI  - Magainin 2 and PGLa in Bacterial Membrane Mimics III: Membrane Fusion and Disruption.
JF  - Biophysical Journal
VL  - 121
IS  - 5
SP  - 852-861
EP  - 852-861
PB  - Cell Press
SN  - 00063495
KW  - Cell Membrane
KW  - Cryoelectron Microscopy
KW  - Lipid Bilayers
KW  - Magainins
KW  - Membrane Fusion
UR  - https://www.sciencedirect.com/science/article/pii/S000634952103959X?via%3Dihub
N2  - We previously speculated that the synergistically enhanced antimicrobial activity of Magainin 2 and PGLa is related to membrane adhesion, fusion, and further membrane remodeling. Here we combined computer simulations with time-resolved in vitro fluorescence microscopy, cryoelectron microscopy, and small-angle X-ray scattering to interrogate such morphological and topological changes of vesicles at nanoscopic and microscopic length scales in real time. Coarse grained simulations revealed formation of an elongated and bent fusion zone between vesicles in the presence of equimolar peptide mixtures. Vesicle adhesion and fusion were observed to occur within a few seconds by cryoelectron microscopy and corroborated by small-angle X-ray scattering measurements. The latter experiments indicated continued and time-extended structural remodeling for individual peptides or chemically linked peptide heterodimers but with different kinetics. Fluorescence microscopy further captured peptide-dependent adhesion, fusion, and occasional bursting of giant unilamellar vesicles a few seconds after peptide addition. The synergistic interactions between the peptides shorten the time response of vesicles and enhance membrane fusogenic and disruption properties of the equimolar mixture compared with the individual peptides.
ER  -

KABELKA, Ivo, Vasil GEORGIEV, Lisa MARX, Peter PAJTINKA, Karl LOHNER, Georg PABST, Rumiana DIMOVA and Robert VÁCHA. Magainin 2 and PGLa in Bacterial Membrane Mimics III: Membrane Fusion and Disruption. \textit{Biophysical Journal}. New York, USA: Cell Press, 2022, vol.~121, No~5, p.~852-861. ISSN~0006-3495. Available from: https://dx.doi.org/10.1016/j.bpj.2021.12.035.

Detailed Information on Publication Record