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@article{1842605,
author = {Ranjan, N. and Pochopien, A.A. and Wu, C.C.C. and Beckert, B. and Blanchet, S. and Green, R. and Rodnina, M.V. and Wilson, D.N.},
article_location = {Hoboken (USA)},
article_number = {6},
doi = {http://dx.doi.org/10.15252/embj.2020106449},
keywords = {ABC ATPase; cryo‐ EM; eEF3; E‐ site tRNA; L1 stalk},
language = {eng},
issn = {0261-4189},
journal = {EMBO Journal},
title = {Yeast translation elongation factor eEF3 promotes late stages of tRNA translocation},
url = {https://www.embopress.org/doi/full/10.15252/embj.2020106449},
volume = {40},
year = {2021}
}
TY - JOUR
ID - 1842605
AU - Ranjan, N. - Pochopien, A.A. - Wu, C.C.C. - Beckert, B. - Blanchet, S. - Green, R. - Rodnina, M.V. - Wilson, D.N.
PY - 2021
TI - Yeast translation elongation factor eEF3 promotes late stages of tRNA translocation
JF - EMBO Journal
VL - 40
IS - 6
SP - 1-20
EP - 1-20
PB - WILEY-BLACKWELL
SN - 02614189
KW - ABC ATPase
KW - cryo‐ EM
KW - eEF3
KW - E‐ site tRNA
KW - L1 stalk
UR - https://www.embopress.org/doi/full/10.15252/embj.2020106449
N2 - In addition to the conserved translation elongation factors eEF1A and eEF2, fungi require a third essential elongation factor, eEF3. While eEF3 has been implicated in tRNA binding and release at the ribosomal A and E sites, its exact mechanism of action is unclear. Here, we show that eEF3 acts at the mRNA-tRNA translocation step by promoting the dissociation of the tRNA from the E site, but independent of aminoacyl-tRNA recruitment to the A site. Depletion of eEF3 in vivo leads to a general slowdown in translation elongation due to accumulation of ribosomes with an occupied A site. Cryo-EM analysis of native eEF3-ribosome complexes shows that eEF3 facilitates late steps of translocation by favoring non-rotated ribosomal states, as well as by opening the L1 stalk to release the E-site tRNA. Additionally, our analysis provides structural insights into novel translation elongation states, enabling presentation of a revised yeast translation elongation cycle.
ER -
RANJAN, N., A.A. POCHOPIEN, C.C.C. WU, B. BECKERT, S. BLANCHET, R. GREEN, M.V. RODNINA and D.N. WILSON. Yeast translation elongation factor eEF3 promotes late stages of tRNA translocation. \textit{EMBO Journal}. Hoboken (USA): WILEY-BLACKWELL, 2021, vol.~40, No~6, p.~1-20. ISSN~0261-4189. Available from: https://dx.doi.org/10.15252/embj.2020106449.
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