PECENKA, J., Z. BYTESNIKOVA, T. KISS, E. PENAZOVA, M. BARANEK, A. EICHMEIER, D. TEKIELSKA, L. RICHTERA, R. POKLUDA and V. ADAM. Silver nanoparticles eliminate Xanthomonas campestris pv. campestris in cabbage seeds more efficiently than hot water treatment. Materials Today Communications. Amsterdam: Elsevier Science, 2021, vol. 27, JUN, p. 102284-102291. ISSN 2352-4928. Available from: https://dx.doi.org/10.1016/j.mtcomm.2021.102284.
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Basic information
Original name Silver nanoparticles eliminate Xanthomonas campestris pv. campestris in cabbage seeds more efficiently than hot water treatment
Authors PECENKA, J., Z. BYTESNIKOVA, T. KISS, E. PENAZOVA, M. BARANEK, A. EICHMEIER, D. TEKIELSKA, L. RICHTERA, R. POKLUDA and V. ADAM.
Edition Materials Today Communications, Amsterdam, Elsevier Science, 2021, 2352-4928.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10611 Plant sciences, botany
Country of publisher Netherlands
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 3.662
RIV identification code RIV/00216224:14740/21:00124440
Organization unit Central European Institute of Technology
Doi http://dx.doi.org/10.1016/j.mtcomm.2021.102284
UT WoS 000683031900009
Keywords in English Plant protection; Nanomaterials; Pathogen; Antibiotics
Tags CF CRYO, ne MU, rivok
Tags International impact, Reviewed
Changed by Changed by: Mgr. Pavla Foltynová, Ph.D., učo 106624. Changed: 23/3/2022 12:06.
Abstract
Silver nanoparticles (AgNPs) and the standard hot water treatment (HWT) method were examined as measures against the bacterium Xanthomonas campestris pv. campestris (Xcc). Three sizes (similar to 2 nm, similar to 22 nm and similar to 29 nm) of AgNPs were synthesized and characterized using transmission electron microscopy and dynamic light scattering. To evaluate the direct effect of the synthesized AgNPs on Xcc, an in vitro colony counting assay was carried out. Cabbage seeds (cv.' Avak F1') were inoculated with an Xcc (strain 1279a) suspension and treated with hot water or a AgNP solution. The Xcc infestation in the young seedlings was quantified using real-time PCR and dilution plating assays. The results of the colony counting assay indicated that the antibacterial effect of the AgNPs depended on the size and concentration of the NPs. The seedling analysis showed that all three sizes of AgNPs suppressed Xcc infestation at concentrations of 6.25 mu g mL(-1) and higher. HWT also had an antibacterial effect; nevertheless, in contrast to AgNP treatment, HWT strongly decreased the germination rate of treated seeds.
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