TIME-LAPSE MONITORING OF CELL MECHANICAL PROPERTIES

D 2021

TIME-LAPSE MONITORING OF CELL MECHANICAL PROPERTIES

PŘIBYL, Jan, Vladimír ROTREKL, Martin PEŠL, Šárka JELÍNKOVÁ, I. KRATOCHVILOVA et. al.

Basic information

Original name

TIME-LAPSE MONITORING OF CELL MECHANICAL PROPERTIES

Authors

PŘIBYL, Jan (203 Czech Republic, belonging to the institution), Vladimír ROTREKL (203 Czech Republic, belonging to the institution), Martin PEŠL (203 Czech Republic, belonging to the institution), Šárka JELÍNKOVÁ (203 Czech Republic, belonging to the institution) and I. KRATOCHVILOVA

Edition

SLEZSKA, CONFERENCE PROCEEDINGS - NANOCON 2020, p. 416-421, 6 pp. 2021

Publisher

TANGER LTD

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

10601 Cell biology

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

Publication form

electronic version available online

References:

URL

RIV identification code

RIV/00216224:14740/21:00120237

Organization unit

Central European Institute of Technology

ISBN

978-80-87294-98-7

ISSN

DOI

http://dx.doi.org/10.37904/nanocon.2020.3740

UT WoS

000664505500071

Keywords in English

Atomic Force Microscopy; Mechanical Mapping; Cell stiffness; Cryopreservation

Abstract

V originále

Atomic force microscopy (AFM) is a highly sensitive non-invasive surface method able to provide insight into cells' mechanical parameters. Membrane and sub-membrane development, as well as internal cellular properties, can be monitored. The stiffness of cells is a fundamental phenomenon that reflects changes in cell physiology. More importantly, changes in cell mechanical properties are also often found to be closely associated with various disease conditions. Cell mechanics are mainly dependent on cytoskeletal architecture. The development of cryopreserved cells' mechanical properties (stiffness) after thawing was studied using AFM. Cell stiffness was mapped and thus monitored in time and space under nearly physiological conditions (i.e., in culture medium and at elevated temperature). In AFM force spectroscopy mode, cells are indented at many sites, and their complete elastic responses are recorded, enabling them to reconstruct a stiffness map. We measured the frozen cell surface stiffness immediately after thawing; they, when the dynamics of development of the cell stiffness were monitored in time up to 24 hours. Moreover, the AFM spectroscopy was combined with fluorescence-based staining of the cytoskeleton, thus enabling to directly correlate cytoskeleton development with stiffness mapping.

Links

LM2018127, research and development project

Name: Česká infrastruktura pro integrativní strukturní biologii (Acronym: CIISB)

Investor: Ministry of Education, Youth and Sports of the CR

NU20-06-00156, research and development project

Name: Vliv pneumologické medikace na funkce lidských kardiomyocytů.

Investor: Ministry of Health of the CR, Subprogram 1 - standard

Citovat

PŘIBYL, Jan, Vladimír ROTREKL, Martin PEŠL, Šárka JELÍNKOVÁ and I. KRATOCHVILOVA. TIME-LAPSE MONITORING OF CELL MECHANICAL PROPERTIES. Online. In CONFERENCE PROCEEDINGS - NANOCON 2020. SLEZSKA: TANGER LTD, 2021, p. 416-421. ISBN 978-80-87294-98-7. Available from: https://dx.doi.org/10.37904/nanocon.2020.3740.

@inproceedings{1842662,
   author = {Přibyl, Jan and Rotrekl, Vladimír and Pešl, Martin and Jelínková, Šárka and Kratochvilova, I.},
   address = {SLEZSKA},
   booktitle = {CONFERENCE PROCEEDINGS - NANOCON 2020},
   doi = {http://dx.doi.org/10.37904/nanocon.2020.3740},
   keywords = {Atomic Force Microscopy; Mechanical Mapping; Cell stiffness; Cryopreservation},
   howpublished = {elektronická verze "online"},
   language = {eng},
   location = {SLEZSKA},
   isbn = {978-80-87294-98-7},
   pages = {416-421},
   publisher = {TANGER LTD},
   title = {TIME-LAPSE MONITORING OF CELL MECHANICAL PROPERTIES},
   url = {https://www.confer.cz/nanocon/2020},
   year = {2021}
}

TY  - JOUR
ID  - 1842662
AU  - Přibyl, Jan - Rotrekl, Vladimír - Pešl, Martin - Jelínková, Šárka - Kratochvilova, I.
PY  - 2021
TI  - TIME-LAPSE MONITORING OF CELL MECHANICAL PROPERTIES
PB  - TANGER LTD
CY  - SLEZSKA
SN  - 9788087294987
KW  - Atomic Force Microscopy
KW  - Mechanical Mapping
KW  - Cell stiffness
KW  - Cryopreservation
UR  - https://www.confer.cz/nanocon/2020
N2  - Atomic force microscopy (AFM) is a highly sensitive non-invasive surface method able to provide insight into cells' mechanical parameters. Membrane and sub-membrane development, as well as internal cellular properties, can be monitored. The stiffness of cells is a fundamental phenomenon that reflects changes in cell physiology. More importantly, changes in cell mechanical properties are also often found to be closely associated with various disease conditions. Cell mechanics are mainly dependent on cytoskeletal architecture. The development of cryopreserved cells' mechanical properties (stiffness) after thawing was studied using AFM. Cell stiffness was mapped and thus monitored in time and space under nearly physiological conditions (i.e., in culture medium and at elevated temperature). In AFM force spectroscopy mode, cells are indented at many sites, and their complete elastic responses are recorded, enabling them to reconstruct a stiffness map. We measured the frozen cell surface stiffness immediately after thawing; they, when the dynamics of development of the cell stiffness were monitored in time up to 24 hours. Moreover, the AFM spectroscopy was combined with fluorescence-based staining of the cytoskeleton, thus enabling to directly correlate cytoskeleton development with stiffness mapping.
ER  -

PŘIBYL, Jan, Vladimír ROTREKL, Martin PEŠL, Šárka JELÍNKOVÁ and I. KRATOCHVILOVA. TIME-LAPSE MONITORING OF CELL MECHANICAL PROPERTIES. Online. In \textit{CONFERENCE PROCEEDINGS - NANOCON 2020}. SLEZSKA: TANGER LTD, 2021, p.~416-421. ISBN~978-80-87294-98-7. Available from: https://dx.doi.org/10.37904/nanocon.2020.3740.

Detailed Information on Publication Record