Lipid Polymorphism of the Subchloroplast-Granum and Stroma
Thylakoid Membrane-Particles. I. P-31-NMR Spectroscopy

J 2021

Lipid Polymorphism of the Subchloroplast-Granum and Stroma Thylakoid Membrane-Particles. I. P-31-NMR Spectroscopy

DLOUHY, O., U. JAVORNIK, O. ZSIROS, P. SKET, V. KARLICKY et. al.

Basic information

Original name

Lipid Polymorphism of the Subchloroplast-Granum and Stroma Thylakoid Membrane-Particles. I. P-31-NMR Spectroscopy

Authors

DLOUHY, O., U. JAVORNIK, O. ZSIROS, P. SKET, V. KARLICKY, V. SPUNDA, J. PLAVEC and G. GARAB

Edition

Blood Cells, Molecules and Diseases, Orlando, Florida, Academic Press, 2021, 1079-9796

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10601 Cell biology

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 2.372

RIV identification code

RIV/00216224:14740/21:00124445

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.3390/cells10092354

UT WoS

000699437700001

Keywords in English

P-31-NMR; bilayer membrane; DEM-dynamic exchange model; grana; H-II phase; isotropic phase; non-bilayer lipids; non-lamellar lipid phases; thylakoid membranes; structural flexibility

Abstract

V originále

Build-up of the energized state of thylakoid membranes and the synthesis of ATP are warranted by organizing their bulk lipids into a bilayer. However, the major lipid species of these membranes, monogalactosyldiacylglycerol, is a non-bilayer lipid. It has also been documented that fully functional thylakoid membranes, in addition to the bilayer, contain an inverted hexagonal (H-II) phase and two isotropic phases. To shed light on the origin of these non-lamellar phases, we performed P-31-NMR spectroscopy experiments on sub-chloroplast particles of spinach: stacked, granum and unstacked, stroma thylakoid membranes. These membranes exhibited similar lipid polymorphism as the whole thylakoids. Saturation transfer experiments, applying saturating pulses at characteristic frequencies at 5 degrees C, provided evidence for distinct lipid phases-with component spectra very similar to those derived from mathematical deconvolution of the P-31-NMR spectra. Wheat-germ lipase treatment of samples selectively eliminated the phases exhibiting sharp isotropic peaks, suggesting easier accessibility of these lipids compared to the bilayer and the H-II phases. Gradually increasing lipid exchanges were observed between the bilayer and the two isotropic phases upon gradually elevating the temperature from 5 to 35 degrees C, suggesting close connections between these lipid phases. Data concerning the identity and structural and functional roles of different lipid phases will be presented in the accompanying paper.

Links

90043, large research infrastructures

Name: CIISB

Citovat

DLOUHY, O., U. JAVORNIK, O. ZSIROS, P. SKET, V. KARLICKY, V. SPUNDA, J. PLAVEC and G. GARAB. Lipid Polymorphism of the Subchloroplast-Granum and Stroma Thylakoid Membrane-Particles. I. P-31-NMR Spectroscopy. Blood Cells, Molecules and Diseases. Orlando, Florida: Academic Press, 2021, vol. 10, No 9, p. 2354-2369. ISSN 1079-9796. Available from: https://dx.doi.org/10.3390/cells10092354.

@article{1842616,
   author = {Dlouhy, O. and Javornik, U. and Zsiros, O. and Sket, P. and Karlicky, V. and Spunda, V. and Plavec, J. and Garab, G.},
   article_location = {Orlando, Florida},
   article_number = {9},
   doi = {http://dx.doi.org/10.3390/cells10092354},
   keywords = {P-31-NMR; bilayer membrane; DEM-dynamic exchange model; grana; H-II phase; isotropic phase; non-bilayer lipids; non-lamellar lipid phases; thylakoid membranes; structural flexibility},
   language = {eng},
   issn = {1079-9796},
   journal = {Blood Cells, Molecules and Diseases},
   title = {Lipid Polymorphism of the Subchloroplast-Granum and Stroma Thylakoid Membrane-Particles. I. P-31-NMR Spectroscopy},
   url = {https://www.mdpi.com/2073-4409/10/9/2354},
   volume = {10},
   year = {2021}
}

TY  - JOUR
ID  - 1842616
AU  - Dlouhy, O. - Javornik, U. - Zsiros, O. - Sket, P. - Karlicky, V. - Spunda, V. - Plavec, J. - Garab, G.
PY  - 2021
TI  - Lipid Polymorphism of the Subchloroplast-Granum and Stroma Thylakoid Membrane-Particles. I. P-31-NMR Spectroscopy
JF  - Blood Cells, Molecules and Diseases
VL  - 10
IS  - 9
SP  - 2354
EP  - 2354
PB  - Academic Press
SN  - 10799796
KW  - P-31-NMR
KW  - bilayer membrane
KW  - DEM-dynamic exchange model
KW  - grana
KW  - H-II phase
KW  - isotropic phase
KW  - non-bilayer lipids
KW  - non-lamellar lipid phases
KW  - thylakoid membranes
KW  - structural flexibility
UR  - https://www.mdpi.com/2073-4409/10/9/2354
N2  - Build-up of the energized state of thylakoid membranes and the synthesis of ATP are warranted by organizing their bulk lipids into a bilayer. However, the major lipid species of these membranes, monogalactosyldiacylglycerol, is a non-bilayer lipid. It has also been documented that fully functional thylakoid membranes, in addition to the bilayer, contain an inverted hexagonal (H-II) phase and two isotropic phases. To shed light on the origin of these non-lamellar phases, we performed P-31-NMR spectroscopy experiments on sub-chloroplast particles of spinach: stacked, granum and unstacked, stroma thylakoid membranes. These membranes exhibited similar lipid polymorphism as the whole thylakoids. Saturation transfer experiments, applying saturating pulses at characteristic frequencies at 5 degrees C, provided evidence for distinct lipid phases-with component spectra very similar to those derived from mathematical deconvolution of the P-31-NMR spectra. Wheat-germ lipase treatment of samples selectively eliminated the phases exhibiting sharp isotropic peaks, suggesting easier accessibility of these lipids compared to the bilayer and the H-II phases. Gradually increasing lipid exchanges were observed between the bilayer and the two isotropic phases upon gradually elevating the temperature from 5 to 35 degrees C, suggesting close connections between these lipid phases. Data concerning the identity and structural and functional roles of different lipid phases will be presented in the accompanying paper.
ER  -

DLOUHY, O., U. JAVORNIK, O. ZSIROS, P. SKET, V. KARLICKY, V. SPUNDA, J. PLAVEC and G. GARAB. Lipid Polymorphism of the Subchloroplast-Granum and Stroma Thylakoid Membrane-Particles. I. P-31-NMR Spectroscopy. \textit{Blood Cells, Molecules and Diseases}. Orlando, Florida: Academic Press, 2021, vol.~10, No~9, p.~2354-2369. ISSN~1079-9796. Available from: https://dx.doi.org/10.3390/cells10092354.

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