Spawning Performance and Sex Steroid Levels in Female Pikeperch
Sander lucioperca Treated with Poly(lactic-co-glycolic acid)
Microparticles

J 2022

Spawning Performance and Sex Steroid Levels in Female Pikeperch Sander lucioperca Treated with Poly(lactic-co-glycolic acid) Microparticles

KNOWLES, J., Jakub VYSLOUŽIL, T. POLICAR, S. MILLA, Martina HOLICKÁ et. al.

Basic information

Original name

Spawning Performance and Sex Steroid Levels in Female Pikeperch Sander lucioperca Treated with Poly(lactic-co-glycolic acid) Microparticles

Authors

KNOWLES, J. (guarantor), Jakub VYSLOUŽIL (203 Czech Republic, belonging to the institution), T. POLICAR, S. MILLA, Martina HOLICKÁ (203 Czech Republic, belonging to the institution) and P. PODHOREC

Edition

ANIMALS, BASEL, MDPI, 2022, 2076-2615

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30104 Pharmacology and pharmacy

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.000

RIV identification code

RIV/00216224:14160/22:00119771

Organization unit

Faculty of Pharmacy

DOI

http://dx.doi.org/10.3390/ani12020208

UT WoS

000749805300001

Keywords in English

aquaculture; GnRHa; induced ovulation; reproductive dysfunction; sustained drug release

Abstract

V originále

Simple Summary Pikeperch Sander lucioperca is a promising candidate for intensive aquaculture. However, controlled reproduction has become the major bottleneck in pikeperch production. To improve and optimize its artificial reproduction, an effective method for hormone treatment is needed. The use of a poly(lactic-co-glycolic acid) microparticle-sustained-release system to administer gonadotropin-releasing hormone agonist to pikeperch resulted in acceptable reproductive output. Our results establish the potential of poly(lactic-co-glycolic acid) microparticle as a novel tool for hormone treatment in fish. Pikeperch Sander lucioperca is a piscivorous species considered a promising candidate for the diversification of intensive aquaculture. This study aimed to determine the effect of a sustained-release delivery system incorporating mammalian gonadotropin-releasing hormone agonist (mGnRHa) into poly(lactic-co-glycolic acid) (PLGA) microparticles on the sex steroid levels and aspects of artificial reproduction of pikeperch. Fish were divided into four groups and injected with 20 mu g mGnRHa/kg, 5-day release microparticles encapsulated with 5 mu g GnRHa/kg BW (PLGA 5), 20 mu g GnRHa/kg (PLGA 20), or 1 mL/kg 0.9% NaCl (control). Cumulative percentage ovulation was 100% in the PLGA 5 group, significantly higher than in other tested groups. No differences among groups were observed in latency or fecundity. The level of 11-ketotestosterone (11-KT) peaked at 40 h post-injection, and was sustained during ovulation, in all treated groups. The 17 beta-estradiol (E2) concentration increased in the mGnRHa-only group immediately after hormone injection, while both PLGA groups showed a reduction in E2 after injection, continuing to decrease until ovulation. A low dose of mGnRHa in PLGA microparticles significantly improves induction of ovulation and results in acceptable reproductive performance, which may positively affect pikeperch production under controlled conditions.

Links

QK1810221, research and development project

Name: Využití mikročástic jako nosičů hormonálně aktivních látek v řízené reprodukci ryb.

Investor: Ministry of Agriculture of the CR

Citovat

KNOWLES, J., Jakub VYSLOUŽIL, T. POLICAR, S. MILLA, Martina HOLICKÁ and P. PODHOREC. Spawning Performance and Sex Steroid Levels in Female Pikeperch Sander lucioperca Treated with Poly(lactic-co-glycolic acid) Microparticles. ANIMALS. BASEL: MDPI, 2022, vol. 12, No 2, p. 1-11. ISSN 2076-2615. Available from: https://dx.doi.org/10.3390/ani12020208.

