Transcriptomic and Proteomic Analysis of Drought Stress
Response in Opium Poppy Plants during the First Week of
Germination

J 2021

Transcriptomic and Proteomic Analysis of Drought Stress Response in Opium Poppy Plants during the First Week of Germination

KUNDRATOVA, K., M. BARTAS, P. PECINKA, O. HEJNA, A. RYCHLA et. al.

Basic information

Original name

Transcriptomic and Proteomic Analysis of Drought Stress Response in Opium Poppy Plants during the First Week of Germination

Authors

KUNDRATOVA, K., M. BARTAS, P. PECINKA, O. HEJNA, A. RYCHLA, V. CURN and J. CERVEN

Edition

PLANTS-BASEL, BASEL, MDPI, 2021, 2223-7747

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10611 Plant sciences, botany

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.658

RIV identification code

RIV/00216224:14740/21:00124441

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.3390/plants10091878

UT WoS

000701583900001

Keywords in English

opium poppy; Papaver somniferum; drought response; transcriptomics; proteomics; dehydrins; gene expression; plant stress

Abstract

V originále

Water deficiency is one of the most significant abiotic stresses that negatively affects growth and reduces crop yields worldwide. Most research is focused on model plants and/or crops which are most agriculturally important. In this research, drought stress was applied to two drought stress contrasting varieties of Papaver somniferum (the opium poppy), a non-model plant species, during the first week of its germination, which differ in responses to drought stress. After sowing, the poppy seedlings were immediately subjected to drought stress for 7 days. We conducted a large-scale transcriptomic and proteomic analysis for drought stress response. At first, we found that the transcriptomic and proteomic profiles significantly differ. However, the most significant findings are the identification of key genes and proteins with significantly different expressions relating to drought stress, e.g., the heat-shock protein family, dehydration responsive element-binding transcription factors, ubiquitin E3 ligase, and others. In addition, metabolic pathway analysis showed that these genes and proteins were part of several biosynthetic pathways most significantly related to photosynthetic processes, and oxidative stress responses. A future study will focus on a detailed analysis of key genes and the development of selection markers for the determination of drought-resistant varieties and the breeding of new resistant lineages.

Links

90127, large research infrastructures

Name: CIISB II

Citovat

KUNDRATOVA, K., M. BARTAS, P. PECINKA, O. HEJNA, A. RYCHLA, V. CURN and J. CERVEN. Transcriptomic and Proteomic Analysis of Drought Stress Response in Opium Poppy Plants during the First Week of Germination. PLANTS-BASEL. BASEL: MDPI, 2021, vol. 10, No 9, p. 1878-1892. ISSN 2223-7747. Available from: https://dx.doi.org/10.3390/plants10091878.

@article{1842611,
   author = {Kundratova, K. and Bartas, M. and Pecinka, P. and Hejna, O. and Rychla, A. and Curn, V. and Cerven, J.},
   article_location = {BASEL},
   article_number = {9},
   doi = {http://dx.doi.org/10.3390/plants10091878},
   keywords = {opium poppy; Papaver somniferum; drought response; transcriptomics; proteomics; dehydrins; gene expression; plant stress},
   language = {eng},
   issn = {2223-7747},
   journal = {PLANTS-BASEL},
   title = {Transcriptomic and Proteomic Analysis of Drought Stress Response in Opium Poppy Plants during the First Week of Germination},
   url = {https://www.mdpi.com/2223-7747/10/9/1878},
   volume = {10},
   year = {2021}
}

TY  - JOUR
ID  - 1842611
AU  - Kundratova, K. - Bartas, M. - Pecinka, P. - Hejna, O. - Rychla, A. - Curn, V. - Cerven, J.
PY  - 2021
TI  - Transcriptomic and Proteomic Analysis of Drought Stress Response in Opium Poppy Plants during the First Week of Germination
JF  - PLANTS-BASEL
VL  - 10
IS  - 9
SP  - 1878
EP  - 1878
PB  - MDPI
SN  - 22237747
KW  - opium poppy
KW  - Papaver somniferum
KW  - drought response
KW  - transcriptomics
KW  - proteomics
KW  - dehydrins
KW  - gene expression
KW  - plant stress
UR  - https://www.mdpi.com/2223-7747/10/9/1878
N2  - Water deficiency is one of the most significant abiotic stresses that negatively affects growth and reduces crop yields worldwide. Most research is focused on model plants and/or crops which are most agriculturally important. In this research, drought stress was applied to two drought stress contrasting varieties of Papaver somniferum (the opium poppy), a non-model plant species, during the first week of its germination, which differ in responses to drought stress. After sowing, the poppy seedlings were immediately subjected to drought stress for 7 days. We conducted a large-scale transcriptomic and proteomic analysis for drought stress response. At first, we found that the transcriptomic and proteomic profiles significantly differ. However, the most significant findings are the identification of key genes and proteins with significantly different expressions relating to drought stress, e.g., the heat-shock protein family, dehydration responsive element-binding transcription factors, ubiquitin E3 ligase, and others. In addition, metabolic pathway analysis showed that these genes and proteins were part of several biosynthetic pathways most significantly related to photosynthetic processes, and oxidative stress responses. A future study will focus on a detailed analysis of key genes and the development of selection markers for the determination of drought-resistant varieties and the breeding of new resistant lineages.
ER  -

KUNDRATOVA, K., M. BARTAS, P. PECINKA, O. HEJNA, A. RYCHLA, V. CURN and J. CERVEN. Transcriptomic and Proteomic Analysis of Drought Stress Response in Opium Poppy Plants during the First Week of Germination. \textit{PLANTS-BASEL}. BASEL: MDPI, 2021, vol.~10, No~9, p.~1878-1892. ISSN~2223-7747. Available from: https://dx.doi.org/10.3390/plants10091878.

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