Caspase-8 Deficient Osteoblastic Cells Display Alterations in Non-Apoptotic Pathways

@article{1841299,
   author = {Vesela, Barbora and Killinger, Michael and Říhová, Kamila and Beneš, Petr and Svandová, Eva and Kratochvílová, Adéla and Trčka, Filip and Kleparnik, Karel and Matalova, Eva},
   article_number = {March},
   doi = {http://dx.doi.org/10.3389/fcell.2022.794407},
   keywords = {osteogenesis; bone; CRISPR/cas9; apoptosis; autophagy; MC3T3-E1},
   language = {eng},
   issn = {2296-634X},
   journal = {Frontiers in Cell and Developmental Biology},
   title = {Caspase-8 Deficient Osteoblastic Cells Display Alterations in Non-Apoptotic Pathways},
   url = {https://www.frontiersin.org/articles/10.3389/fcell.2022.794407/full},
   volume = {10},
   year = {2022}
}

TY  - JOUR
ID  - 1841299
AU  - Vesela, Barbora - Killinger, Michael - Říhová, Kamila - Beneš, Petr - Svandová, Eva - Kratochvílová, Adéla - Trčka, Filip - Kleparnik, Karel - Matalova, Eva
PY  - 2022
TI  - Caspase-8 Deficient Osteoblastic Cells Display Alterations in Non-Apoptotic Pathways
JF  - Frontiers in Cell and Developmental Biology
VL  - 10
IS  - March
SP  - 1-11
EP  - 1-11
PB  - Frontiers Media SA
SN  - 2296634X
KW  - osteogenesis
KW  - bone
KW  - CRISPR/cas9
KW  - apoptosis
KW  - autophagy
KW  - MC3T3-E1
UR  - https://www.frontiersin.org/articles/10.3389/fcell.2022.794407/full
N2  - Caspase-8 is the key component of the receptor-mediated (extrinsic) apoptotic pathway. Immunological localization of active caspase-8 showed its presence in osteoblasts, including non-apoptotic ones. Further in vivo exploration of caspase-8 functions in the bone is hindered by the fact that the caspase-8 knock-out is lethal prenatally. Examinations were thus performed using individual cell populations in vitro. In this study, caspase-8 was eliminated by the CRISPR/cas9 technology in MC3T3-E1 cells, the most common in vitro model of osteoblastic populations. The aim of the work was to specify the consequences of caspase-8 deficiency on non-apoptotic pathways. The impact on the osteogenic gene expression of the osteoblastic cells along with alterations in proliferation, caspase cascades and rapamycin induced autophagy response were evaluated. Osteogenic differentiation of caspase-8 deficient cells was inhibited as these cells displayed a decreased level of mineralization and lower activity of alkaline phosphatase. Among affected osteogenic genes, based on the PCR Array, major changes were observed for Ctsk, as down-regulated, and Gdf10, as up-regulated. Other significantly down-regulated genes included those coding osteocalcin, bone morphogenetic proteins (-3, -4 and -7), collagens (-1a1, -14a1) or Phex. The formation of autophagosomes was not altered in rapamycin-treated caspase-8 deficient cells, but expression of some autophagy-related genes, including Tnfsf10, Cxcr4, Dapk1 and Igf1, was significantly downregulated. These data provide new insight into the effects of caspase-8 on non-apoptotic osteogenic pathways.
ER  - 

VESELA, Barbora, Michael KILLINGER, Kamila ŘÍHOVÁ, Petr BENEŠ, Eva SVANDOVÁ, Adéla KRATOCHVÍLOVÁ, Filip TRČKA, Karel KLEPARNIK and Eva MATALOVA. Caspase-8 Deficient Osteoblastic Cells Display Alterations in Non-Apoptotic Pathways. \textit{Frontiers in Cell and Developmental Biology}. Frontiers Media SA, 2022, vol.~10, March, p.~1-11. ISSN~2296-634X. Available from: https://dx.doi.org/10.3389/fcell.2022.794407.