@article{1842639,
   author = {Knowles, J. and Vysloužil, Jakub and Policar, T. and Milla, S. and Holická, Martina and Podhorec, P.},
   article_location = {BASEL},
   article_number = {2},
   doi = {http://dx.doi.org/10.3390/ani12020208},
   keywords = {aquaculture; GnRHa; induced ovulation; reproductive dysfunction; sustained drug release},
   language = {eng},
   issn = {2076-2615},
   journal = {ANIMALS},
   title = {Spawning Performance and Sex Steroid Levels in Female Pikeperch Sander lucioperca Treated with Poly(lactic-co-glycolic acid) Microparticles},
   url = {https://www.mdpi.com/2076-2615/12/2/208},
   volume = {12},
   year = {2022}
}

TY  - JOUR
ID  - 1842639
AU  - Knowles, J. - Vysloužil, Jakub - Policar, T. - Milla, S. - Holická, Martina - Podhorec, P.
PY  - 2022
TI  - Spawning Performance and Sex Steroid Levels in Female Pikeperch Sander lucioperca Treated with Poly(lactic-co-glycolic acid) Microparticles
JF  - ANIMALS
VL  - 12
IS  - 2
SP  - 1-11
EP  - 1-11
PB  - MDPI
SN  - 20762615
KW  - aquaculture
KW  - GnRHa
KW  - induced ovulation
KW  - reproductive dysfunction
KW  - sustained drug release
UR  - https://www.mdpi.com/2076-2615/12/2/208
N2  - Simple Summary Pikeperch Sander lucioperca is a promising candidate for intensive aquaculture. However, controlled reproduction has become the major bottleneck in pikeperch production. To improve and optimize its artificial reproduction, an effective method for hormone treatment is needed. The use of a poly(lactic-co-glycolic acid) microparticle-sustained-release system to administer gonadotropin-releasing hormone agonist to pikeperch resulted in acceptable reproductive output. Our results establish the potential of poly(lactic-co-glycolic acid) microparticle as a novel tool for hormone treatment in fish. Pikeperch Sander lucioperca is a piscivorous species considered a promising candidate for the diversification of intensive aquaculture. This study aimed to determine the effect of a sustained-release delivery system incorporating mammalian gonadotropin-releasing hormone agonist (mGnRHa) into poly(lactic-co-glycolic acid) (PLGA) microparticles on the sex steroid levels and aspects of artificial reproduction of pikeperch. Fish were divided into four groups and injected with 20 mu g mGnRHa/kg, 5-day release microparticles encapsulated with 5 mu g GnRHa/kg BW (PLGA 5), 20 mu g GnRHa/kg (PLGA 20), or 1 mL/kg 0.9% NaCl (control). Cumulative percentage ovulation was 100% in the PLGA 5 group, significantly higher than in other tested groups. No differences among groups were observed in latency or fecundity. The level of 11-ketotestosterone (11-KT) peaked at 40 h post-injection, and was sustained during ovulation, in all treated groups. The 17 beta-estradiol (E2) concentration increased in the mGnRHa-only group immediately after hormone injection, while both PLGA groups showed a reduction in E2 after injection, continuing to decrease until ovulation. A low dose of mGnRHa in PLGA microparticles significantly improves induction of ovulation and results in acceptable reproductive performance, which may positively affect pikeperch production under controlled conditions.
ER  -

KNOWLES, J., Jakub VYSLOUŽIL, T. POLICAR, S. MILLA, Martina HOLICKÁ and P. PODHOREC. Spawning Performance and Sex Steroid Levels in Female Pikeperch Sander lucioperca Treated with Poly(lactic-co-glycolic acid) Microparticles. \textit{ANIMALS}. BASEL: MDPI, 2022, vol.~12, No~2, p.~1-11. ISSN~2076-2615. Available from: https://dx.doi.org/10.3390/ani12020208.

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