J 2022

Ancient Biosyntheses in an Oil Crop: Glucosinolate Profiles in Limnanthes alba and Its Relatives (Limnanthaceae, Brassicales)

AGERBIRK, Niels, David I. PATTISON, Terezie MALÍK MANDÁKOVÁ, Martin LYSÁK, Sabine MONTAUT et. al.

Basic information

Original name

Ancient Biosyntheses in an Oil Crop: Glucosinolate Profiles in Limnanthes alba and Its Relatives (Limnanthaceae, Brassicales)

Authors

AGERBIRK, Niels, David I. PATTISON, Terezie MALÍK MANDÁKOVÁ (203 Czech Republic, belonging to the institution), Martin LYSÁK (203 Czech Republic, guarantor, belonging to the institution), Sabine MONTAUT and Dan STAERK

Edition

Journal of Agricultural and Food Chemistry, American Chemical Society, 2022, 0021-8561

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10611 Plant sciences, botany

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 6.100

RIV identification code

RIV/00216224:14740/22:00125529

Organization unit

Central European Institute of Technology

DOI

UT WoS

000748132600001

Keywords in English

biosynthesis; evolution; authentic references; spiking; UHPLC; NMR; LC-QToF-MS; QToF MS/MS; mass spectrometry; fragmentation; Limnanthes; Floerkea; Limnanthaceae; Moringaceae; Brassicaceae; crucifers

Tags

Tags

International impact, Reviewed
Změněno: 19/12/2022 10:29, Mgr. Pavla Foltynová, Ph.D.

Abstract

V originále

The glucosinolate (GSL) profiles of four Limnanthaceae species, including the oil crop Limnanthes alba (meadowfoam), were investigated by an ultrahigh-performance liquid chromatography-quadrupole time-of -fight tandem mass spectrometry (UHPLC-QToF-MS/MS) analysis of desulfoGSLs after desulfation of native GSLs, supplemented by NMR of desulfated 2-hydroxy-2-methylpropylGSL and 3-methoxybenzylGSL. Leaves, roots, and seeds were investigated, providing an overview of biosynthetic capabilities in the genera Floerkea and Limnanthes. Methoxyl groups on benzylGSLs were in meta but not para positions; two 3,5-disubstituted benzylGSLs are tentatively proposed. 2-Hydroxy-2-methylpropylGSL was accompanied by an isomer that was not a previously reported GSL. The combined GSL profile of the family included GSLs derived from valine, leucine, isoleucine, phenylalanine, and tyrosine, and possibly methionine and tryptophan. Substituted indole GSLs and GSLs derived from chain-elongated amino acids or alanine were searched for but not detected. Hypothetic glycosides of GSLs were detected at low levels. Based on biochemical interpretation, we suggest biosynthetic schemes and gene families (CYP79C, GSOH) relevant for tailoring GSL profiles in Limnanthes crops.